FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-15-4-4-1-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-15-4-4-1-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11781
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG233819.84551396316102No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAAC141512.010864952041423No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCCTG8957.596978185213479No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTTCGC5714.846787199728377No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG3202.7162380103556574No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAACTTCG2872.43612596553773No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAAC2702.291825821237586No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAACTTCGCC2081.7655547067311774No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCGCCT1921.6297428062133943No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1911.621254562431033No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC1711.4514896867838043No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG1661.4090484678719972No Hit
GGTACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAACTTC1641.3920719803072743No Hit
GGTACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAACT1481.2562600797894916No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTTCGC1401.1883541295306002No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAA1351.145912910618793No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1271.0780069603599016No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACTTCG1271.0780069603599016No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1231.0440539852304558No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAA1070.9082420847126729No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAAC1040.8827773533655887No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAACTTCG1030.8742891095832271No Hit
GGTACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAACTTCGC960.8148714031066971No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAACT940.7978949155419743No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAA690.5856888209829386No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAAC680.5772005772005772No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG640.5432476020711315No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAACTT630.53475935828877No Hit
GGTACACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTAAC630.53475935828877No Hit
GGTACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAACTTC590.5008063831593244No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG580.49231813937696295No Hit
GGTACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAACTTC580.49231813937696295No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAACT570.48382989559460143No Hit
GGTACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA560.4753416518122401No Hit
GGTACACTGGGATTAGATACCCCTGACCTGCTAGAGGAGCCTGTTCTAAC540.4583651642475172No Hit
GGTACACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAACT540.4583651642475172No Hit
GGTACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAACTTCGC520.44138867668279436No Hit
GGTACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAACTT450.38197097020626436No Hit
GGTACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAACTTCGC450.38197097020626436No Hit
GGTACACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTCTA380.3225532637297343No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAACTTCGC370.3140650199473729No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAACTTCG340.2886002886002886No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG340.2886002886002886No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG330.2801120448179272No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAA330.2801120448179272No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG320.27162380103556577No Hit
GGTACACTGGGATTAGATACCCCTGACGTGCGGACTAGAGGAGCCTGTTC290.24615906968848147No Hit
GGTACACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTAACTT280.23767082590612004No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG270.2291825821237586No Hit
GGTACACTGGGATTAGATACCCCCGACGCTAGAGGAGCCTGTTCTAACTT270.2291825821237586No Hit
GGTACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG260.22069433834139718No Hit
GGTACACTGGGATTAGATACCCCCGTCCTAGAGGAGCCTGTTCTAACTTC250.21220609455903572No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG250.21220609455903572No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT250.21220609455903572No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG250.21220609455903572No Hit
GGTACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAACTTC240.2037178507766743No Hit
GGTACACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAACT230.19522960699431288No Hit
GGTACACTGGGATTAGATACCCCAATGTCAGACTAGAGGAGCCTGTTCTA210.17825311942959002No Hit
GGTACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAAC210.17825311942959002No Hit
GGTACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAACT200.16976487564722859No Hit
GGTACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAACTTCGC200.16976487564722859No Hit
GGTACACTGGGATTAGATACCCCTACCCTAGAGGAGCCTGTTCTAACTTC190.16127663186486715No Hit
GGTACACTGGGATTAGATACCCCAAACTAGAGGAGCCTGTTCTAACTTCG180.15278838808250572No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG180.15278838808250572No Hit
GGTACACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTGTT180.15278838808250572No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAA170.1443001443001443No Hit
GGTACACTGGGATTAGATACCCCAAGCTAGAGGAGCCTGTTCTAACTTCG170.1443001443001443No Hit
GGTACACTGGGATTAGATACCCCGACAATCTAGAGGAGCCTGTTCTAACT170.1443001443001443No Hit
GGTACACTGGGATTAGATACCCCCCGACCTAGAGGAGCCTGTTCTAACTT170.1443001443001443No Hit
GGTACACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAACTTC170.1443001443001443No Hit
GGTACACTGGGATTAGATACCCCACGAACCTAGAGGAGCCTGTTCTAACT160.13581190051778289No Hit
GGTACACTGGGATTAGATACCCCAGATCTAGAGGAGCCTGTTCTAACTTC160.13581190051778289No Hit
GGTACACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTGT160.13581190051778289No Hit
GGTACACTGGGATTAGATACCCCGGAACTAGAGGAGCCTGTTCTAACTTC150.12732365673542145No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAACT150.12732365673542145No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT140.11883541295306002No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT140.11883541295306002No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC130.11034716917069859No Hit
GGTACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA120.10185892538833714No Hit
GGTACACTGGGATTAGATACCTAGAGGAGCCTGTTCTAACTTCGCCTGTC120.10185892538833714No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG120.10185892538833714No Hit
GGTACACTGGGATTAGATACCCCCGTCAGAGGAGCCTGTTCTAACTTCGC120.10185892538833714No Hit
GGTACACTGGGATTAGATACCCCAGACGCTGCTAGAGGAGCCTGTTCTAA120.10185892538833714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT11450.0295.07
CACTGGG11450.0295.05
ACTGGGA11450.0295.06
GGTACAC11600.0295.01
ACACTGG11500.0294.999974
TGGGATT11450.0293.711768
GGGATTA11500.0292.434759
TACACTG11750.0288.72343
GTACACT11850.0287.531652
TAAGTAA200.0066536483147.5295
GCCCCAA150.00435987759.000004115-119
GGAGATC257.742468E-659.00000425-29
AAACTTT150.00435987759.00000435-39
AAACCGC150.00435987759.00000460-64
ACTTGGT150.00435987759.000004105-109
CCGCTCG150.00435987759.00000465-69
TTTTTTT150.00435987759.000004225-229
ACCGCTC150.00435987759.00000460-64
TAGCCGT600.059.00000430-34
AGGGCCT257.742468E-659.00000430-34
Produced by FastQC (version 0.11.5)