FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-137-6-7-4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-137-6-7-4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30144
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA1849661.3588110403397No Hit
TCGGTCAAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA10893.61265923566879No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA10573.506502123142251No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA9103.0188428874734607No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA7372.444930997876858No Hit
TCGGTCAAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC5851.940684713375796No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA5191.7217356687898089No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA4321.4331210191082804No Hit
TCGGTCAAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA3331.1046974522292994No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA2960.9819532908704883No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA2900.9620488322717622No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA2820.9355095541401274No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAA2660.8824309978768577No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA2590.8592091295116773No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA2560.8492569002123143No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA2190.7265127388535032No Hit
TCGGTCAAACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACTATAA1640.5440552016985137No Hit
TCGGTCAAACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAAT1550.5141985138004246No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA1460.48434182590233543No Hit
TCGGTCAAACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACCCTGAT1230.40804140127388533No Hit
TCGGTCAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1060.35164543524416136No Hit
TCGGTCAAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC990.3284235668789809No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATTCTTAGCCCTAAACCTCAA690.2289012738853503No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAA670.2222664543524416No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATTCCTGGCCCTAAATCTTGA630.2089968152866242No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAA580.19240976645435245No Hit
TCGGTCAAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC570.1890923566878981No Hit
TCGGTCAAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCC450.14928343949044587No Hit
TCGGTAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC440.1459660297239915No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA430.14264861995753714No Hit
TCGGTCAAACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAAC410.13601380042462843No Hit
TCGGTCAAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACCAC410.13601380042462843No Hit
TCGGTCAAACTGGGATTAGATACCCCAATGTGGGCAAACGTCGATGCGTT380.1260615711252654No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA370.12274416135881104No Hit
TCGGTCAAACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC360.11942675159235669No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA350.11610934182590234No Hit
TCGGTCAAATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC340.11279193205944799No Hit
TCGGTCAAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT330.10947452229299363No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATA320.10615711252653928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGA206.085975E-8295.08
CAACTGG206.085975E-8295.06
TCGGTCA29750.0295.01
GGTCAAC255.18412E-10295.03
CGGTCAA29700.0295.02
GTAATTA108.424196E-4295.0295
ACTGGAT206.085975E-8295.09
CAAACTG29200.0295.06
CGGTAAA108.424196E-4295.02
GGTAAAC108.424196E-4295.03
GGTCAAA29450.0294.999973
TCAAATG157.1706672E-6294.999975
AATGGGA157.1706672E-6294.999978
CAAATGG157.1706672E-6294.999976
AAATGGG157.1706672E-6294.999977
GTCAAAC29400.0293.99664
AAACTGG29200.0293.989727
TCAAACT29450.0292.996585
AACTGGG29300.0291.47618
ACTGGGA29250.0291.470069
Produced by FastQC (version 0.11.5)