FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-154-6-7-5-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-154-6-7-5-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36941
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1948552.74627108091281No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG15004.060528951571425No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG12593.408137300018949No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG10202.761159687068569No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG10002.7070193010476165No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG9272.5094068920711403No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG8392.2711891935789503No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG7231.9571749546574269No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC7041.905741587937522No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG6771.8326520668092365No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG5481.4834465769740937No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG5381.4563763839636177No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT4221.1423621450420942No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG3590.9718199290760944No Hit
AAGCGACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAATAGT3360.9095584851519992No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG3070.8310549254216183No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG2540.6875829024660945No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA2220.6009582848325709No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG1910.5170406865000947No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT1550.4195879916623806No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACGG1250.33837741263095206No Hit
AAGCGACTGGGATTAGATACCCCACTACGCTTAGCCCTAAAGTCGAATAG1150.31130721962047586No Hit
AAGCGACTGGGATTAGATACCCCCGCAGCAAGCGCATCGCGAACACCGTG1070.289651065212095No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGCTG1040.2815300073089521No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1030.2788229880079045No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATAG980.2652878915026664No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC950.2571668335995236No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCCTAGCCGTAAACTTTGATAG920.24904577569638073No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT830.22468260198695217No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCACGCCCTAAACGATCTTCT810.21926856338485692No Hit
AAGCGACTGGGATTAGATACCCCAATGTGGGCAAACGTCGATGCGTTGTT760.20573346687961885No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGG760.20573346687961885No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACAG710.19219837037438078No Hit
AAGCGACTGGGATTAGATACCCCAACACTCATCAAGATGGCCAAGTCTGG680.18407731247123793No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT670.1813702931701903No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGGTGA590.15971413876180937No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTAAGCCCTAAACCTCAACAG580.15700711946076173No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCCACGAGACTCTCCGATATCTC560.15159308085866652No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTACGCCCTAAACGATGATCG550.1488860615576189No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCCAAATAG460.12452288784819036No Hit
AAGCGACTGGGATTAGATACCCCTGGAATTATAAAGCCAGCCTATGAGTT450.12181586854714274No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG440.11910884924609512No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG440.11910884924609512No Hit
AAGCGACTGGGATTAGATACCCCAAGACAAGAAATTGATTTAAAATATAT440.11910884924609512No Hit
AAGCGACTGGGATTAGATACCCCATAAATGATTATCGAATGTACAAGGTT380.10286673343980943No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG370.1001597141387618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGGG36400.0294.971565
ACTGGGA36200.0294.971566
GATGGGA108.434353E-4294.971535
ATGGGAT108.434353E-4294.971536
GCGACTT108.434353E-4294.971533
GCGACTG36550.0294.971533
CTGGGTT157.1865925E-6294.971537
ACTTGGA108.434353E-4294.971536
GCGACGG108.434353E-4294.971533
GCGATGG108.434353E-4294.971533
GACGGGA108.434353E-4294.971535
CTTGGAT108.434353E-4294.971537
ACGGGAT108.434353E-4294.971536
GGGATTG157.1865925E-6294.971539
GACTGGA157.1865925E-6294.971535
CGACGGG108.434353E-4294.971534
CGATGGG108.434353E-4294.971534
TTGGATT108.434353E-4294.971538
TGGGATA108.434353E-4294.971538
ACTGGGT157.1865925E-6294.971536
Produced by FastQC (version 0.11.5)