FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-161-6-7-4-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-161-6-7-4-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42152
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA2200852.211045739229455No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA19444.611880812298349No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA17554.163503511102676No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA16453.9025431770734484No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA15773.741222243309926No Hit
TCGGTCAAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA13413.1813437084835834No Hit
TCGGTCAAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA6521.5467830707914216No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA6501.5420383374454356No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGG5741.3617384702979694No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA4651.1031505029417348No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA4641.1007781362687419No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGA4321.0248624027329665No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA3360.7971152021256405No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGA3150.747295501992788No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAG2850.6761245018029987No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATA2670.633421901689125No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGA2580.6120706016321882No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGA2480.5883469349022585No Hit
TCGGTCAAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACAGA2020.4792180679445815No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA2010.4768457012715885No Hit
TCGGTCAAACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAAT1970.4673562345796166No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGG1780.4222812677927501No Hit
TCGGTCAAACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAA1700.4033023344088062No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA1580.37483393433289053No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGTATGGTCGTTAACATTGA1410.33450370089200987No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAA1230.29180110077813626No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA1180.2799392674131714No Hit
TCGGTCAAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1170.2775669007401784No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCTCTAAACCTCAA1090.25858796735623457No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCTAA1080.2562156006832416No Hit
TCGGTCAAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC990.23486430062630478No Hit
TCGGTCAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC990.23486430062630478No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA990.23486430062630478No Hit
TCGGTCAAACTGGGATTAGATACCCCCGCCCCCATCAGGAAAATGACGGG980.2324919339533118No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAA960.22774720060732587No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA920.21825773391535397No Hit
TCGGTCAAACTGGGATTAGATACCCCAGTAGTCCACGCCCTAAACGTTCC910.215885367242361No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAA820.19453406718542418No Hit
TCGGTCAAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC760.18029986714746632No Hit
TCGGTCAAACTGGGATTAGATACCCCAGTAGTCTACGCCCTAAACGATGA690.16369330043651548No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGACCTAAACCTCAA670.1589485670905295No Hit
TCGGTCAAACTGGGATTAGATACCCCGGTAGTCCACGCCCTAAACGCTGT660.15657620041753653No Hit
TCGGTAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC610.14471436705257165No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA590.1399696337065857No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA550.1304801670146138No Hit
TCGGTCAAACTGGGATTAGATACCCCAGAGATGATGGCGTCGAACTCCTT540.1281078003416208No Hit
TCGGTCAAACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAAC520.12336306699563485No Hit
TCGGTCAAACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC510.12099070032264188No Hit
TCGGTCAAACTGGGATTAGATACCCCTGTAGTCCACGCCCTAAACGATGA490.1162459669766559No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAA470.11150123363066995No Hit
TCGGTCAAACTGGGATTAGATACCCCGAGAGTTTTCGGAAGATCGTGGCC430.10201176693869804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGA206.115806E-8294.99178
CAACTGG400.0294.99176
ACTGGGT157.191893E-6294.99179
ACTGGAT108.436885E-4294.99179
ACTGGAA108.436885E-4294.99179
TCGGTCA42050.0294.991671
CGGTCAA42100.0294.641362
GGTCAAA41700.0294.637973
AAACTGG41400.0294.63547
GTCAAAC41700.0294.284274
TCAAACT41700.0293.930575
CAAACTG41650.0293.221046
AACTGGG41550.0291.796848
ACTGGGA41350.0291.78149
GTCAACT450.0262.214844
GGTCAAC500.0235.993333
AACTAGG202.2668688E-5221.243778
TCAACTG550.0214.539435
ACTGATT150.002840247196.661139
GCTAACA200.0067154127147.49585295
Produced by FastQC (version 0.11.5)