FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-151-5-1-3-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-151-5-1-3-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35418
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1075530.36591563611723No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT26577.501835225026822No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA20595.8134282003501045No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATTCCTCTG15984.51183014286521No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATTCCT11573.266700547744085No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT11503.2469365859167656No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT10462.9533005816251623No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA9122.5749618837879047No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT5321.5020610988762777No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATTCCTCT4711.3298322886667795No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC4701.3270088655485912No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATTCCT4551.2846575187757636No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATTCC4191.183014286520978No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATTCCTC3971.1208989779208312No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT3771.0644305155570613No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC3571.0079620531932916No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT3150.8893782822293748No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATTCC3030.855497204811113No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC2660.7510305494381389No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATTCC2550.7199728951380654No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2510.7086792026653115No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC2270.6409170478287876No Hit
ATCGGTTACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAT2160.6098593935287142No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT2030.5731548929922639No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT1970.5562143542831329No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA1720.48562877632842055No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT1650.4658648145011012No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATT1650.4658648145011012No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1530.4319837370828392No Hit
ATCGGTTACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGT1470.4150431983737083No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT1460.4122197752555198No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATTCCT1460.4122197752555198No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1460.4122197752555198No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT1450.40939635213733133No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1410.3981026596645773No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT1230.3472810435371845No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT1100.31057654300073406No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT1100.31057654300073406No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAT1100.31057654300073406No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATTCC1060.29928285052798015No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG940.26540177310971824No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAT930.26257834999152974No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATTCCT850.23999096504602177No Hit
ATCGGTTACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTATTC730.2061098876277599No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAT720.20328646450957139No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG710.20046304139138293No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT700.19763961827319443No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTATTC680.19199277203681742No Hit
ATCGGTTACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT650.18352250268225198No Hit
ATCGGTTACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA640.18069907956406345No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT610.172228810209498No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTT570.16093511773674404No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA570.16093511773674404No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTATT540.15246484838217855No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC540.15246484838217855No Hit
ATCGGTTACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATTCCT530.14964142526399007No Hit
ATCGGTTACTGGGATTAGATACCCCACGTCTAGAGGAGCCTGTTCTATTC530.14964142526399007No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATAT490.1383477327912361No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAT490.1383477327912361No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATTCCTC480.1355243096730476No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC460.12987746343667061No Hit
ATCGGTTACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAT450.12705404031848214No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTTCCTCTGTCTCTTATA430.12140719408210514No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT430.12140719408210514No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTCTCATACCATCTGAGCTAGAG430.12140719408210514No Hit
ATCGGTTACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA430.12140719408210514No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT400.11293692472753966No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAAT400.11293692472753966No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTATTC390.11011350160935118No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTATTC390.11011350160935118No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGTCTAGAGGAGCCTGTTCTAT370.1044666553729742No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAC370.1044666553729742No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAAC360.10164323225478569No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTG108.4304676E-4295.02
TCGGTTA35100.0295.02
GGTTACT34900.0295.04
ATCGGTT35250.0295.01
CGGGATT157.1813556E-6294.999979
ACGGGAT157.1813556E-6294.999978
CGGTTAC35100.0294.579773
ACTGGGA34550.0294.57318
CTGGGAT34600.0294.14749
TACTGGG34550.0294.146157
GTTACTG34950.0293.733925
TTACTGG34850.0293.730266
TACTGGA301.549779E-9245.833317
CTGGATT251.856697E-7236.09
GGTTACG202.2635531E-5221.254
GTTACGG202.2635531E-5221.255
TTACGGG202.2635531E-5221.256
GGTTGTG150.002838092196.666664
CGGTTGT150.002838092196.666663
GTTGTGT150.002838092196.666665
Produced by FastQC (version 0.11.5)