FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-150-6-7-5-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-150-6-7-5-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34099
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2077660.92847297574709No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG15204.457608727528666No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG13674.0089152174550575No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG9392.753746444177249No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG6771.985395466142702No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG5901.730256019238101No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC4831.4164638259186486No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG4311.2639666852400362No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG4281.2551687732778087No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG3911.1466611924103345No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAGCAG2710.7947447139212295No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG2660.7800815273175167No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT2370.6950350450159829No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACAG2280.6686413091293No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG2260.6627760344878149No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG2010.5894601014692513No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT1660.4868177952432623No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG1340.39297340097950084No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG1300.3812428516965307No Hit
AAGCGACTGGGATTAGATACCCCACCATGCCTAGCCGTAAACCTTGATAG1190.34898384116836273No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCCAAATAG1190.34898384116836273No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1120.3284553799231649No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATGA980.2873984574327693No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATAG940.27566790814979913No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG920.269802633508314No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATAG860.25220680958385877No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT850.2492741722631162No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG790.23167834833866097No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG790.23167834833866097No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAAC670.19648670048975042No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCCTG650.19062142584826536No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT570.167160327282325No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG560.16422768996158246No Hit
AAGCGACTGGGATTAGATACCCCCGCAGCAAGCGCATCGCGAACACCGTG540.15836241532009737No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAACTTCGC510.14956450335786975No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC500.1466318660371272No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG460.134901316754157No Hit
AAGCGACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT430.12610340479192939No Hit
AAGCGATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT420.12317076747118685No Hit
AAGCGACTGGGATTAGATACCCCAAAGTTATTTAGCTGAAGAAATTGACT420.12317076747118685No Hit
AAGCGACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACTATAATAG400.11730549282970176No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG370.10850758086747411No Hit
AAGCGACTGGGATTAGATACCCCGGTGGCCCCAAAGGGAGGGGTAGAGGG370.10850758086747411No Hit
AAGCGACTGGGATTAGATACCCCACAGCCCCGGCTGCAAGAGGTCGCGGA350.10264230622598902No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG108.431066E-4294.97659
CTGGGTT108.431066E-4294.97657
GCGACGG157.18123E-6294.97653
CTGGATT157.18123E-6294.97657
CGGGATT157.18123E-6294.97657
TGGATTA157.18123E-6294.97658
GACTGGG33600.0294.97655
CGACGGG157.18123E-6294.97654
ACTGGGT108.431066E-4294.97656
AGCGACT33750.0294.97652
AGCGACG157.18123E-6294.97652
CGACTGG33750.0294.97654
AAGCGAC33950.0294.542081
GCGACTG33800.0294.540163
CTGGGAT33500.0294.536257
ACTGGGA33500.0294.536256
TGGGATT33600.0293.659678
GGGATTA33700.0292.35069
GACGGGA202.2635146E-5221.232385
ACGGGAT202.2635146E-5221.232386
Produced by FastQC (version 0.11.5)