FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-159-5-7-5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-159-5-7-5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41143
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1341632.60822011034684No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTATT33818.217679799722918No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG31797.726709282259437No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATTCCTCTGTC14353.4878351116836397No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG14343.485404564567484No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC13523.2860997010427044No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT8622.09513161412634No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTATT6811.6552025861021316No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATTCCTCT6691.6260360207082614No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT5911.4364533456481052No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATTCCTCT5481.331939819653404No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG5441.3222176311887806No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATTCCTC5121.2444401234717934No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAT4791.1642320686386507No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATTCCTC4631.125343314780157No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATTCCTCTG3630.8822886031645722No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATTCCTCTGT3570.8677053204676372No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG3430.8336776608414553No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG3400.826386019492988No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG2710.6586782684782345No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG2630.6392338915489877No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG2530.6149284203874292No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT2090.5079843472765719No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATTCCTC2060.5006927059281044No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG2030.49340106457963684No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG1990.4836788761150135No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATTC1590.38645699146877965No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG1530.37187370877184456No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTATTC1510.3670126145395328No Hit
AAGCGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA1480.35972097319106533No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAT1450.3524293318425978No Hit
AAGCGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTATT1290.31354057798410423No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG1270.3086794837517925No Hit
AAGCGACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA1240.30138784240332495No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT1230.29895729528716913No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG1200.2916656539387016No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTATT1170.28437401259023404No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTATT950.23090197603480545No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT910.22117978757018206No Hit
AAGCGACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTATTCCT860.2090270519894028No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAT830.20173541064093525No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT810.19687431640862357No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT800.19444376929246773No Hit
AAGCGACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTATTCC760.18472158082784434No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTATTC730.1774299394793768No Hit
AAGCGACTGGGATTAGATACCCCATGCTAGAGGAGCCTGTTCTATTCCTC700.17013829813090928No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAT670.16284665678244173No Hit
AAGCGACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTATT620.15069392120166247No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC620.15069392120166247No Hit
AAGCGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTATTCC610.14826337408550663No Hit
AAGCGACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGGT610.14826337408550663No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATTCCTCT600.1458328269693508No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGTGACCTAGAGGAGCCTGT580.1409717327370391No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTATTCCT570.13854118562088324No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG570.13854118562088324No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATTCC540.13124954427241572No Hit
AAGCGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTATTCCT540.13124954427241572No Hit
AAGCGACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTATTCCT540.13124954427241572No Hit
AAGCGACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA530.12881899715625988No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTAT530.12881899715625988No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATTCCTCT510.12395790292394819No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT510.12395790292394819No Hit
AAGCGACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTCTA500.12152735580779232No Hit
AAGCGACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTATTCC480.11666626157548064No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG480.11666626157548064No Hit
AAGCGACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTATT480.11666626157548064No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACAG470.11423571445932479No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG450.10937462022701311No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTATTC440.10694407311085727No Hit
AAGCGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTATTC430.1045135259947014No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG430.1045135259947014No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGG108.439995E-4294.946473
GATGGGA108.439995E-4294.946475
CGGGATT108.439995E-4294.946477
GCGATGG157.1949908E-6294.946473
CGATGGG157.1949908E-6294.946474
CTGGGTT206.118535E-8294.946477
CTGGATT108.439995E-4294.946477
ACTGGGT206.118535E-8294.946476
ACTGGAT108.439995E-4294.946476
CGACTGG40700.0294.584144
GACTGGG40500.0294.582345
CTGGGAT40250.0294.580087
GCGACTG40750.0294.22273
ACTGGGA40300.0294.21466
AGCGACT40850.0293.863432
TGGGATT40200.0293.845928
GGGATTA40300.0292.750859
AAGCGAC41250.0292.08641
ATGGGAT150.0028412933196.630986
CGACGGG150.0028412933196.630984
Produced by FastQC (version 0.11.5)