FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-14-4-8-2-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-14-4-8-2-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11975
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA225018.789144050104383No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA135711.331941544885177No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAAGTCTCCTG8176.822546972860125No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAAGTCTC6385.32776617954071No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA4453.7160751565762005No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAA3562.9728601252609606No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC2942.4551148225469728No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA2241.8705636743215033No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAAGTCTCCT2181.8204592901878913No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAAGTCT1891.5782881002087683No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAAGTCTC1821.5198329853862214No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA1721.4363256784968685No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT1701.4196242171189979No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1611.3444676409185805No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAA1601.336116910229645No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC1451.210855949895616No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAAGTCT1381.152400835073069No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAA1321.102296450939457No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1281.0688935281837162No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA1261.0521920668058455No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAAGTCTCC1100.918580375782881No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA930.7766179540709812No Hit
CAACACACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTAAGTC760.6346555323590815No Hit
CAACACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAAGTCT630.5260960334029228No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAAGTCT630.5260960334029228No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAAG610.5093945720250522No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA580.4843423799582463No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAAG570.47599164926931103No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA570.47599164926931103No Hit
CAACACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAAGTCTC560.4676409185803758No Hit
CAACACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAA540.4509394572025052No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA520.4342379958246346No Hit
CAACACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAAG470.3924843423799582No Hit
CAACACACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT460.38413361169102295No Hit
CAACACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAAG440.3674321503131524No Hit
CAACACACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTAA440.3674321503131524No Hit
CAACACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA430.35908141962421714No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAGT420.3507306889352818No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC410.34237995824634654No Hit
CAACACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAAGT400.33402922755741127No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAAGTCTCC400.33402922755741127No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA390.325678496868476No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA390.325678496868476No Hit
CAACACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAAG390.325678496868476No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA380.3173277661795407No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT370.3089770354906054No Hit
CAACACACTGGGATTAGATACCCCTCTTATACACATCTACGCTAGAGGAG370.3089770354906054No Hit
CAACACACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTTC360.3006263048016702No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAAG360.3006263048016702No Hit
CAACACACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGG300.25052192066805845No Hit
CAACACACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTAAGTCT290.24217118997912315No Hit
CAACACACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAA270.2254697286012526No Hit
CAACACACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAAG260.2171189979123173No Hit
CAACACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAAGTC250.20876826722338201No Hit
CAACACACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAAGTC240.20041753653444677No Hit
CAACACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTAAGTCTCC240.20041753653444677No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA230.19206680584551147No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA220.1837160751565762No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG220.1837160751565762No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA220.1837160751565762No Hit
CAACACACTGGGATTAGATACCCTATAAGAGGAGCCTGTTCTAAGTCTCC210.1753653444676409No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT210.1753653444676409No Hit
CAACACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAAGTCTC210.1753653444676409No Hit
CAACACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAAGTC210.1753653444676409No Hit
CAACACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAAGTCTC210.1753653444676409No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA200.16701461377870563No Hit
CAACACACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTAAGTCT200.16701461377870563No Hit
CAACACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT190.15866388308977036No Hit
CAACACACTGGGATTAGATACCCCTAGCCCACGAGACCTAGAGGAGCCTG190.15866388308977036No Hit
CAACACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA180.1503131524008351No Hit
CAACACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAAGTCTC180.1503131524008351No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG160.13361169102296452No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATAT160.13361169102296452No Hit
CAACACACTGGGATTAGATACCCCAATGTCTAGAGGAGCCTGTTCTAAGT150.12526096033402923No Hit
CAACACACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTAAGTCT150.12526096033402923No Hit
CAACACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAAGTC140.11691022964509395No Hit
CAACACACTGGGATTAGATACCCATAGAGGAGCCTGTTCTAAGTCTCCTG130.10855949895615866No Hit
CAACACGCTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA130.10855949895615866No Hit
CAACACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAAGTC130.10855949895615866No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT120.10020876826722339No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA120.10020876826722339No Hit
CAACACACTGGGATTAGATACCCCACGAACTAGAGGAGCCTGTTCTAAGT120.10020876826722339No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATG120.10020876826722339No Hit
CAACACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAAGTCT120.10020876826722339No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT11700.0294.941448
CACTGGG11550.0294.941446
ACTGGGA11550.0294.941447
ACACGGG108.3656603E-4294.94145
CACACGG108.3656603E-4294.94144
CACCCTG108.3656603E-4294.94144
CTGGATT108.3656603E-4294.94148
CCCTGGG108.3656603E-4294.94146
ACCCTGG108.3656603E-4294.94145
TGGGATT11750.0294.94149
CCTGGGA108.3656603E-4294.94147
ACACTGG11700.0293.6815
CACACTG11850.0289.96354
ACACACT12550.0273.790253
CAACACA15050.0231.249741
AACACAC15100.0229.50742
CACTGGA150.0028163327196.627616
TGGATTA150.0028163327196.627619
ACTGGAT150.0028163327196.627617
GCCCTAA3000.059.23613735-39
Produced by FastQC (version 0.11.5)