FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-143-4-8-2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-143-4-8-2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34998
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA463613.246471226927254No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG440312.580718898222756No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAAC28298.083319046802675No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTGAG17164.903137322132693No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG14874.248814217955312No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA10593.0258871935539173No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGAA9052.585862049259958No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAG8662.4744271101205784No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTGAG7812.231556088919367No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTGA6721.9201097205554603No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA5911.6886679238813647No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC5671.6200925767186698No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGAGA5621.605806046059775No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA4761.3600777187267843No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA4521.2915023715640894No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGCT4361.245785473455626No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAG3370.962912166409509No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA3350.9571975541459512No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2820.8057603291616663No Hit
CAACACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGCTG2720.7771872678438768No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCTGA2670.762900737184982No Hit
CAACACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGC2210.6314646551231499No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA2080.5943196754100234No Hit
CAACACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGCT2010.5743185324875708No Hit
CAACACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGCTGAG1990.5686039202240127No Hit
CAACACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT1870.5343162466426653No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1860.5314589405108864No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC1740.4971712669295389No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT1670.47717012400708614No Hit
CAACACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGCTGAG1670.47717012400708614No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCTGA1630.46574089947997027No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA1600.4571689810846334No Hit
CAACACACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT1530.43716783816218063No Hit
CAACACACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTAGCTG1500.4285959197668438No Hit
CAACACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGC1480.4228813075032859No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA1470.4200240013715069No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1380.39430824618549626No Hit
CAACACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGC1370.3914509400537174No Hit
CAACACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAG1190.3400194296816961No Hit
CAACACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGCTGAG1170.33430481741813817No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAGC1150.32859020515458026No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA1080.30858906223212756No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1020.2914452254414538No Hit
CAACACACTGGGATTAGATACCCCAACGAACCTAGAGGAGCCTGTTCTAG990.28287330704611696No Hit
CAACACACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAGCT990.28287330704611696No Hit
CAACACACTGGGATTAGATACCCCATAATCCTAGAGGAGCCTGTTCTAGC960.2743013886507801No Hit
CAACACACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCC950.2714440825190011No Hit
CAACACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAG930.2657294702554432No Hit
CAACACACTGGGATTAGATACCCCCCTAACACTAGAGGAGCCTGTTCTAG920.2628721641236642No Hit
CAACACACTGGGATTAGATACCCCAATGTCTAGAGGAGCCTGTTCTAGCT910.2600148579918853No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA900.2571575518601063No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGC850.2428710212012115No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC850.2428710212012115No Hit
CAACACACTGGGATTAGATACCCCAGTGTCAATCACTGTCTCTAGAGGAG820.23429910280587463No Hit
CAACACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGCTG800.2285844905423167No Hit
CAACACACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCTAG770.22001257214697983No Hit
CAACACACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCT760.21715526601520088No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA760.21715526601520088No Hit
CAACACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGCTGA720.20572604148808502No Hit
CAACACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGCTGA710.20286873535630606No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA690.19715412309274813No Hit
CAACACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGCTGAG680.1942968169609692No Hit
CAACACACTGGGATTAGATACCCCTCCGACCCACGAGACCTAGAGGAGCC640.18286759243385337No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGC630.1800102863020744No Hit
CAACACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGCTGAG620.17715298017029543No Hit
CAACACACTGGGATTAGATACCCCATGCTAGAGGAGCCTGTTCTAGCTGA620.17715298017029543No Hit
CAACACACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAG600.17143836790673753No Hit
CAACACACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAGCT540.15429453111606378No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA520.14857991885250585No Hit
CAACACACTGGGATTAGATACCCCAGGAGGAGCCTGTTCTAGCTGAGAAC520.14857991885250585No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAGC500.14286530658894794No Hit
CAACACACTGGGATTAGATACCCCACATGATGGTGATTTTATAACAGCAC490.14000800045716896No Hit
CAACACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGCTGAG490.14000800045716896No Hit
CAACACACTGGGATTAGATACCCCCGTCTAGAGGAGCCTGTTCTAGCTGA490.14000800045716896No Hit
CAACACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGCTG480.13715069432539004No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT480.13715069432539004No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA480.13715069432539004No Hit
CAACACACTGGGATTAGATACCCCGAGATGCCTAAGCTAGAGGAGCCTGT470.13429338819361106No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA450.12857877593005315No Hit
CAACACACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAG450.12857877593005315No Hit
CAACACACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGCTG420.12000685753471627No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA410.11714955140293731No Hit
CAACACACTGGGATTAGATACCCCCACCCAACCTAGAGGAGCCTGTTCTA410.11714955140293731No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGCTGAG390.11143493913937938No Hit
CAACACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGC390.11143493913937938No Hit
CAACACACTGGGATTAGATACCCCCGCTAGAGGAGCCTGTTCTAGCTGAG390.11143493913937938No Hit
CAACACACTGGGATTAGATACCCCGCACCTAGAGGAGCCTGTTCTAGCTG370.10572032687582147No Hit
CAACACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAG360.10286302074404251No Hit
CAACACACTGGGATTAGATACCCCACCCCAGGACATAAAGCTAGAGGAGC360.10286302074404251No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA206.103073E-8294.969977
CACTGGG33900.0294.969976
CGCTGGG206.103073E-8294.969976
ACGGGAT108.432581E-4294.969977
CGGGATT108.432581E-4294.969978
ACCCTGG157.183493E-6294.969945
TGGATTA255.2023097E-10294.969949
CACCCTG157.183493E-6294.969944
CTGGGAT34250.0294.539348
ACTGGGA33950.0294.535557
TGGGATT34250.0294.108739
ACACTGG34350.0293.68195
CACACTG35100.0288.2474
ACACACT37300.0271.641243
CACTGGA351.2732926E-11252.831396
CTGGATT301.549779E-9245.808298
CAACACA42700.0238.670071
ACGCTGG251.8573155E-7235.975975
AACACAC44800.0226.8242
ACTGGAT403.274181E-11221.227467
Produced by FastQC (version 0.11.5)