FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-147-6-4-6-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-147-6-4-6-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34156
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA1384740.54046141234336No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGAC19015.565640004684389No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA16174.734160908771519No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA11413.3405551001288205No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG9372.7432954678533785No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA7792.2807120271694576No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCATAAACATTGACA6401.8737557090994263No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA6191.8122730998946013No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA5931.7361517742124368No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA5611.6424639887574657No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA5051.4785103642112658No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA4831.4141000117109732No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA4321.2647851036421127No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCTCTAAACTCCAATA4211.2325799273919662No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA4121.2062302377327554No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT3591.0510598430729594No Hit
GCCACAACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAATAG3300.9661552875043916No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA3220.9427333411406489No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA3120.9134559081859702No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA2880.8431900690947418No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATTG2810.8226958660264669No Hit
GCCACAACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTAGA2610.7641410001171097No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGATTG2410.7055861342077527No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAGTCTTGATA2260.6616699847757349No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGATTGA2170.6353202951165242No Hit
GCCACAACTGGGATTAGATACCCCACTATTCTTAGCCCTAAACCTCAACA2140.6265370652301206No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA2130.6236093219346528No Hit
GCCACAACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACCTCAACA2120.6206815786391849No Hit
GCCACAACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGATTGAG2060.6031151188663777No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGA2000.5855486590935707No Hit
GCCACAACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAGAT1940.5679821993207635No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAGA1920.5621267127298278No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG1900.5562712261388922No Hit
GCCACAACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTTC1870.5474879962524886No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1680.49186087363859937No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCTATAAACTCAAATA1630.4772221571612601No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAG1530.44794472420658155No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGATTGA1470.43037826443377447No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGATTGAG1310.3835343717062888No Hit
GCCACAACTGGGATTAGATACCCCATGCTAGAGGAGCCTGTTCTAGATTG1230.36011242534254595No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGATTGAG1140.3337627356833353No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGA1100.32205176250146383No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1080.3161962759105282No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGATTGA1060.31034078931959247No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGA1050.3074130460241246No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACATCGATA1010.2957020728422532No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC970.2839910996603818No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG860.25178592341023537No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTGA830.24300269352383183No Hit
GCCACAACTGGGATTAGATACCCCTATAAGAGACAGTCTCAATCTAGAGG600.17566459772807122No Hit
GCCACAACTGGGATTAGATACCCCACAGAGGCAGATAGAGGAGCCTGTTC600.17566459772807122No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG530.15517039465979623No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG460.13467619159152125No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGTT108.43004E-4294.989788
CTGGATT400.0294.989788
TGGATTA400.0294.989789
TGGGTTA108.43004E-4294.989789
ACTGGGT108.43004E-4294.989787
CACTGGG255.2023097E-10294.989755
AACTGGG33250.0294.546146
CAACTGG33750.0294.11575
GCCACAA34000.0293.254521
ACTGGGA33350.0292.336187
CTGGGAT33400.0291.898568
TGGGATT33200.0291.879949
CCACAAC34550.0288.15932
CACAACT35000.0284.0333
ACAACTG35100.0282.803564
ACACTGG301.549779E-9245.824784
AACTGGA550.0241.355266
TGGGATA251.8560604E-7235.99189
ACTGGAT500.0235.99187
GAGCGCA150.0028379492196.65984295
Produced by FastQC (version 0.11.5)