FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-149-4-7-5-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-149-4-7-5-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34971
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGA532015.212604729633124No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG444712.716250607646334No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGCTG31859.107546252609305No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACGTGG20165.7647765291241315No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGA20085.741900431786337No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTGGCT10432.9824711904149153No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG9162.6193131451774327No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGACGTG8502.4305853421406307No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGAC5731.6385004718195075No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGA5511.5755912041405737No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG5281.5098224242944154No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC5001.4297560836121357No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACGTG4761.3611277915987532No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGACGTGG4191.1981355980669697No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG4001.1438048668897087No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT3651.043721941036859No Hit
AAGCGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGAC3460.9893912098595979No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGACGTGG3350.9579365760201309No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT2880.8235395041605902No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGAC2770.7920848703211232No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAGAC2680.7663492608161048No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGACGTGG2400.6862829201338252No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG2090.5976380429498728No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGACG2050.5861999942809757No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG2000.5719024334448544No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGACGTG1870.5347287752709388No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGACGTG1840.5261502387692659No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGACGTGG1830.5232907266020417No Hit
AAGCGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGACGT1810.5175717022675931No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGACGT1670.47753853192645335No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT1630.4661004832575563No Hit
AAGCGACTGGGATTAGATACCCCGCACGGACCTAGAGGAGCCTGTTCTAG1580.45180292242143494No Hit
AAGCGACTGGGATTAGATACCCCTCCTAGAGGAGCCTGTTCTAGACGTGG1530.4375053615853135No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGCCGTAAACGATGGATA1530.4375053615853135No Hit
AAGCGACTGGGATTAGATACCCCCACGGGACCTAGAGGAGCCTGTTCTAG1510.43178633725086496No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGA1470.42034828858196793No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGACGTGG1420.40605072774584655No Hit
AAGCGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA1380.39461267907694947No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGACGTGG1290.36887706957193106No Hit
AAGCGACTGGGATTAGATACCCCGCCCTAGAGGAGCCTGTTCTAGACGTG1240.3545795087358097No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAG1210.34600097223413684No Hit
AAGCGACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTAGACGTG1170.3345629235652398No Hit
AAGCGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC1170.3345629235652398No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCTA1040.2973892653913242No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGACGTGGC1040.2973892653913242No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG1010.2888107288896514No Hit
AAGCGACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTAGACG990.2830917045552029No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG950.2716536558863058No Hit
AAGCGACTGGGATTAGATACCCCTGCTGACTAGAGGAGCCTGTTCTAGAC940.26879414371908156No Hit
AAGCGACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAGA940.26879414371908156No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG910.2602156072174087No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCTAGAGGAGCCTGTTCTAGACG880.25163707071573593No Hit
AAGCGACTGGGATTAGATACCCCTATAAGAGGAGCCTGTTCTAGACGTGG870.24877755854851163No Hit
AAGCGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGAC840.2401990220468388No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAGA830.23733950987961452No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGACGT790.22590146121071747No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG790.22590146121071747No Hit
AAGCGACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAGAC760.21732292470904463No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGAC750.21446341254182036No Hit
AAGCGACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAGACG740.21160390037459612No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACTAGAGGAGCCTGTTCTAGA730.20874438820737184No Hit
AAGCGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAGACG720.20588487604014755No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG700.200165851705699No Hit
AAGCGACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTAGA690.19730633953847473No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG690.19730633953847473No Hit
AAGCGACTGGGATTAGATACCCCATGTGTATAGAGGAGCCTGTTCTAGAC670.1915873152040262No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG650.18586829086957765No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGACGTGG650.18586829086957765No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG630.1801492665351291No Hit
AAGCGACTGGGATTAGATACCCCGAGTCTAGAGGAGCCTGTTCTAGACGT620.17728975436790484No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT580.16585170569900776No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG560.16013268136455922No Hit
AAGCGACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTAGACGTGGC530.1515541448628864No Hit
AAGCGACTGGGATTAGATACCCCATGTGCTAGAGGAGCCTGTTCTAGACG530.1515541448628864No Hit
AAGCGACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCCT530.1515541448628864No Hit
AAGCGACTGGGATTAGATACCCCCGTCTAGAGGAGCCTGTTCTAGACGTG530.1515541448628864No Hit
AAGCGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAGA520.1486946326956621No Hit
AAGCGACTGGGATTAGATACCCCGAGCCACGAGACTAGAGGAGCCTGTTC500.1429756083612136No Hit
AAGCGACTGGGATTAGATACCCCAAGCCCACTAGACCTAGAGGAGCCTGT500.1429756083612136No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG470.13439707185954078No Hit
AAGCGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG460.13153755969231648No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG350.1000829258528495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGG34800.0294.576144
GACTGGG34650.0294.57435
ACTGGGA34650.0294.57436
GCGACTG34850.0294.15353
CTGGGAT34700.0294.149847
TGGGATT34550.0294.146158
AAGCGAC35000.0293.735721
AGCGACT34900.0293.73212
GGGATTA34650.0293.297249
TGGATTA202.2633074E-5221.249988
TGGGATA202.2633074E-5221.249988
GACTGGA202.2633074E-5221.249985
GGGATAG150.002837938196.666669
CTGGATT255.5107805E-5177.07
ACTGGAT301.1396404E-4147.499986
GATACTT650.059.00000445-49
AGTCCCT650.059.00000425-29
CCAGGGA1400.059.00000470-74
GGCCCTA700.059.00000430-34
TGGCCCT700.059.00000430-34
Produced by FastQC (version 0.11.5)