FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-136-4-7-5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-136-4-7-5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30997
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG630720.347130367454916No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTATT428213.814240087750425No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATTCCTCTGTC19616.3264186856792595No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG14254.597219085717972No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTATT9102.935767977546214No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATTCCTCT8442.7228441462076978No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATTCCTCT7432.397006161886634No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATTCCTC7402.387327805916702No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC6712.1647256186082524No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAT6462.0840726521921473No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATTCCTC6402.0647159402522828No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG5791.8679227021969866No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT4811.551763073845856No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATTCCTCTG4631.4936929380262607No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATTCCTCTGT4351.403361615640223No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG2840.9162176984869503No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT2740.8839565119205084No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATTC2290.7387811723715199No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATTCCTC2270.7323289350582314No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAT2170.7000677484917895No Hit
AAGCGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA2160.6968416298351454No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTATTC2140.6903893925218569No Hit
AAGCGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTATT1820.5871535955092428No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG1560.5032745104364938No Hit
AAGCGACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA1490.4806916798399845No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG1390.4484304932735426No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT1360.43875213730361007No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT1360.43875213730361007No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTATT1350.4355260186469658No Hit
AAGCGACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTATTCC1340.43229989999032165No Hit
AAGCGACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTATTCCT1300.41939542536374486No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG1270.4097170693938123No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAT1100.354873052230861No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG1050.3387424589476401No Hit
AAGCGACTGGGATTAGATACCCCATGCTAGAGGAGCCTGTTCTATTCCTC1020.32906410297770755No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG990.31938574700777494No Hit
AAGCGACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTATT940.30325515372455397No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATTCCTCT920.2968029164112656No Hit
AAGCGACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGGT920.2968029164112656No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTATT920.2968029164112656No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG900.29035067909797724No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTATTCCT880.2838984417846888No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATTCCTCT860.27744620447140045No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATTCC820.26454172984482366No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTAT820.26454172984482366No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG820.26454172984482366No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTATTC800.25808949253153535No Hit
AAGCGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTATTCC760.24518501790495853No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG760.24518501790495853No Hit
AAGCGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTATTCCT710.22905442462173758No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG710.22905442462173758No Hit
AAGCGACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTATTCCTC710.22905442462173758No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAT680.21937606865180503No Hit
AAGCGACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTATT660.2129238313385166No Hit
AAGCGACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTATTCCT620.20001935671193988No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTATTC600.19356711939865148No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGTGACCTAGAGGAGCCTGT580.1871148820853631No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTTCCTCTGTCTCTTATACA560.18066264477207472No Hit
AAGCGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTATTC560.18066264477207472No Hit
AAGCGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTATTC550.1774365261154305No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATTCCTC550.1774365261154305No Hit
AAGCGACTGGGATTAGATACCCCGAGCAACCTAGAGGAGCCTGTTCTATT530.17098428880214214No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTTCCTCTGTCTCTTATACA520.16775817014549793No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG510.16453205148885378No Hit
AAGCGACTGGGATTAGATACCCCAAATCTAGAGGAGCCTGTTCTATTCCT510.16453205148885378No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT490.15807981417556538No Hit
AAGCGACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTATTCC490.15807981417556538No Hit
AAGCGACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTCTA460.1484014582056328No Hit
AAGCGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTATTCCT460.1484014582056328No Hit
AAGCGACTGGGATTAGATACCCCACGGAGATCGAGGGCCTAGAGGAGCCT440.1419492208923444No Hit
AAGCGACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTATTCCTCTG420.13549698357905604No Hit
AAGCGACTGGGATTAGATACCCCCACGAGATCTAGAGGAGCCTGTTCTAT390.12581862760912346No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT380.12259250895247926No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC380.12259250895247926No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT370.11936639029583508No Hit
AAGCGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC360.11614027163919088No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG360.11614027163919088No Hit
AAGCGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTATTC360.11614027163919088No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTATTCCT350.11291415298254669No Hit
AAGCGACTGGGATTAGATACCCCCAGACATAGAGGAGCCTGTTCTATTCC340.10968803432590252No Hit
AAGCGACTGGGATTAGATACCCCAACATAGAGGAGCCTGTTCTATTCCTC330.1064619156692583No Hit
AAGCGACTGGGATTAGATACCCCTGTCTCTTATACACATCTAGAGGAGCC330.1064619156692583No Hit
AAGCGACTGGGATTAGATACCCCCACCCTAGAGGAGCCTGTTCTATTCCT320.10323579701261414No Hit
AAGCGACTGGGATTAGATACCCCTCAGAGCCCACGAGACCTAGAGGAGCC310.10000967835596994No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTGTCTCTTATACACATCCTA310.10000967835596994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTG30700.0295.000033
TGGGATA255.18412E-10295.08
GACTGGG30550.0295.05
CTGGATT157.1726245E-6295.07
GGGATAG206.088703E-8295.09
ACTGGAT157.1726245E-6295.06
CGACTGG30750.0294.520364
CTGGGAT30550.0294.517187
TGGGATT30300.0294.513188
AGCGACT30850.0294.043762
ACTGGGA30600.0294.035986
GGGATTA30400.0294.029639
AAGCGAC31200.0291.217961
GACTGGA202.260807E-5221.255
TGGATTA301.1383856E-4147.58
CCCTGAG150.00437335659.00000420-24
CCTTCCT257.80238E-659.00000430-34
CCCCTGA150.00437335659.00000420-24
GGTACCT150.00437335659.00000435-39
TAAAGTC150.00437335659.00000435-39
Produced by FastQC (version 0.11.5)