FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-155-4-1-3-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-155-4-1-3-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38885
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA547414.077407740774078No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAA480712.362093352192362No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC409010.518194676610518No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTGA24536.308345120226308No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA12093.109168059663109No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGA11042.839141056962839No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT9852.533110453902533No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA9472.4353863957824355No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTGA9102.34023402340234No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTG9092.3376623376623376No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCTG8322.1396425356821394No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGAG6681.717886074321718No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT5421.3938536710813938No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT4851.2472675839012473No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT4661.1984055548411985No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGC3580.9206634949209206No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT3550.9129484377009129No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT3220.8280828082808281No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT2870.738073807380738No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT2550.6557798637006558No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT2470.6352063777806352No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAGC2460.6326346920406327No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA2100.54005400540054No Hit
ATCGGTTACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTA2100.54005400540054No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGCTGA2080.5349106339205348No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT1970.5066220907805066No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCTG1970.5066220907805066No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAG1870.48090523338048086No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1840.47319017616047315No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1780.45776006172045774No Hit
ATCGGTTACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAGC1700.4371865758004372No Hit
ATCGGTTACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT1600.41146971840041147No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGCTG1480.3806094895203806No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT1460.3754661180403755No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAG1430.3677510608203678No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGCT1350.3471775749003472No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAG1320.33946251768033947No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAG1290.33174746046033177No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGC1190.30603060306030605No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCTAGAGGAGCCTGTTCTAGC1170.3008872315803009No Hit
ATCGGTTACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGCT1130.2906004886202906No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT1110.2854571171402855No Hit
ATCGGTTACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGCTGA1110.2854571171402855No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1100.2828854314002829No Hit
ATCGGTTACTGGGATTAGATACCCCACGAACTAGAGGAGCCTGTTCTAGC1070.2751703741802752No Hit
ATCGGTTACTGGGATTAGATACCCCCCGAGACCTAGAGGAGCCTGTTCTA940.24173845956024176No Hit
ATCGGTTACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGCTG920.23659508808023658No Hit
ATCGGTTACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAG880.2263083451202263No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT850.2185932879002186No Hit
ATCGGTTACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA850.2185932879002186No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT780.2005914877202006No Hit
ATCGGTTACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTA760.19544811624019545No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA760.19544811624019545No Hit
ATCGGTTACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGCT750.19287643050019287No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGCT740.1903047447601903No Hit
ATCGGTTACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC720.18516137328018514No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC710.1825896875401826No Hit
ATCGGTTACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTAG710.1825896875401826No Hit
ATCGGTTACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGCT670.1723029445801723No Hit
ATCGGTTACTGGGATTAGATACCCCGACGAACCTAGAGGAGCCTGTTCTA650.16715957310016716No Hit
ATCGGTTACTGGGATTAGATACCCCCACGGACCTAGAGGAGCCTGTTCTA650.16715957310016716No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGCTAGAGGAGCCTGTTCTAGCT640.16458788736016458No Hit
ATCGGTTACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGCTGA630.16201620162016203No Hit
ATCGGTTACTGGGATTAGATACCCCTACCCTAGAGGAGCCTGTTCTAGCT630.16201620162016203No Hit
ATCGGTTACTGGGATTAGATACCCCTTTCCGAAGTGCAGCTGTTGGAGAC620.15944451588015943No Hit
ATCGGTTACTGGGATTAGATACCCCTGTGCTAGAGGAGCCTGTTCTAGCT620.15944451588015943No Hit
ATCGGTTACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTC610.15687283014015688No Hit
ATCGGTTACTGGGATTAGATACCCCTCTCGAGAGCTAGAGGAGCCTGTTC590.15172945866015172No Hit
ATCGGTTACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGC570.1465860871801466No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT570.1465860871801466No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT550.14144271570014144No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGTGCGGACTAGAGGAGCCTGT550.14144271570014144No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTAGC540.13887102996013886No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGCT530.13629934422013631No Hit
ATCGGTTACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGCTGA520.1337276584801337No Hit
ATCGGTTACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGCT520.1337276584801337No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGATCTAGAGGAGCCTGTTCTAG520.1337276584801337No Hit
ATCGGTTACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCC510.13115597274013116No Hit
ATCGGTTACTGGGATTAGATACCCCGACCCACCTAGAGGAGCCTGTTCTA500.12858428700012858No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGAACTAGAGGAGCCTGTTCTA500.12858428700012858No Hit
ATCGGTTACTGGGATTAGATACCCCTATAAGAGGAGCCTGTTCTAGCTGA490.126012601260126No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGCTGA490.126012601260126No Hit
ATCGGTTACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGC480.12344091552012343No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGCTGA470.12086922978012088No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT460.11829754404011829No Hit
ATCGGTTACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTA450.11572585830011572No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC440.11315417256011315No Hit
ATCGGTTACTGGGATTAGATACCCCCACAGAGAGCTAGAGGAGCCTGTTC440.11315417256011315No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC430.11058248682011058No Hit
ATCGGTTACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGCTGA390.1002957438601003No Hit
ATCGGTTACTGGGATTAGATACCCCCCCAAATGCACCTAGAGGAGCCTGT390.1002957438601003No Hit
ATCGGTTACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGCTGAG390.1002957438601003No Hit
ATCGGTTACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGCTGA390.1002957438601003No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG108.4336306E-4295.04
CGGTTAT108.4336306E-4295.03
TTACGGG206.108712E-8295.06
CGGTACT108.4336306E-4295.03
GGTTACT38050.0295.04
CCGGGAT157.1867507E-6294.999979
GTTACCG157.1867507E-6294.999975
TTACCGG157.1867507E-6294.999976
TACCGGG157.1867507E-6294.999977
ATCGGTT38700.0294.618871
TCGGTTA38600.0294.617862
CGGTTAC38500.0294.616883
TTACTGG38000.0294.611826
ACTGGGA37750.0294.609288
CTGGGAT37850.0294.22069
TACTGGG37850.0293.44127
GTTACTG38300.0292.30425
TACTGGA351.2732926E-11252.857137
GGTTACG301.553417E-9245.833314
ACTGGAT301.553417E-9245.833318
Produced by FastQC (version 0.11.5)