FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-138-5-2-6-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-138-5-2-6-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31530
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA922329.251506501744373No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA20736.574690770694576No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG20736.574690770694576No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTGA17345.499524262607041No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGAC16605.264827148747225No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA9493.0098319061211543No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA8092.56581033935934No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG7112.2549952426260704No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT4801.5223596574690772No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAGA4781.5160164922296226No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA4401.3954963526799873No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATTG4131.3098636219473518No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA3741.186171899777989No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGATTGAG3641.1544560735807168No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA3241.027592768791627No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA3231.0244211861718997No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGATTGA2940.9324452901998096No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG2790.884871550903901No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGATTG2390.7580082461148113No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA2370.7516650808753568No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAG2210.7009197589597209No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA1880.5962575325087218No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGATTG1860.5899143672692674No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA1850.5867427846495401No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA1840.5835712020298128No Hit
GCCACAACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGATT1460.46305106248017763No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA1440.45670789724072314No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA1430.45353631462099586No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA1290.4091341579448145No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA1280.4059625753250872No Hit
GCCACAACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGATT1160.3679035838883603No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAG1140.36156041864890576No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT1100.3488740881699968No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1050.3330161750713606No Hit
GCCACAACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA1010.3203298445924516No Hit
GCCACAACTGGGATTAGATACCCCGAGCACTAGAGGAGCCTGTTCTAGAT1000.3171582619727244No Hit
GCCACAACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTAG950.3013003488740882No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT860.272756105296543No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA810.25689819219790677No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC810.25689819219790677No Hit
GCCACAACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAG770.2442118617189978No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA750.23786869647954328No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC740.23469711385981606No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA700.22201078338090707No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGATTGA690.21883920076117983No Hit
GCCACAACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGATT670.21249603552172536No Hit
GCCACAACTGGGATTAGATACCCCTAGTAGTCCTAGAGGAGCCTGTTCTA670.21249603552172536No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGA650.20615287028227083No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT640.2029812876625436No Hit
GCCACAACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAG640.2029812876625436No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA600.19029495718363465No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGA600.19029495718363465No Hit
GCCACAACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT580.18395179194418015No Hit
GCCACAACTGGGATTAGATACCCCAACCGTCGTTCCACAATCGGCTCAAA560.17760862670472566No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGATTGA560.17760862670472566No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGATTGAG550.1744370440849984No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGATTG520.1649222962258167No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG490.15540754836663495No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGA470.14906438312718046No Hit
GCCACAACTGGGATTAGATACCCCACGAGGCCTAGAGGAGCCTGTTCTAG460.1458928005074532No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG420.13320647002854424No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGA410.130034887408817No Hit
GCCACAACTGGGATTAGATACCCCCCGAGACCTAGAGGAGCCTGTTCTAG410.130034887408817No Hit
GCCACAACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGATTG410.130034887408817No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA400.12686330478908975No Hit
GCCACAACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAGA390.12369172216936251No Hit
GCCACAACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGATTG390.12369172216936251No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA380.12052013954963527No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGGGACTAGAGGAGCCTGT340.1078338090707263No Hit
GCCACAACTGGGATTAGATACCCCAAGCCCACGAGACTAGAGGAGCCTGT340.1078338090707263No Hit
GCCACAACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGG330.10466222645099904No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC330.10466222645099904No Hit
GCCACAACTGGGATTAGATACCCCTGTAGCCCCTAGAGGAGCCTGTTCTA330.10466222645099904No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTGG108.427007E-4294.98896
CGGGATT157.1749164E-6294.98898
ACGGGAT157.1749164E-6294.98897
CAACGGG157.1749164E-6294.98895
CAACTGG31100.0294.514625
AACTGGG30950.0294.512336
ACTGGGA30900.0294.511547
CTGGGAT30900.0294.03428
TGGGATT30850.0293.554579
GCCACAA31600.0293.121861
CCACAAC32200.0287.20192
CACAACT32300.0284.94283
ACAACTG32400.0283.152924
CTGGATT202.2615277E-5221.241678
CAACTTG150.0028369306196.659265
TGGGATA150.0028369306196.659269
AACTGGA255.5064575E-5176.993336
TGGATTA255.5064575E-5176.993339
AACGGGA255.5064575E-5176.993336
AACCTGA200.006707591147.49445295
Produced by FastQC (version 0.11.5)