FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-144-6-7-4-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-144-6-7-4-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33338
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA1943258.28783970244166No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA13684.10342552042714No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA9282.7836102945587617No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA8452.534645149679045No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA6862.0577119203311534No Hit
TCGGTCAAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC6281.8837362769212311No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA5901.7697522346871437No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA4811.442798008278841No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA4781.4337992681024656No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA4391.3168156458095868No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA4041.2118303437518747No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA3751.1248425220469134No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA3511.052852600635911No Hit
TCGGTCAAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA3491.0468534405183274No Hit
TCGGTCAAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA2900.869878217049613No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGA2690.8068870358149859No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA2340.7019017337572739No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAA1980.5939168516407702No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA1750.5249265102885596No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATA1690.506929029935809No Hit
TCGGTCAAACTGGGATTAGATACCCCAGTAGTCTACGCCCTAAACGATGA1460.43793868858359825No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA1460.43793868858359825No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA1230.3689483472313876No Hit
TCGGTCAAACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACTATAA1220.36594876717259583No Hit
TCGGTCAAACTGGGATTAGATACCCCAATGTGGGCAAACGTCGATGCGTT1040.311956326114344No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA1020.3059571659967604No Hit
TCGGTCAAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1010.3029575859379687No Hit
TCGGTCAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC970.2909592657028016No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAGCCA910.272961785350051No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAA890.26696262523246744No Hit
TCGGTCAAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC820.24596556482092508No Hit
TCGGTCAAACTGGGATTAGATACCCCAAGACAAGAAATTGATTTAAAATA810.24296598476213332No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGA710.2129701841742156No Hit
TCGGTCAAACTGGGATTAGATACCCCACAGCCCCGGCTGCAAGAGGTCGC600.17997480352750614No Hit
TCGGTCAAACTGGGATTAGATACCCCCTTCGCCCATTACGGATCTTCACG540.16197732317475552No Hit
TCGGTCAAACTGGGATTAGATACCCCGGTAGTCCACGCCCTAAACGCTGT520.155978163057172No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAA510.1529785829983802No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA500.14997900293958846No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTCAACCCTAAACTCGAA490.14697942288079668No Hit
TCGGTCAAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGA490.14697942288079668No Hit
TCGGTCAAACTGGGATTAGATACCCCAGTGATTGTTGGTGGTTGCGCCAC430.12898194252804607No Hit
TCGGTCAAACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAAC410.12298278241046254No Hit
TCGGTCAAACTGGGATTAGATACCCCATGCCCTGCGCATCCAGTTGGCGA370.11098446217529545No Hit
TCGGTCAAACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC360.10798488211650369No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGA108.429121E-4294.98959
ATGGGAT108.429121E-4294.98959
AAACCGG108.429121E-4294.98957
AACCGGG108.429121E-4294.98958
TCGGTCG108.429121E-4294.98951
AGAGGCC108.429121E-4294.9895295
AACTAGG108.429121E-4294.98958
ACGGGAT108.429121E-4294.98959
CGGTCGA108.429121E-4294.98952
AACGGGA108.429121E-4294.98958
AAACGGG108.429121E-4294.98957
CAAACGG108.429121E-4294.98956
CAAATGG108.429121E-4294.98956
CCTAGGA108.429121E-4294.9895295
AAATGGG108.429121E-4294.98957
TCGGTCA33050.0294.989471
GGTCAAA32850.0294.989473
CGGTCAA33050.0294.989472
AAACTGG32400.0294.534277
GTCAAAC32850.0293.64254
Produced by FastQC (version 0.11.5)