FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-140-6-7-4-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-140-6-7-4-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32216
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA1902359.048298981872364No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA11523.575862925254532No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA9713.0140302955053393No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA8802.7315619567916563No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA8642.6818971939408986No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA6522.0238390861683637No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA5331.6544574124658553No Hit
TCGGTCAAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA5131.5923764589024088No Hit
TCGGTCAAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA4131.281971691085175No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA3551.1019369257511795No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGA2980.9250062080953563No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA2540.7884281102557735No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA2360.7325552520486714No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA2190.6797864415197417No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAA2130.6611621554507077No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA1870.580456915818227No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA1620.5028557238639185No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAA1610.49975167618574623No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATAGA1560.48423143779488453No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAGACCTCAA1410.4376707226222995No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA1390.4314626272659548No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCATAAACATTGG1350.4190464365532655No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAA1310.4066302458405761No Hit
TCGGTCAAACTGGGATTAGATACCCCCACTATGCTTAGCCCTAAACCTCA1290.4004221504842314No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCTCTAAACCTCAA1190.3693816737025081No Hit
TCGGTCAAACTGGGATTAGATACCCCAGTAGTCTACGCCCTAAACGATGA1080.33523714924261233No Hit
TCGGTCAAACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAA1080.33523714924261233No Hit
TCGGTCAAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC1020.31661286317357834No Hit
TCGGTCAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC980.304196672460889No Hit
TCGGTCAAACTGGGATTAGATACCCCACTACGCTTAGCCCTAAAGTCGAA940.29178048174819965No Hit
TCGGTCAAACTGGGATTAGATACCCCAATGTGGGCAAACGTCGATGCGTT920.28557238639185495No Hit
TCGGTCAAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC830.25763595728830396No Hit
TCGGTCAAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTATAAG640.19865905140302956No Hit
TCGGTCAAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAG630.1955550037248572No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTATAA580.18003476533399554No Hit
TCGGTAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC570.1769307176558232No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAA550.17072262229947852No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA540.16761857462130617No Hit
TCGGTCAAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC530.16451452694313384No Hit
TCGGTCAAACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACTATAA440.1365780978395828No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAG440.1365780978395828No Hit
TCGGTCAAACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAAC420.13037000248323816No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA400.12416190712689348No Hit
TCGGTCAAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTA390.12105785944872113No Hit
TCGGTCAAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTC380.11795381177054878No Hit
TCGGTCAAACTGGGATTAGATACCCCGGCACCTGTTAATACAGCTACTCT360.11174571641420412No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA350.1086416687360318No Hit
TCGGTCAAACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC350.1086416687360318No Hit
TCGGTCAAATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC340.10553762105785945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTCA32100.0295.01
ACGGGAT108.4269274E-4295.09
CGGTCAA32050.0295.02
AACGGGA108.4269274E-4295.08
AAACGGG108.4269274E-4295.07
CAAACGG108.4269274E-4295.06
TGACATA157.175322E-6294.99997295
GGTCAAA31950.0294.538333
AAACTGG31850.0294.07387
GTCAAAC32000.0293.61724
AACTGGG31900.0293.150488
ACTGGGA31900.0293.150489
TCAAACT32100.0291.783485
CAAACTG32200.0290.877326
CAACTGG202.2616547E-5221.256
GTCAACT202.2616547E-5221.254
TCAACTG202.2616547E-5221.255
GGTCAAC202.2616547E-5221.253
TCAAACG150.0028369033196.666665
GCCCACA359.916093E-7168.57143295
Produced by FastQC (version 0.11.5)