FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-145-5-1-3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-145-5-1-3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34491
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC686919.91534023368415No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA368810.692644457974545No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCT23546.824968832449045No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT14644.244585544054971No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA13163.815488098344496No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACT13163.815488098344496No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT10142.9398973645298776No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCC9372.716650720477806No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCAC6942.012119103534255No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT6781.9657301904844742No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT5471.5859209648893913No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT5401.5656258154301124No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCACTC5081.47284798933055No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACC5061.4670493751993272No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCACT4171.20901104635992No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT3991.1568235191789162No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCAC3921.136528369719637No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT3811.1046359919979125No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAC3801.1017366849323011No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA3771.0930387637354673No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT3711.0756429213417993No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC3661.0611463860137427No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC3040.881389347945841No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAC2450.7103302310747731No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT2010.5827607201878751No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC1860.5392711142037053No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1830.5305731930068713No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT1460.4232988315792526No Hit
ATCGGTTACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT1430.41460091038241864No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1390.4030036821199733No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCAC1320.3827085326606941No Hit
ATCGGTTACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTACCAC1290.37401061146386017No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCACT1280.3711113043982488No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAC1230.35661476907019224No Hit
ATCGGTTACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTA1210.35081615493896956No Hit
ATCGGTTACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTACC1210.35081615493896956No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT1150.3334203125453017No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT1110.3218230842828564No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC1100.3189237772172451No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACCA1100.3189237772172451No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACCACT1020.2957293206923545No Hit
ATCGGTTACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCACT990.2870313994955206No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCTGCTAGAGGAGCCTGTTCTA900.2609376359050187No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACCA830.2406424864457395No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAC830.2406424864457395No Hit
ATCGGTTACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC810.23484387231451684No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT790.2290452581832942No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT780.22614595111768288No Hit
ATCGGTTACTGGGATTAGATACCCCATGTGTATAGAGGAGCCTGTTCTAC780.22614595111768288No Hit
ATCGGTTACTGGGATTAGATACCCCTTTTACATGTAAGGTCCTAGAGGAG770.22324664405207156No Hit
ATCGGTTACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTACCAC770.22324664405207156No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTG760.22034733698646025No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT740.2145487228552376No Hit
ATCGGTTACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTA720.20875010872401495No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT710.20585080165840364No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT690.200052187527181No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT640.1855556521991244No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAC620.17975703806790178No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT580.1681598098054565No Hit
ATCGGTTACTGGGATTAGATACCCCGGGCCCACGAGACCTAGAGGAGCCT560.16236119567423385No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGCACCTAGAGGA560.16236119567423385No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG530.1536632744773999No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAC530.1536632744773999No Hit
ATCGGTTACTGGGATTAGATACCCCATGCTAGAGGAGCCTGTTCTACCAC510.14786466034617726No Hit
ATCGGTTACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTACC510.14786466034617726No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTT480.1391667391493433No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT480.1391667391493433No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTACCA480.1391667391493433No Hit
ATCGGTTACTGGGATTAGATACCCCGAAACCTAGAGGAGCCTGTTCTACC470.136267432083732No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT450.13046881795250936No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCACT450.13046881795250936No Hit
ATCGGTTACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGT440.12756951088689802No Hit
ATCGGTTACTGGGATTAGATACCCCAGCCTAGAGGAGCCTGTTCTACCAC420.1217708967556754No Hit
ATCGGTTACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTACCA400.11597228262445276No Hit
ATCGGTTACTGGGATTAGATACCCCGACCCAACCTAGAGGAGCCTGTTCT400.11597228262445276No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA400.11597228262445276No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACCACTC380.11017366849323013No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG380.11017366849323013No Hit
ATCGGTTACTGGGATTAGATACCCCAATGTCTAGAGGAGCCTGTTCTACC380.11017366849323013No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCAC350.10147574729639616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTGG33950.0295.06
GTTACTG33950.0295.05
GTACTGG108.4294844E-4295.05
CGGTTCT157.1796803E-6294.999973
TCGGTTC157.1796803E-6294.999972
ATCGGTT34350.0294.57061
TCGGTTA34200.0294.568732
CGGTTAC34150.0294.568083
GGTTACT34050.0294.56684
TACTGGG33850.0294.12857
ACTGGGA33800.0293.690838
CTGGGAT33900.0292.82459
TACTGGA251.8559876E-7236.07
CTGGATT251.8559876E-7236.09
GGTTCTG202.2630258E-5221.254
TTCTGGG202.2630258E-5221.256
TCTGGGA202.2630258E-5221.257
CATTCTC150.002837762196.66666295
ATTATAG150.002837762196.66666295
TCGGTAC150.002837762196.666662
Produced by FastQC (version 0.11.5)