FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-157-6-7-5-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-157-6-7-5-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40003
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2354058.84558658100643No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG13523.379746519011074No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG11362.839787015973802No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG11092.772292078094143No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG8242.059845511586631No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG8092.0223483238757094No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG7691.9223558233132514No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG6411.6023798215133864No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG6401.599880008999325No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG5781.4448916331275155No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC4771.1924105692073095No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG4671.167412444066695No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTACGCCCTAAACGATGATCG3640.9099317551183662No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATGA3570.8924330675199359No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG3340.8349373796965228No Hit
AAGCGACTGGGATTAGATACCCCCGCAGCAAGCGCATCGCGAACACCGTG1950.4874634402419819No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACAG1770.4424668149888758No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1610.4024698147638927No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG1400.34997375196860236No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTCCTAAACTTCAACAG1350.3374746893982951No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG1300.3249756268279879No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG1220.3049771267154963No Hit
AAGCGACTGGGATTAGATACCCCACTACGCTTAGCCCTAAAGTCGAATAG1120.27997900157488187No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC1080.26997975151863607No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1010.252481063920206No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGATAA970.2424818138639602No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT940.23498237632177585No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCACGCCCTAAACGTTCCTCT900.2249831262655301No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT860.2149838762092843No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAGACCTCAACAG780.19498537609679276No Hit
AAGCGACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACTATAATAG750.1874859385546084No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACA740.18498612604054696No Hit
AAGCGACTGGGATTAGATACCCCAACACTCATCAAGATGGCCAAGTCTGG690.17248706347023973No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT670.16748743844211683No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACAGATCT650.16248781341399396No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG590.14748893832962529No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA560.13998950078744093No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCATAAACACAGATAA550.13748968827337948No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG520.12999025073119516No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCCACGAGACTCTCCGATATCTC520.12999025073119516No Hit
AAGCGACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT470.11749118816088792No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTGTC470.11749118816088792No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTAG460.11499137564682647No Hit
AAGCGACTGGGATTAGATACCCCAAGACAAGAAATTGATTTAAAATATAT460.11499137564682647No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG460.11499137564682647No Hit
AAGCGATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT430.10749193810464215No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCACGCCCTAAACGATGAATG410.10249231307651926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG157.189192E-6294.991274
GCGACGG157.189192E-6294.991273
GCTGGGA157.189192E-6294.991275
GCGCTGG157.189192E-6294.991273
GACTGGA157.189192E-6294.991275
AAGCGCT157.189192E-6294.991271
CGACGGG157.189192E-6294.991274
AGCGCTG157.189192E-6294.991272
AGCGACG157.189192E-6294.991272
CGACTGG39400.0294.991274
GCGACTG39450.0294.991243
GGGATTG108.4353157E-4294.991249
GACTGGG39250.0294.991245
ACTGGGA39250.0294.991246
ACTGGAT108.4353157E-4294.991246
CAGGAGA108.4353157E-4294.99124295
AGCGACT39450.0294.991242
AAGCGAC39750.0294.62021
CTGGGAT39400.0293.86827
TGGGATT39300.0293.865368
Produced by FastQC (version 0.11.5)