FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-146-5-7-5-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-146-5-7-5-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34223
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG845424.702685328580195No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGA375010.9575431727201No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGCTG20576.010577681676066No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG18955.537211816614557No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGA13323.8921193349501797No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACGTGG13223.862899219822926No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC9032.638576395991No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG7822.2850130029512314No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTGGCT7312.135990415802238No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGACGTG5301.548666101744441No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT5101.4902258714899337No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGAC3861.127896443911989No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG3631.0606901791193057No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGA3521.0285480524793267No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG3481.0168600064284252No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGACGTGG3060.8941355228939601No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACGTG2680.7830990854103966No Hit
AAGCGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGAC2320.6779066709522835No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT2300.6720626479268329No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG2220.64868655582503No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG2080.6077783946468749No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT2030.5931683370832481No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGACGTGG1900.5551821874178184No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGAC1830.534728106828741No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG1650.4821318995996844No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAGAC1650.4821318995996844No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGACGTGG1610.47044385354878293No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG1470.42953569237062794No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG1410.41200362329427576No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGACG1360.397393565730649No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGACGTG1320.38570551967974753No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGACGTG1280.3740174736288461No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGACGT1270.3710954621161207No Hit
AAGCGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGACGT1200.3506413815270432No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG1190.34771937001431785No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT1170.3418753469888671No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGACGTGG1160.33895333547614176No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG1140.3331093124506911No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG1120.32726528942524036No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGCCGTAAACGATGGATA1110.324343277912515No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG1060.30973322034888817No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG1050.30681120883616286No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGACGTGG1040.30388919732343744No Hit
AAGCGACTGGGATTAGATACCCCCACGGGACCTAGAGGAGCCTGTTCTAG1020.2980451742979867No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG1010.29512316278526135No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGA990.2892791397598106No Hit
AAGCGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA980.28635712824708526No Hit
AAGCGACTGGGATTAGATACCCCGCACGGACCTAGAGGAGCCTGTTCTAG930.2717470706834585No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCCTG840.24544896706893024No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAG830.2425269555562049No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGACGTGG830.2425269555562049No Hit
AAGCGACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTAGACG820.23960494404347954No Hit
AAGCGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC780.22791689799257808No Hit
AAGCGACTGGGATTAGATACCCCTCCTAGAGGAGCCTGTTCTAGACGTGG730.2133068404289513No Hit
AAGCGACTGGGATTAGATACCCCTGCTGACTAGAGGAGCCTGTTCTAGAC730.2133068404289513No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCTAGAGGAGCCTGTTCTAGACG720.21038482891622595No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGTT710.2074628174035006No Hit
AAGCGACTGGGATTAGATACCCCTATAAGAGGAGCCTGTTCTAGACGTGG670.19577477135259916No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCTA660.19285275983987377No Hit
AAGCGACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAGA650.1899307483271484No Hit
AAGCGACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTAGACGTG650.1899307483271484No Hit
AAGCGACTGGGATTAGATACCCCGCCCTAGAGGAGCCTGTTCTAGACGTG650.1899307483271484No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACTAGAGGAGCCTGTTCTAGA630.1840867253016977No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGACGTGGC620.18116471378897234No Hit
AAGCGACTGGGATTAGATACCCCATGTGCTAGAGGAGCCTGTTCTAGACG590.17239867925079624No Hit
AAGCGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGAC570.16655465622534554No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGACGT540.15778862168716945No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGAC540.15778862168716945No Hit
AAGCGACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTAGA530.15486661017444409No Hit
AAGCGACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAGACG530.15486661017444409No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT520.15194459866171872No Hit
AAGCGACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAGAC480.1402565526108173No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGACGTGG480.1402565526108173No Hit
AAGCGACTGGGATTAGATACCCCATGTGTATAGAGGAGCCTGTTCTAGAC470.13733454109809193No Hit
AAGCGACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCCT470.13733454109809193No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG430.1256464950471905No Hit
AAGCGACTGGGATTAGATACCCCGAGTCTAGAGGAGCCTGTTCTAGACGT420.12272448353446512No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAGA420.12272448353446512No Hit
AAGCGACTGGGATTAGATACCCCGAGCCACGAGACTAGAGGAGCCTGTTC410.11980247202173977No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT400.1168804605090144No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG380.11103643748356369No Hit
AAGCGACTGGGATTAGATACCCCCGTCTAGAGGAGCCTGTTCTAGACGTG370.10811442597083833No Hit
AAGCGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAGACG360.10519241445811298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG157.1792238E-6295.09
GCGACTG33850.0295.03
CTGGGTT157.1792238E-6295.07
GACGGGA108.429218E-4295.05
ACGGGAT108.429218E-4295.06
GACTGGG33600.0295.05
TGGGTTA157.1792238E-6295.08
CGACGGG108.429218E-4295.04
ACTGGGT157.1792238E-6295.06
AGCGACG108.429218E-4295.02
CGACTGG33850.0294.564274
CTGGGAT33500.0294.55977
AAGCGAC34150.0294.136171
ACTGGGA33550.0294.120736
TGGGATT33500.0294.11948
AGCGACT34100.0293.26982
GGGATTA33650.0292.808329
CTGGATT251.8557876E-7236.07
GCGACGG150.002837672196.666673
CGGGATT150.002837672196.666677
Produced by FastQC (version 0.11.5)