FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-141-5-7-5-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-141-5-7-5-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33121
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG840825.385706953292477No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGC32469.800428731016575No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG18995.733522538570695No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAACT18445.5674647504604335No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTGAGA11563.4902327828266055No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC8802.656924609764198No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG6982.1074242927447844No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGC6331.911174179523565No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGAGAA6291.8990972494791825No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGAAC5661.7088856012801548No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG5591.687750973702485No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT5201.5700009057697533No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTGAG4831.4582893028592132No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGC4831.4582893028592132No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT4751.4341354427704478No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT4101.2378853295492285No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG3551.0718275414389662No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCTGAG3000.9057697533287038No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAG2930.8846351257510342No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG2780.8393466380845989No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGC2660.8031158479514509No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTGAGA2400.7246158026629631No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG2110.6370580598411885No Hit
AAGCGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA1990.6008272697080402No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGCTGAGA1850.5585580145527007No Hit
AAGCGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG1660.5011925968418828No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGCT1620.48911566679750007No Hit
AAGCGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAGCT1610.4860964342864044No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG1570.47401950424202166No Hit
AAGCGACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGCTGA1550.4679810392198303No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGCTGAG1500.4528848766643519No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG1480.4468464116421606No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGCT1460.44080794661996925No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG1410.42571178406449084No Hit
AAGCGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAGC1370.4136348540201081No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG1340.404577156486821No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG1150.34721173877600314No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGCT1100.33211557622052473No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGCTG1080.3260771111983334No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAGCT1060.320038646176142No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG1060.320038646176142No Hit
AAGCGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGCTGAGAA980.2958847860873766No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGCTGAGA950.28682708855408956No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG930.2807886235318982No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG900.27173092599861115No Hit
AAGCGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGCT890.26871169348751545No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCTGAG870.2626732284653241No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG860.25965399595422844No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGCTGA850.25663476344313274No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC840.2536155309320371No Hit
AAGCGACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTAGCTGA830.2505962984209414No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT770.23248090335436733No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGCT770.23248090335436733No Hit
AAGCGACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTAGC690.2083270432656019No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGCTGA680.20530781075450621No Hit
AAGCGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAGCTG600.18115395066574078No Hit
AAGCGACTGGGATTAGATACCCCGGCAGACCTAGAGGAGCCTGTTCTAGC600.18115395066574078No Hit
AAGCGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGCTGA600.18115395066574078No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG580.17511548564354942No Hit
AAGCGACTGGGATTAGATACCCCCACCCTAGAGGAGCCTGTTCTAGCTGA580.17511548564354942No Hit
AAGCGACTGGGATTAGATACCCCCCATCTAGAGGAGCCTGTTCTAGCTGA530.160019323088071No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGCTGA530.160019323088071No Hit
AAGCGACTGGGATTAGATACCCCAAACTAGAGGAGCCTGTTCTAGCTGAG530.160019323088071No Hit
AAGCGACTGGGATTAGATACCCCCCAACCTAGAGGAGCCTGTTCTAGCTG530.160019323088071No Hit
AAGCGACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA500.15096162555478396No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAGCT470.14190392802149693No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAGCT470.14190392802149693No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT440.1328462304882099No Hit
AAGCGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAGC440.1328462304882099No Hit
AAGCGACTGGGATTAGATACCCCTGACGCTGCTAGAGGAGCCTGTTCTAG430.12982699797711422No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGTT420.12680776546601855No Hit
AAGCGACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGCTG410.12378853295492286No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT400.12076930044382717No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG400.12076930044382717No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAGC400.12076930044382717No Hit
AAGCGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGCTGA390.1177500679327315No Hit
AAGCGACTGGGATTAGATACCCCGAAACCTAGAGGAGCCTGTTCTAGCTG380.11473083542163581No Hit
AAGCGACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGAGC380.11473083542163581No Hit
AAGCGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTGT370.11171160291054015No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACAG360.10869237039944446No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATGA360.10869237039944446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG206.0992534E-8294.960724
CTGGGTT206.0992534E-8294.960727
GCGCTGG206.0992534E-8294.960723
TGGGTTA206.0992534E-8294.960728
ACTGGGT206.0992534E-8294.960726
GCGACTG32850.0294.511783
GACTGGG32650.0294.509035
ACTGGGA32450.0294.506236
CGACTGG32900.0294.06424
CTGGGAT32600.0293.151157
AAGCGAC33100.0292.73291
AGCGACT33100.0292.73292
TGGGATT32450.0292.68838
GGGATTA32450.0292.23389
GGGTTAG251.856115E-7235.96869
GCTGGGA251.856115E-7235.96865
CTGGATT251.856115E-7235.96867
AAGCGCT251.856115E-7235.96861
AGCGCTG251.856115E-7235.96862
TGGGATA251.856115E-7235.96868
Produced by FastQC (version 0.11.5)