FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-158-6-7-4-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-158-6-7-4-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39697
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA2009550.6209537244628No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA29417.4086202987631316No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA14883.748394085195355No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGG13033.2823639065924377No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA10632.6777842154318967No Hit
TCGGTCAAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA10512.64755523087387No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA8402.116028919061894No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA8252.07824268836436No Hit
TCGGTCAAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC7831.9724412424112654No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA7751.9522885860392474No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA7191.8112199914351212No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA5811.4635866690178099No Hit
TCGGTCAAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA5071.277174597576643No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA3030.7632818600901832No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA2900.7305337934856538No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA2360.5945033629745321No Hit
TCGGTCAAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAA2080.5239690656724689No Hit
TCGGTCAAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAATAG1920.48366375292843283No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAA1830.4609920145099126No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA1690.42572486585888103No Hit
TCGGTCAAACTGGGATTAGATACCCCACTACGCTTAGCCCTAAACCTCAA1550.39045771720784944No Hit
TCGGTCAAACTGGGATTAGATACCCCAGTAGTCTACGCCCTAAACGATGA1520.3829004710683427No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA1290.3249615839987908No Hit
TCGGTCAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1190.2997707635337683No Hit
TCGGTCAAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1150.2896944353477593No Hit
TCGGTCAAACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACTATAA980.24687004055722095No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA850.21412197395269164No Hit
TCGGTCAAACTGGGATTAGATACCCCTATGCTTAGCCCTAAACTCTAATA840.21160289190618936No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA800.20152656372018035No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGA770.1939693175806736No Hit
TCGGTCAAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC760.19145023553417134No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGTTTAGCCCTAAACCTCAA750.1889311534876691No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAG580.14610675869713077No Hit
TCGGTCAAACTGGGATTAGATACCCCGGTAGTCCTAGCCCTAAACGATGA550.138549512557624No Hit
TCGGTCAAACTGGGATTAGATACCCTGGTAGTCCACGCTGTAAACGTTGA510.12847318437161498No Hit
TCGGTCAAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAATAG500.12595410232511273No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA490.12343502027861047No Hit
TCGGTCAAACTGGGATTAGATACCCCGAGAGTTTTCGGAAGATCGTGGCC430.10832052799959693No Hit
TCGGTAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC420.10580144595309468No Hit
TCGGTCAAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCT410.10328236390659243No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGA108.435799E-4294.982369
AACTGTG108.435799E-4294.982368
TCGGTCC108.435799E-4294.982361
GGTCAAA39150.0294.982363
CGGTCAC108.435799E-4294.982362
CGGTCAA39450.0294.982362
ACTGGGT108.435799E-4294.982369
GTCAACT303.6379788E-12294.982334
TCGGTCA39550.0294.982331
AAACTGG38850.0294.602727
TCAAACT39100.0293.85075
CAAACTG39100.0293.85076
GTCAAAC39400.0293.110634
AACTGGG39100.0292.719068
ACTGGGA39100.0292.341869
CAACTGG351.2732926E-11252.842016
TCAACTG351.2732926E-11252.842015
GGTCAAC351.2732926E-11252.842013
AAACTGT659.858923E-1068.072857
CAGCCCT351.4124453E-859.0709635-39
Produced by FastQC (version 0.11.5)