FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-142-5-7-5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-142-5-7-5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33299
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG840425.23799513498904No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACC33299.997297216132617No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCTGT21726.52271839995195No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG16895.072224391122856No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACTCC10513.1562509384666204No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTACC10463.141235472536713No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC8982.696777681011442No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCACTC7112.13519925523289No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG6902.0721342983272772No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTACC5461.6396888795459321No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT5311.5946424817562088No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCACTCC5261.579627015826301No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT4901.4715156611309648No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCACTC4331.300339349530016No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG3761.129163037929067No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCACTCCT3451.0360671491636386No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCCTG3130.9399681672122286No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAC2820.8468722784468002No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG2610.7838073215411875No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAC2520.7567794828673533No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACCACT2510.7537763896813718No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACCA2290.6877083395897775No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT2270.6817021532178144No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG2130.6396588486140725No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAC1580.47448872338508663No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG1570.4714856301991051No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC1430.4294423255953632No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG1420.42643923240938164No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG1390.41742995285143697No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCACTC1290.3873990209916214No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACCA1270.3813928346196582No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCACTCC1250.37538664824769513No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG1210.3633742755037689No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT1180.3543649959458242No Hit
AAGCGACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCCT1120.33634643682993487No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACCA1120.33634643682993487No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTACC1060.31832787771404547No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTAC1050.3153247845280639No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG1030.3093185981561008No Hit
AAGCGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTACC1020.3063155049701192No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACCACTCC1010.30331241178413765No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG940.28229075948226673No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCACTC930.2792876662962852No Hit
AAGCGACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTACCAC910.27328147992432206No Hit
AAGCGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG890.2672752935523589No Hit
AAGCGACTGGGATTAGATACCCCTCTTATCACATCTGACGCTAGAGGAGC830.2492567344364696No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG790.23724436169254332No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAC760.22823508213459862No Hit
AAGCGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTACCACTCC750.2252319889486171No Hit
AAGCGACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTACCAC740.22222889576263552No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG720.2162227093906724No Hit
AAGCGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTACCA710.21321961620469082No Hit
AAGCGACTGGGATTAGATACCCCTGACGCGACGACTAGAGGAGCCTGTTC690.20721342983272772No Hit
AAGCGACTGGGATTAGATACCCCATGCTAGAGGAGCCTGTTCTACCACTC670.2012072434607646No Hit
AAGCGACTGGGATTAGATACCCCCGTACGTGCAGGGAGGGTACCTTGAGG650.19520105708880148No Hit
AAGCGACTGGGATTAGATACCCCGACGACTAGAGGAGCCTGTTCTACCAC650.19520105708880148No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT640.1921979639028199No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACCACTCC640.1921979639028199No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACCAC620.18619177753085678No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAC600.18018559115889368No Hit
AAGCGACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTCTA580.17417940478693053No Hit
AAGCGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACCACT580.17417940478693053No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACCACT570.17117631160094898No Hit
AAGCGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG550.16517012522898586No Hit
AAGCGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTACCA540.16216703204300428No Hit
AAGCGACTGGGATTAGATACCCCCGCCACCTAGAGGAGCCTGTTCTACCA540.16216703204300428No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCACTCC530.15916393885702274No Hit
AAGCGACTGGGATTAGATACCCCGTGCTAGAGGAGCCTGTTCTACCACTC530.15916393885702274No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCACTCC510.1531577524850596No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTACCA510.1531577524850596No Hit
AAGCGACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTACCACTC510.1531577524850596No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCCTG500.15015465929907804No Hit
AAGCGACTGGGATTAGATACCCCTGTGTATAAGAGGAGCCTGTTCTACCA470.14114537974113336No Hit
AAGCGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTACCACT470.14114537974113336No Hit
AAGCGACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA460.13814228655515182No Hit
AAGCGACTGGGATTAGATACCCCCCTCCGACAATCGCGATTCGAACTGGA410.12312682062524401No Hit
AAGCGACTGGGATTAGATACCCCGAGATCTAGAGGAGCCTGTTCTACCAC400.12012372743926245No Hit
AAGCGACTGGGATTAGATACCCCACGAGCTAGAGGAGCCTGTTCTACCAC400.12012372743926245No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGATCTAGAGGAGCC390.11712063425328087No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT390.11712063425328087No Hit
AAGCGACTGGGATTAGATACCCCGACTTAGAGGAGCCTGTTCTACCACTC390.11712063425328087No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT370.11111444788131776No Hit
AAGCGACTGGGATTAGATACCCCAAGCCCGACTAGAGGAGCCTGTTCTAC340.10210516832337307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT157.179473E-6294.978976
GATGGGA157.179473E-6294.978975
GCGATGG157.179473E-6294.978973
CGATGGG157.179473E-6294.978974
ACTGGGA32650.0294.978976
AGCGACG108.429966E-4294.978942
GACTGGG32700.0294.978945
GACTGGA206.097798E-8294.978945
GCGACTG32950.0294.97893
AAGCGAC33150.0294.08911
CGACTGG33000.0294.085084
CTGGGAT32750.0294.078257
TGGGATT32750.0293.62798
AGCGACT33150.0293.199282
GGGATTA32750.0293.177559
GCGACGG150.0028379238196.652653
TGGATTA304.6045716E-7196.652658
TGGGATA150.0028379238196.652658
CTGGATT304.6045716E-7196.652657
GGGATAG150.0028379238196.652659
Produced by FastQC (version 0.11.5)