FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-139-4-1-3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-139-4-1-3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33246
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA462813.920471635685496No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCT336510.121518378150755No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC29408.843169103049991No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACT17775.3450039102448414No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA17555.278830536004332No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT11673.5101967153943328No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCC10973.2996450700836193No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCAC8652.6018167599109665No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCACTC7132.1446189015219876No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT6662.0032485110990796No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACC6331.9039884497383146No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCAC5861.7626180593154062No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT5331.6032003850087229No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCACT5171.5550742946519882No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA5051.5189797268844372No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT4921.4798772784695904No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC4921.4798772784695904No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAC4821.4497984719966313No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT3421.028695181375203No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAC3190.9595139264873969No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC2550.7670095650604584No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT2400.7218913553510197No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT2180.6557179811105095No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT2120.6376706972267341No Hit
ATCGGTTACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTA2090.6286470552848463No Hit
ATCGGTTACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT1960.5895446068699994No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1960.5895446068699994No Hit
ATCGGTTACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTACCAC1870.5624736810443361No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCACT1550.46622150033086684No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAC1550.46622150033086684No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACCACT1430.4301269325633159No Hit
ATCGGTTACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTACC1420.42711905191601995No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACCA1350.4060638873849486No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1240.3729772002646935No Hit
ATCGGTTACTGGGATTAGATACCCCATGTGTATAGAGGAGCCTGTTCTAC1220.36696143897010164No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCAC1200.36094567767550984No Hit
ATCGGTTACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCACT1180.354929916380918No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT1150.34590627443903027No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT1140.34289839379173437No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCTGCTAGAGGAGCCTGTTCTA1080.32485110990795885No Hit
ATCGGTTACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC1060.31883534861336704No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT1050.3158274679660711No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAC1040.3128195873187752No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACCA1020.3068038260241834No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAC990.2977801840822956No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT940.28274078084581605No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTACCA860.25867773566744867No Hit
ATCGGTTACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTACCAC800.24063045178367323No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTT770.23160680984178547No Hit
ATCGGTTACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGT760.22859892919448957No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAC740.22258316789989774No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC740.22258316789989774No Hit
ATCGGTTACTGGGATTAGATACCCCTTTTACATGTAAGGTCCTAGAGGAG720.2165674066053059No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCACT720.2165674066053059No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT700.21055164531071408No Hit
ATCGGTTACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTA700.21055164531071408No Hit
ATCGGTTACTGGGATTAGATACCCCGACCCAACCTAGAGGAGCCTGTTCT680.20453588401612224No Hit
ATCGGTTACTGGGATTAGATACCCCGAAACCTAGAGGAGCCTGTTCTACC630.18949648077964265No Hit
ATCGGTTACTGGGATTAGATACCCCAATGTCTAGAGGAGCCTGTTCTACC620.18648860013234675No Hit
ATCGGTTACTGGGATTAGATACCCCATGCTAGAGGAGCCTGTTCTACCAC610.18348071948505082No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT600.18047283883775492No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCAC580.17445707754316309No Hit
ATCGGTTACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTACC550.16543343560127535No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT540.16242555495397942No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT530.15941767430668352No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACCACTC480.14437827107020393No Hit
ATCGGTTACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTACCA480.14437827107020393No Hit
ATCGGTTACTGGGATTAGATACCCCACGTCTAGAGGAGCCTGTTCTACCA470.14137039042290803No Hit
ATCGGTTACTGGGATTAGATACCCCGGGCCCACGAGACCTAGAGGAGCCT440.13234674848102027No Hit
ATCGGTTACTGGGATTAGATACCCCTGTATAAGAGGAGCCTGTTCTACCA440.13234674848102027No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTACC430.12933886783372434No Hit
ATCGGTTACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACCA410.12332310653913252No Hit
ATCGGTTACTGGGATTAGATACCCCAGCCTAGAGGAGCCTGTTCTACCAC400.12031522589183662No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC380.11429946459724478No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGCACCTAGAGGA380.11429946459724478No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT360.10828370330265295No Hit
ATCGGTTACTGGGATTAGATACCCCAGATGTGTATAAGAGGAGCCTGTTC360.10828370330265295No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT350.10527582265535704No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCAGAACCTAGAGGAGCC350.10527582265535704No Hit
ATCGGTTACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTACC340.10226794200806112No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGGG108.429911E-4294.97895
CGGTTTG108.429911E-4294.97893
GGTTTGG108.429911E-4294.97894
TTTGGGA108.429911E-4294.97896
ATCGGTG108.429911E-4294.97891
TCGGTTA32650.0294.97892
TTACTGG32600.0294.978886
ATCGGTA255.2023097E-10294.978881
GGTTACT32600.0294.978884
ATCGGTT32900.0294.530641
CGGTTAC32700.0294.52793
GTTACTG32700.0294.076845
TACTGGG32750.0292.727177
ACTGGGA32800.0292.280948
CTGGGAT32900.0291.392559
GGTACTG301.54796E-9245.815774
CGGTACT301.54796E-9245.815773
GTACTGG353.8926373E-9210.699235
TCGGTAC353.8926373E-9210.699232
TCGGTTT150.0028379066196.65262
Produced by FastQC (version 0.11.5)