FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-148-5-7-5-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-148-5-7-5-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34125
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1174034.4029304029304No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG24137.071062271062272No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACC22446.5758241758241756No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCTAGCTGTC12233.5838827838827836No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC10403.0476190476190474No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG9952.9157509157509156No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCTAGCT9362.742857142857143No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT5981.7523809523809524No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG5071.4857142857142858No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCTAGCTG4031.180952380952381No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCTAGCT3981.1663003663003662No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTACC3911.1457875457875457No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCTAGCTGT3471.0168498168498168No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCTAGC3220.9435897435897437No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT3050.8937728937728938No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG3010.882051282051282No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG2990.8761904761904762No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAC2560.7501831501831502No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG2540.7443223443223443No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG2460.7208791208791209No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCTAGC2450.717948717948718No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT2320.6798534798534799No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCTAGC2060.6036630036630037No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG1950.5714285714285714No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAC1910.5597069597069597No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACCTA1700.4981684981684982No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG1670.48937728937728936No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT1610.47179487179487184No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG1440.4219780219780219No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG1410.4131868131868132No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTACC1400.41025641025641024No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTACC1360.39853479853479856No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAC1250.3663003663003663No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACCTAGCT1230.36043956043956044No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCTAGCT1150.336996336996337No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACCT1120.3282051282051282No Hit
AAGCGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTACCTA1060.31062271062271063No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG1050.3076923076923077No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT1030.30183150183150187No Hit
AAGCGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTACC1030.30183150183150187No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTACCTAG1020.2989010989010989No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCACGAGACCTAGAGGAGCCTGT970.28424908424908424No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACCTAG880.2578754578754579No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTACCT850.2490842490842491No Hit
AAGCGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG790.2315018315018315No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACCTAGCT780.2285714285714286No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAC730.21391941391941394No Hit
AAGCGACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTACCTAGC710.20805860805860804No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG710.20805860805860804No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACCT710.20805860805860804No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCTAGC710.20805860805860804No Hit
AAGCGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTACC710.20805860805860804No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT690.2021978021978022No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCTAGCT660.19340659340659339No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT650.19047619047619047No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACCTAGCT580.16996336996336997No Hit
AAGCGACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTCTA580.16996336996336997No Hit
AAGCGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTACCT570.16703296703296705No Hit
AAGCGACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA540.15824175824175826No Hit
AAGCGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC540.15824175824175826No Hit
AAGCGACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTACCTAGC540.15824175824175826No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAC510.14945054945054945No Hit
AAGCGACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTACCTAGCTG490.1435897435897436No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACCTAG480.14065934065934066No Hit
AAGCGACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCTG480.14065934065934066No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCTAGCT470.13772893772893774No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA460.1347985347985348No Hit
AAGCGACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA450.13186813186813187No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG450.13186813186813187No Hit
AAGCGACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTACCTA440.12893772893772895No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACAG430.126007326007326No Hit
AAGCGACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCCT420.12307692307692308No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACCT420.12307692307692308No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG420.12307692307692308No Hit
AAGCGACTGGGATTAGATACCCCACCAGACCTAGAGGAGCCTGTTCTACC410.12014652014652014No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT370.10842490842490843No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTACCT370.10842490842490843No Hit
AAGCGACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTACCTA370.10842490842490843No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATGA360.10549450549450548No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG350.10256410256410256No Hit
AAGCGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACCTAG350.10256410256410256No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGG206.1010724E-8294.96923
CTTGGAT157.1820323E-6294.96927
CGGGATT206.1010724E-8294.96927
GACTGGG33400.0294.96925
CGACGGG206.1010724E-8294.96924
AGCGACT33700.0294.96922
GCGACTG33600.0294.530243
CGACTGG33600.0294.530244
ACTGGGA33400.0294.527626
AAGCGAC34050.0293.669771
CTGGGAT33550.0293.21087
TGGGATT33600.0292.774488
GGGATTA33600.0291.89669
GACGGGA251.8566789E-7235.975365
ACGGGAT251.8566789E-7235.975366
ACTTGGA202.263767E-5221.226916
TTGGATT202.263767E-5221.226918
GATGGGA150.0028385224196.646155
ATGGGAT150.0028385224196.646156
CGATGGG150.0028385224196.646154
Produced by FastQC (version 0.11.5)