FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-160-6-7-4-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-160-6-7-4-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42025
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA2396457.023200475907196No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA18384.373587150505651No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA17624.192742415229031No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA14283.39797739440809No Hit
TCGGTCAAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC10522.5032718619869128No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA10282.446162998215348No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA7691.8298631766805473No Hit
TCGGTCAAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA6851.6299821534800714No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA5911.4063057703747768No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA5571.325401546698394No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA4871.158834027364664No Hit
TCGGTCAAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA3080.7328970850684117No Hit
TCGGTCAAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAA2880.6853063652587745No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA2800.6662700773349197No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA2340.5568114217727543No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA2290.544913741820345No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA1830.43545508625817964No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAA1730.4116597263533611No Hit
TCGGTCAAACTGGGATTAGATACCCCGAGAGTTTTCGGAAGATCGTGGCC1700.4045211183819155No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAA1640.3902439024390244No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA1510.3593099345627603No Hit
TCGGTCAAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAG1480.3521713265913147No Hit
TCGGTCAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1460.34741225461035097No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAG1210.2879238548483046No Hit
TCGGTCAAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1160.2760261748958953No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCATAAACTTAAA1130.26888756692444976No Hit
TCGGTCAAACTGGGATTAGATACCCCACTACGCTTAGCCCTAAAGTCGAA1120.26650803093396785No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA1110.26412849494348606No Hit
TCGGTCAAACTGGGATTAGATACCCCTGTAGTCCACGCCCTAAACGATGA1070.2546103509815586No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCCAGCCGTAAACCTTGA910.2165377751338489No Hit
TCGGTCAAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC900.21415823914336707No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCTCTAAACTCCAA880.2093991671624033No Hit
TCGGTCAAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT800.19036287923854847No Hit
TCGGTCAAACTGGGATTAGATACCCCAGTGGTGGTGGCTGTTGCTATCTG740.17608566329565736No Hit
TCGGTCAAACTGGGATTAGATACCCCGGTAGTCCTGGCCCTAAACGATGA720.17132659131469363No Hit
TCGGTCAAACTGGGATTAGATACCCCAGGCCGCTGCATTTCTGGCAGGCG720.17132659131469363No Hit
TCGGTCAAACTGGGATTAGATACCCCAGTAGTCTACGCCCTAAACGATGA630.14991076740035691No Hit
TCGGTCAAACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAAT620.1475312314098751No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA620.1475312314098751No Hit
TCGGTAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC590.1403926234384295No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGCAAACTTTGA540.12849494348602022No Hit
TCGGTCAAACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAAC480.11421772754312909No Hit
TCGGTCAAACTGGGATTAGATACCCCTGTAGTCCACGCCCTAAACAATGA480.11421772754312909No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA470.11183819155264722No Hit
TCGGTCAAACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC440.10469958358120166No Hit
TCGGTCAAACTGGGATTAGATACCCCTCAATACCATTTCCACGGAGAAAG430.10232004759071982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTCA41900.0294.960721
CGGTCAA41850.0294.960722
GGTCAAC400.0294.96073
GATGAAA108.439433E-4294.9607295
ACGGGAT108.439433E-4294.96079
ACTGGGT157.194727E-6294.96079
AACGGGA108.439433E-4294.96078
AAACGGG108.439433E-4294.96077
GGTCAAA41400.0294.60453
AAACTGG41150.0294.602337
GTCAAAC41450.0293.89334
TCAAACT41400.0293.179575
CAAACTG41350.0293.17746
AACTGGG41100.0292.448888
ACTGGGA40950.0292.439679
AACTGGA351.2732926E-11252.823468
CAACTGG351.2732926E-11252.823466
GTCAACT351.2732926E-11252.823464
ACTGGAT351.2732926E-11252.823469
TCAACTG403.274181E-11221.220525
Produced by FastQC (version 0.11.5)