FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-152-6-7-5-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-152-6-7-5-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36089
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2137359.223031948793256No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG19615.433788689074233No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG8852.452270775028402No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG8802.4384161378813487No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG8352.313724403557871No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT7792.158552467510876No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG6571.8204993211227798No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG6251.73182964338164No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG6071.6819529496522485No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG5831.6154506913463935No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC5341.479675247305273No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG4251.1776441574995151No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG3751.0390977860289838No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG3450.9559699631466652No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA2460.6816481476350135No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTTATGC1920.5320180664468398No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG1670.46274488071157416No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1460.404555404693951No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACAG1310.3629914932527917No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCCAAATAG1290.3574496383939705No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG1090.302031089805758No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACAG990.27432181551165175No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT970.2687799606528305No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG840.2327579040704924No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT770.21336141206461803No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC690.19119399262933304No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATGA660.18288121034110116No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATTGACAA650.18011028291169054No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAACT590.16348471833522682No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG580.16071379090581617No Hit
AAGCGACTGGGATTAGATACCTCACTATGCTTAGCCCTAAACCTCAACAG570.15794286347640554No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG500.13854637147053117No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG480.13300451661170995No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGC480.13300451661170995No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCACGCCCTAAACGATGAATG400.11083709717642495No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCTGAG390.10806616974701432No Hit
AAGCGATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT370.10252431488819308No Hit
AAGCGACTGGGATTAGATACCCCAGAAATGGCCGTTAAAATGCGGGATGA370.10252431488819308No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGGA108.4311096E-4295.05
ATGGGAT108.4311096E-4295.06
GCGACTG35600.0295.03
GCGACGG108.4311096E-4295.03
GCGATGG108.4311096E-4295.03
GACGGGA108.4311096E-4295.05
CTGGATT255.2023097E-10295.07
CGGGATT108.4311096E-4295.07
TGGATTA255.2023097E-10295.08
ACGGGAT108.4311096E-4295.06
GACTGTG108.4311096E-4295.05
GTGATTA108.4311096E-4295.09
GGGATTG255.2023097E-10295.09
GACTGGG35150.0295.05
GACTGGA303.6379788E-12295.05
AAGCGAT157.1824506E-6295.01
CGACGGG108.4311096E-4295.04
ACTGGGA35150.0295.06
ACTGGAT255.2023097E-10295.06
AGCGATG108.4311096E-4295.02
Produced by FastQC (version 0.11.5)