FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-135-4-7-5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-135-4-7-5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30810
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACC435014.118792599805257No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG404713.135345666991235No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCTGT30179.792275235313209No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTACC15374.988640051931191No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACTCC15274.9561830574488805No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCACTC10263.3300876338851024No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG8262.6809477442388836No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTACC7812.5348912690684844No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCACTCC6882.233041220382993No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCACTC6121.9863680623174296No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCCTG4951.6066212268743916No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC4581.486530347289841No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCACTCCT4531.4703018500486855No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAC3831.2431028886725088No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAC3671.1911716975008113No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT3461.1230120090879585No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACCA3411.106783511846803No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG3331.0808179162609541No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT3040.9866926322622526No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACCACT3010.9769555339175593No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAC2080.6751054852320675No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT1870.6069457968192146No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACCA1680.5452775073028238No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1680.5452775073028238No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCACTC1640.5322947095098993No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACCA1620.5258033106134372No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCACTCC1620.5258033106134372No Hit
AAGCGACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCCT1600.519311911716975No Hit
AAGCGACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTACCAC1340.4349237260629666No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTACC1300.42194092827004215No Hit
AAGCGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG1280.41544952937358No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTAC1260.4089581304771178No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG1190.38623823433950016No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACCAC1150.3732554365465758No Hit
AAGCGACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTACCAC1130.3667640376501136No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACCACTCC1130.3667640376501136No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCACTC1100.35702693930542034No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT1090.35378123985718923No Hit
AAGCGACTGGGATTAGATACCCCTCTTATCACATCTGACGCTAGAGGAGC1060.34404414151249596No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAC1040.33755274261603374No Hit
AAGCGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTACC1020.3310613437195716No Hit
AAGCGACTGGGATTAGATACCCCGACGACTAGAGGAGCCTGTTCTACCAC1020.3310613437195716No Hit
AAGCGACTGGGATTAGATACCCCTGACGCGACGACTAGAGGAGCCTGTTC1000.32456994482310936No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG970.3148328464784161No Hit
AAGCGACTGGGATTAGATACCCCCGTACGTGCAGGGAGGGTACCTTGAGG930.30185004868549176No Hit
AAGCGACTGGGATTAGATACCCCATGCTAGAGGAGCCTGTTCTACCACTC910.29535864978902954No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACCACT900.2921129503407984No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAC830.26939305420318077No Hit
AAGCGACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTCTA810.2629016553067186No Hit
AAGCGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTACCA800.2596559558584875No Hit
AAGCGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTACCACTCC800.2596559558584875No Hit
AAGCGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTACCA800.2596559558584875No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACCACTCC780.25316455696202533No Hit
AAGCGACTGGGATTAGATACCCCACGAGCTAGAGGAGCCTGTTCTACCAC760.24667315806556314No Hit
AAGCGACTGGGATTAGATACCCCGTGCTAGAGGAGCCTGTTCTACCACTC740.24018175916910092No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCACTCC730.23693605972086984No Hit
AAGCGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACCACT720.23369036027263873No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG680.2207075624797144No Hit
AAGCGACTGGGATTAGATACCCCCGCCACCTAGAGGAGCCTGTTCTACCA650.21097046413502107No Hit
AAGCGACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTACCACT640.20772476468679No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTACCA610.19798766634209672No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG600.19474196689386564No Hit
AAGCGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG580.18825056799740345No Hit
AAGCGACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTACCACTC580.18825056799740345No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG570.18500486854917234No Hit
AAGCGACTGGGATTAGATACCCCCAGACTAGAGGAGCCTGTTCTACCACT560.18175916910094125No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG520.16877637130801687No Hit
AAGCGACTGGGATTAGATACCCCAAGCCCGACTAGAGGAGCCTGTTCTAC520.16877637130801687No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCACTCC510.1655306718597858No Hit
AAGCGACTGGGATTAGATACCCCTGTGTATAAGAGGAGCCTGTTCTACCA510.1655306718597858No Hit
AAGCGACTGGGATTAGATACCCCCCTCCGACAATCGCGATTCGAACTGGA500.16228497241155468No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG480.15579357351509251No Hit
AAGCGACTGGGATTAGATACCCCCGCTAGAGGAGCCTGTTCTACCACTCC480.15579357351509251No Hit
AAGCGACTGGGATTAGATACCCCGACTTAGAGGAGCCTGTTCTACCACTC480.15579357351509251No Hit
AAGCGACTGGGATTAGATACCCCGAGATCTAGAGGAGCCTGTTCTACCAC460.1493021746186303No Hit
AAGCGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTACCACT430.13956507627393705No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG430.13956507627393705No Hit
AAGCGACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGAGC420.13631937682570594No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG400.12982797792924375No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT370.12009087958455046No Hit
AAGCGACTGGGATTAGATACCCCGAGATCGAAAGAGCGCTAGAGGAGCCT360.11684518013631937No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG360.11684518013631937No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC360.11684518013631937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGG30650.0294.999974
AGCGACT30750.0294.520362
GACTGGG30550.0294.517185
CTGGGAT30450.0294.51567
ACTGGGA30600.0294.035926
TGGGATT30450.0294.03128
GGGATTA30350.0294.0289
AAGCGAC30950.0293.093721
GCGACTG30900.0293.090643
TGGATTA150.0028363054196.666678
GACTGGA150.0028363054196.666675
CTGGATT150.0028363054196.666677
GGGATTG150.0028363054196.666679
CCCCTGA452.7284841E-1159.00000420-24
CTCGCCA4250.059.00000465-69
ATGCCTA1250.059.00000425-29
CGGGTAG201.8424298E-459.020-24
CCGGGTA201.8424298E-459.020-24
TACCCCG4850.059.015-19
TACCCCC2350.059.015-19
Produced by FastQC (version 0.11.5)