FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-162-5-1-3-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-162-5-1-3-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43970
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC983422.365249033431887No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA458810.434387082101432No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAA35668.110075051171254No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT23395.319536047304981No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTGA19564.4484876051853535No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT9912.2538094155105752No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA9332.1219012963384123No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT8601.955879008414828No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGA8511.9354105071639753No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTGA7681.746645440072777No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA7411.6852399363202184No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTG7281.65567432340232No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT6441.46463497839436No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCTG6301.4327950875596998No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT5751.3077098021378213No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGAG5621.2781441892199228No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT4300.977939504207414No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC4200.9551967250397999No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT4190.9529224471230384No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT3000.6822833750284285No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGC2630.5981350921082557No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT2520.5731180350238799No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT2400.5458267000227428No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC2290.5208096429383671No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT2100.47759836251989995No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT1780.40482146918353423No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGCTGA1630.3707073004321128No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT1630.3707073004321128No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAGC1620.36843302251535137No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCTG1600.3638844666818285No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1530.34796452126449856No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAG1510.34341596543097563No Hit
ATCGGTTACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTA1500.34114168751421425No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT1480.3365931316806914No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT1460.3320445758471685No Hit
ATCGGTTACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAGC1410.3206731862633614No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC1350.30702751876279283No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGCT1300.29565612917898565No Hit
ATCGGTTACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT1150.2615419604275642No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAG1130.2569934045940414No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT1090.2478962929269957No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAG1060.24107345917671139No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGCTG1050.23879918125994998No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1040.23652490334318854No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT1000.22742779167614283No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG950.2160564020923357No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAG950.2160564020923357No Hit
ATCGGTTACTGGGATTAGATACCCCACGAACTAGAGGAGCCTGTTCTAGC950.2160564020923357No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCTAGAGGAGCCTGTTCTAGC910.20695929042529No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT900.20468501250852852No Hit
ATCGGTTACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGCTGA890.20241073459176712No Hit
ATCGGTTACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGCT870.19786217875824427No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGC840.19103934500795997No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT780.1773936775073914No Hit
ATCGGTTACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA780.1773936775073914No Hit
ATCGGTTACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGCTG760.17284512167386853No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA720.16374801000682285No Hit
ATCGGTTACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTA690.15692517625653854No Hit
ATCGGTTACTGGGATTAGATACCCCTGTGCTAGAGGAGCCTGTTCTAGCT680.15465089833977713No Hit
ATCGGTTACTGGGATTAGATACCCCCCGAGACCTAGAGGAGCCTGTTCTA650.14782806458949282No Hit
ATCGGTTACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGCT650.14782806458949282No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA650.14782806458949282No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG640.14555378667273142No Hit
ATCGGTTACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAG630.14327950875596998No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT630.14327950875596998No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGCT620.14100523083920855No Hit
ATCGGTTACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC600.1364566750056857No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTG580.13190811917216283No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC570.12963384125540142No Hit
ATCGGTTACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTAG550.12508528542187855No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGAACTAGAGGAGCCTGTTCTA550.12508528542187855No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGCT540.12281100750511711No Hit
ATCGGTTACTGGGATTAGATACCCCGACGAACCTAGAGGAGCCTGTTCTA510.11598817375483284No Hit
ATCGGTTACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGCTGA510.11598817375483284No Hit
ATCGGTTACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA510.11598817375483284No Hit
ATCGGTTACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGCT500.11371389583807141No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTAGC490.11143961792130998No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGCTAGAGGAGCCTGTTCTAGCT490.11143961792130998No Hit
ATCGGTTACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGCTGA480.10916534000454856No Hit
ATCGGTTACTGGGATTAGATACCCCTACCCTAGAGGAGCCTGTTCTAGCT470.10689106208778712No Hit
ATCGGTTACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTC460.1046167841710257No Hit
ATCGGTTACTGGGATTAGATACCCCCACGGACCTAGAGGAGCCTGTTCTA450.10234250625426426No Hit
ATCGGTTACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGC440.10006822833750284No Hit
ATCGGTTACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGCTGAG440.10006822833750284No Hit
ATCGGTTACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGCT440.10006822833750284No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC43550.0295.03
TTACGGG157.1931754E-6295.06
ATCGGTT43700.0295.01
TCGGTTA43650.0295.02
TACTGGA206.117807E-8294.999977
GTACTGG206.117807E-8294.999975
ATCGGTA206.117807E-8294.999971
GGTTATG108.4374007E-4294.999974
GTTATGG108.4374007E-4294.999975
CTGGATT206.117807E-8294.999979
TTACTGG43300.0294.659366
GTTACTG43300.0294.659365
GGTTACT43500.0293.982734
CTGGGAT43200.0293.634289
ACTGGGA43350.0292.958478
TACTGGG43300.0292.956127
GGTACTG251.8617902E-7236.04
ACTGGAT251.8617902E-7236.08
TACGGGA202.2672726E-5221.249987
ACGGGAT202.2672726E-5221.249988
Produced by FastQC (version 0.11.5)