FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-153-6-7-5-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-153-6-7-5-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36470
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2004354.95749931450508No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG22526.17493830545654No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG20385.588154647655608No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG7422.034548944337812No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG6741.8480943241020016No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC6671.8289004661365504No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG5961.63421990677269No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG5431.488894982177132No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG5001.3709898546750754No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG4091.1214697011242118No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG3570.9788867562380038No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG3060.8390457910611462No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT2950.8088840142582945No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG2760.7567863997806415No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT2700.7403345215245407No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG2420.6635590896627365No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG2290.6279133534411846No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG2270.6224293940224842No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTACGCCCTAAACGATGATCG2060.5648478201261311No Hit
AAGCGACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATAG1560.42774883465862346No Hit
AAGCGACTGGGATTAGATACCCCCCTATGCTTAGACCTAAACTCTAATAG1540.42226487523992323No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACGG1370.37565122018097064No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT1310.3591993419248698No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT1190.32629558541266795No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG1170.3208116259939677No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATAC1150.31532766657526734No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAATAGGAT1120.3071017274472169No Hit
AAGCGACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACAG1110.3043597477378667No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATCT1100.3016177680285166No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1060.29064984919111597No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATAGATAA930.25500411296956405No Hit
AAGCGACTGGGATTAGATACCCCACAGCTAAAGATATTGGGATTAGAGAC850.2330682752947628No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC780.21387441732931173No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT700.19193857965451055No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACAG690.1891965999451604No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA690.1891965999451604No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAATAGGAT600.16451878256100905No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGGTAC570.15629284343295857No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAATAGGATTCT550.1508088840142583No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG520.14258294488620785No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCACTAAACCTCAACAG480.13161502604880723No Hit
AAGCGACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT390.10693720866465586No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG390.10693720866465586No Hit
AAGCGACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATAA390.10693720866465586No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATAG390.10693720866465586No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG370.10145324924595558No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGATT350.0295.000037
TGGATTA350.0295.000038
GACTGGA350.0295.000035
ACTGGAT350.0295.000036
GGGTTAG108.431493E-4295.09
GCGACTG36150.0295.03
GGGATTG108.431493E-4295.09
TGGGTTA108.431493E-4295.08
ACTGGGT108.431493E-4295.06
ACTGGGA35650.0295.06
AGCGACT36200.0295.02
CGACTGG36150.0295.04
GACTGGG35800.0294.999975
CTGGGAT35800.0294.175967
AAGCGAC36400.0293.784331
TGGGATT35900.0293.356578
GGGATTA35900.0292.534829
CTGGGTT150.0028384365196.666677
CCACTGA150.0028384365196.66667295
TCCAAAA200.006711141147.5295
Produced by FastQC (version 0.11.5)