FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-163-6-1-1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-163-6-1-1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43715
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2001245.77833695527851No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG22675.185862976095161No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCTGT11352.596362804529338No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG11302.5849250829234816No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT11172.555187006748256No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC10332.363033283769873No Hit
GGTACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT10222.3378702962369897No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG9502.1731671051126615No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG8952.047352167448244No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG7791.7819970261923823No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG7461.706508063593732No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG6061.386251858629761No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT5661.2947500857829122No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG5431.242136566395974No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG4481.0248198558847077No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG3330.7617522589500172No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACC3240.7411643600594762No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG3110.7114262838842503No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATGC2950.6748255747455107No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG2030.46437149719775817No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACCA1920.43920850966487474No Hit
GGTACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTACCACT1820.4163330664531626No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA1740.3980327118837928No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATAC1730.39574516756262157No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG1690.38659499027793665No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1600.3660070913873956No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACCACTCCT1360.3111060276792863No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCCTG1310.29966830607343015No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT1260.2882305844675741No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG1220.2790804071828892No Hit
GGTACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATAG1090.24934233100766326No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTAG1060.24247969804414962No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG1010.23104197643829347No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTATAATAG990.22646688779595103No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCACTC980.22417934347477983No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG930.2127416218689237No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTAA910.2081665332265813No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACCACTCC900.20587898890541004No Hit
GGTACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATAA870.1990163559418964No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT830.18986617865721148No Hit
GGTACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTACCA690.15784055816081438No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG680.15555301383964315No Hit
GGTACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTACCAC670.15326546951847192No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCCAAACCTCAACAG670.15326546951847192No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTACC660.15097792519730072No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG640.14640283655495825No Hit
GGTACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATTA640.14640283655495825No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG620.14182774791261582No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACTCC620.14182774791261582No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATGA620.14182774791261582No Hit
GGTACACTGGGATTAGATACCCCAATACGCTTAGCCCTAAACTCCAATAG620.14182774791261582No Hit
GGTACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTACCACTCC610.1395402035914446No Hit
GGTACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT600.13725265927027336No Hit
GGTACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTACCACTCC590.13496511494910216No Hit
GGTACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTACC580.13267757062793092No Hit
GGTACACTGGGATTAGATACCCCCACCAAGACCGGGTAAACTTAACATTC580.13267757062793092No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATAG570.1303900263067597No Hit
GGTACACTGGGATTAGATACCCCAAGAGTGCTGGCGGAATTCTTCTAAGT540.12352739334324603No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACAG530.12123984902207481No Hit
GGTACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTACCACTC500.11437721605856113No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG490.11208967173738991No Hit
GGTACATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT480.10980212741621868No Hit
GGTACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATAGA460.10522703877387625No Hit
GGTACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTACCACTCCT450.10293949445270502No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCGATAG450.10293949445270502No Hit
GGTACACTGGGATTAGATACCCCGTCTCGTCGTCGTCGTCGTCTAGAGGA440.1006519501315338No Hit
GGTACACTGGGATTAGATACCCCACCGACCAAAAAAGGTTTCCAATTACC440.1006519501315338No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT108.437236E-4295.06
GGGTTAG206.117443E-8295.09
ACATGGG108.437236E-4295.04
CTGGGTT206.117443E-8295.07
CTGGATT255.2204996E-10295.07
TGGATTA255.2204996E-10295.08
TGGGTTA206.117443E-8295.08
CATGGGA108.437236E-4295.05
ACTGGGA42950.0295.06
GGTACAC43550.0295.01
GGGATTG157.1928953E-6294.999979
TGGGATA303.6379788E-12294.999978
TACACTG43500.0294.66093
ACACTGG43450.0294.660524
CACTGGG43200.0294.658545
CTGGGAT43000.0294.656987
GTACACT43600.0294.32342
TGGGATT42800.0293.966138
GGGATTA42700.0293.272869
GGGATAG351.2732926E-11252.857139
Produced by FastQC (version 0.11.5)