FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-164-6-7-4-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-164-6-7-4-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45727
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA2186847.822949242242004No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA34247.487917422966738No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA22494.918319592363374No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA15873.470597240142585No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA15833.4618496730596804No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA11712.5608502635204586No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGA8881.941959892404925No Hit
TCGGTCAAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC7281.5920572090887222No Hit
TCGGTCAAACTGGGATTAGATACCCCAGTAGTCTACGCCCTAAACGATGA7081.548319373674197No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA6811.4892732958645878No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA6631.4499092439915149No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGG6541.4302272180549784No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA4420.9666061626610098No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA3740.8178975222516238No Hit
TCGGTCAAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA3550.7763465786078247No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA2950.6451330723642487No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA2810.614516587574081No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA2050.4483128129988847No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCATAAACTTTGA1850.4045749775843594No Hit
TCGGTCAAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA1810.39582741050145426No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGAGTTACACTAACAAAACCA1700.37177160102346535No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGTATGGTCGTTAACATTGA1610.35208957508692895No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA1580.34552889977475015No Hit
TCGGTCAAACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAA1440.31491241498458244No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAA1430.31272552321385616No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGATTAACCCTAAACTCGAA1370.2996041725894985No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAG1320.28866971373586725No Hit
TCGGTCAAACTGGGATTAGATACCCCTGTAGTCCACGCCCTAAACGATGG1310.2864828219651409No Hit
TCGGTCAAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1220.26680079602860457No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTAA1180.2580532289456995No Hit
TCGGTCAAACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACTATAA1180.2580532289456995No Hit
TCGGTCAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1130.24711877009206815No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA1000.2186891770726267No Hit
TCGGTCAAACTGGGATTAGATACCCCGGTAGTCCTGGCCCTAAACGCTGC950.20775471821899535No Hit
TCGGTCAAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAACTT930.20338093467754279No Hit
TCGGTCAAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAG900.19682025936536401No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCGTAAACTTAGA880.19244647582391147No Hit
TCGGTCAAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCT860.18807269228245893No Hit
TCGGTCAAACTGGGATTAGATACCCCGGTAGTCCACGCCCTAAACGCTGT670.14652174863865988No Hit
TCGGTCAAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC660.14433485686793363No Hit
TCGGTCAAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTT640.13996107332648108No Hit
TCGGTCAAACTGGGATTAGATACCCCTGTAGTCCACGCCCTAAACAATGA640.13996107332648108No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCCAAA630.1377741815557548No Hit
TCGGTCAAACTGGGATTAGATACCCCCCCTGGCCCTGATTGGTGTATCTG620.13558728978502854No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA600.131213506243576No Hit
TCGGTCAAACTGGGATTAGATACCCCAGTAGTCCACGCCCTAAACGATCT600.131213506243576No Hit
TCGGTAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC590.12902661447284974No Hit
TCGGTCAAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTT570.1246528309313972No Hit
TCGGTCAAACTGGGATTAGATACCCCGTTTACCTATAAGAGAATGCTGAT550.12027904738994466No Hit
TCGGTCAAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGC540.1180921556192184No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA510.1115314803070396No Hit
TCGGTCAAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT490.10715769676558706No Hit
TCGGTCAAACTGGGATTAGATACCCCCTCTCTTGGAGAGTTTGCTTCGTA460.10059702145340826No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAGA108.438499E-4295.09
AACTGAG157.195049E-6295.08
GTCAACT303.6379788E-12295.04
GTCAAAT108.438499E-4295.04
GATGTTG157.195049E-6295.0295
TCGGTCA45600.0295.01
GGTCAAA45250.0295.03
CGGTCAA45600.0295.02
CGGTAAA108.438499E-4295.02
GTCAAAC45250.0294.348084
CAAACTG45100.0294.34596
AAACTGG45000.0294.344457
TCAAACT45350.0293.373785
AACTGGG45000.0293.033338
ACTGGGA45000.0293.033339
GGTCAAC400.0258.1253
CAACTGG351.2732926E-11252.857136
AACTGGA251.8626088E-7236.08
ACTGGAT251.8626088E-7236.09
TCAACTG403.274181E-11221.255
Produced by FastQC (version 0.11.5)