FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-16-4-2-6-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-16-4-2-6-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12005
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA232419.35860058309038No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAT149012.41149521032903No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACTG9748.113286130778842No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGCA5934.939608496459808No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA4954.123281965847563No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA2762.299042065805914No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTATA2532.1074552269887548No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCACT2381.9825072886297375No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA2161.7992503123698458No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAGC2041.699291961682632No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT1761.4660558100791339No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATAAGC1651.3744273219491878No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1301.0828821324448146No Hit
GCCACAACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTATAAG1261.0495626822157436No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAT1251.0412328196584755No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAT1180.982923781757601No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1130.9412744689712619No Hit
GCCACAACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTATAAG1110.9246147438567264No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATAAGCAC1080.8996251561849229No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAGCA1070.8912952936276551No Hit
GCCACAACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTATA1040.8663057059558518No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1020.849645980841316No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATAAGC1000.8329862557267805No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA940.7830070803831737No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA940.7830070803831737No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA920.7663473552686381No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA730.6080799666805498No Hit
GCCACAACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTATA660.5497709287796751No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATAA600.49979175343606835No Hit
GCCACAACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTATAA520.4331528529779259No Hit
GCCACAACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTAT520.4331528529779259No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT500.41649312786339027No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA490.40816326530612246No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA490.40816326530612246No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATAAGCA480.39983340274885465No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATAAGCA460.38317367763431903No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATA440.36651395251978347No Hit
GCCACAACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT440.36651395251978347No Hit
GCCACAACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTATAA430.3581840899625156No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA430.3581840899625156No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA430.3581840899625156No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA420.3498542274052478No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATAAGCA410.34152436484798No Hit
GCCACAACTGGGATTAGATACCCCGACGACTAGAGGAGCCTGTTCTATAA400.33319450229071224No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA390.32486463973344437No Hit
GCCACAACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAT370.3082049146189088No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA360.299875052061641No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAT340.2832153269471054No Hit
GCCACAACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT340.2832153269471054No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA340.2832153269471054No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTATA330.2748854643898376No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC310.25822573927530196No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA310.25822573927530196No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATAAGCAC310.25822573927530196No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT300.24989587671803418No Hit
GCCACAACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTATAAGCAC270.22490628904623072No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATAAGCA250.20824656393169513No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAT230.19158683881715952No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAT220.18325697625989174No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG220.18325697625989174No Hit
GCCACAACTGGGATTAGATACCCCCATCTAGAGGAGCCTGTTCTATAAGC200.16659725114535612No Hit
GCCACAACTGGGATTAGATACCCCGTCTCTTATAACATGACGCTAGAGGA200.16659725114535612No Hit
GCCACAACTGGGATTAGATACCCCATCTCTAGAGGAGCCTGTTCTATAAG190.15826738858808828No Hit
GCCACAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTATAAGCA180.1499375260308205No Hit
GCCACAACTGGGATTAGATACCCCCGAGCCCTAGAGGAGCCTGTTCTATA180.1499375260308205No Hit
GCCACAACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTATAAG180.1499375260308205No Hit
GCCACAACTGGGATTAGATACCCCCGACCAACCTAGAGGAGCCTGTTCTA180.1499375260308205No Hit
GCCACAACTGGGATTAGATACCCCTGACGTGCGGACTAGAGGAGCCTGTT170.1416076634735527No Hit
GCCACAACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTATAAG170.1416076634735527No Hit
GCCACAACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA160.13327780091628488No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT160.13327780091628488No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA160.13327780091628488No Hit
GCCACAACTGGGATTAGATACCCCAATCTAGAGGAGCCTGTTCTATAAGC150.12494793835901709No Hit
GCCACAACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGG150.12494793835901709No Hit
GCCACAACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTATAAG150.12494793835901709No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC150.12494793835901709No Hit
GCCACAACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTATAAGC150.12494793835901709No Hit
GCCACAACTGGGATTAGATACCCCAAAACCTAGAGGAGCCTGTTCTATAA150.12494793835901709No Hit
GCCACAACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTATAAG140.11661807580174927No Hit
GCCACAACTGGGATTAGATACCCCATGTGTATAGAGGAGCCTGTTCTATA140.11661807580174927No Hit
GCCACAACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCC130.10828821324448147No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGG11850.0295.06
CAACTGG11850.0295.05
TGGGATA108.3611236E-4295.09
CTGGGAT11900.0293.760538
ACTGGGA11900.0293.760537
GCCACAA12050.0292.551851
TGGGATT11850.0292.510539
CCACAAC12150.0290.144042
ACAACTG12200.0287.74594
CACAACT12700.0277.578743
CGCCAGA3300.059.00000470-74
TACCCCT2150.059.00000415-19
TACCCCG1550.059.00000415-19
CCCCACG2150.059.00000420-24
AACGCTT257.744478E-659.090-94
AAACTGC2200.059.060-64
ACGCTTA257.744478E-659.090-94
CCCAGTA201.8333267E-459.020-24
CCAAAGG700.059.0100-104
GGGTAGT201.8333267E-459.025-29
Produced by FastQC (version 0.11.5)