FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-156-6-3-8-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-156-6-3-8-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38753
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA1724144.4894588806028No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGG25626.611101076045725No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA21205.47054421593167No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA16844.345470028126855No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA14153.6513302195959025No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA10722.766237452584316No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTG9512.454003561014631No Hit
TGATTGACACTGGGATTAGATACCCCGAAATTAATACGACTCACTAGAGG9252.386911981007922No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA9082.3430444094650738No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA7561.9508167109643124No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA7531.9430753748096923No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA6221.6050370293912728No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC4371.1276546331896886No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA3800.9805692462519031No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATC2620.6760766908368384No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACG2460.6347895646788636No Hit
TGATTGACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAA2380.6141460015998761No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA2270.5857611023662684No Hit
TGATTGACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGC2230.5754393208267747No Hit
TGATTGACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAG2050.528991303899053No Hit
TGATTGACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAG1980.510928186204939No Hit
TGATTGACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCT1950.5031868500503187No Hit
TGATTGACACTGGGATTAGATACCCCCCCCTAGAGGAGCCTGTTCTAGAC1860.4799628415864578No Hit
TGATTGACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGACGT1670.4309343792738627No Hit
TGATTGACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGACGT1610.4154517069646221No Hit
TGATTGACACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTA1570.4051299254251284No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCCAAA1460.3767450261915206No Hit
TGATTGACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAGACG1010.26062498387221633No Hit
TGATTGACACTGGGATTAGATACCGAAATTAATACGACTCACTAGAGGAG980.2528836477175961No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC920.2374009754083555No Hit
TGATTGACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGACG900.23224008463860862No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATC880.22707919386886177No Hit
TGATTGACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGAT760.1961138492503806No Hit
TGATTGACACTGGGATTAGATACCCCACGTCTAGAGGAGCCTGTTCTAGA660.17030939540164633No Hit
TGATTGACACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACTATAA660.17030939540164633No Hit
TGATTGACACTGGGATTAGATACCCCCACGTCTAGAGGAGCCTGTTCTAG620.1599876138621526No Hit
TGATTGACACTGGGATTAGATACCCCCAGAACAGGCTCCTCTAGGAAATT610.15740716847727917No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC580.14966583232265887No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA510.1316027146285449No Hit
TGATTGACACTGGGATTAGATACCCCTCGGCATGTGTCTAGAGGAGCCTG460.11870048770417775No Hit
TGATTGACACTGGGATTAGATACCCCACACTAGAGGAGCCTGTTCTAGAC460.11870048770417775No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA440.11353959693443089No Hit
TGATTGAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC420.10837870616468404No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAGTCGAAT410.1057982607798106No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT108.4343215E-4294.990979
TGATTGC108.4343215E-4294.990971
ACACTGG38100.0294.990977
GATTGAC38550.0294.990972
CATGGGA108.4343215E-4294.990978
CACTGGG37800.0294.600778
TTGACAC38500.0294.224734
GACACTG38350.0294.221746
ATTGACA38650.0293.84613
TGATTGA38800.0293.47041
ACTGGGA37900.0293.434279
TGACACT38650.0292.701265
CACTGGA400.0258.11718
ACTGGAT351.2732926E-11252.84949
ACATGGG150.002839386196.660667
TCAAGCA200.006713383147.49548295
CTCCACA200.006713383147.49548295
GACATGG200.006713383147.495486
CTGGCCC1900.059.0744230-34
TGCCTAG3950.059.0744230-34
Produced by FastQC (version 0.11.5)