FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-134-5-7-5-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-134-5-7-5-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30200
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG969832.11258278145696No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG22677.506622516556291No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGG21737.195364238410596No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGCTG16375.420529801324504No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAATCG10173.3675496688741724No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC9793.2417218543046356No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG8892.9437086092715234No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT5081.6821192052980134No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGG4921.629139072847682No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG4391.4536423841059603No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGGAATCG3871.281456953642384No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGG3751.2417218543046358No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATCGCT3511.1622516556291391No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGGAATC3501.1589403973509933No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT2760.9139072847682119No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG2610.8642384105960265No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG2300.7615894039735099No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT2190.7251655629139072No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG2150.7119205298013245No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGGAATC2020.6688741721854304No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGGAATCGC1990.6589403973509934No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGGAA1820.6026490066225165No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG1770.5860927152317881No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG1660.5496688741721855No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG1540.509933774834437No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAG1420.47019867549668876No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGG1370.45364238410596025No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGGAATC1320.43708609271523186No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG1260.41721854304635764No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG1250.4139072847682119No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG1230.4072847682119205No Hit
AAGCGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG1180.39072847682119205No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG1060.3509933774834437No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGGAAT920.304635761589404No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGGAATCG900.2980132450331126No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG850.2814569536423841No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG830.27483443708609273No Hit
AAGCGACTGGGATTAGATACCCCTGACGCATAGAGGAGCCTGTTCTAGGA730.24172185430463575No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT710.23509933774834438No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGGA680.2251655629139073No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG640.2119205298013245No Hit
AAGCGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTGT590.19536423841059603No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC590.19536423841059603No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGGAATCG580.19205298013245034No Hit
AAGCGACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTAGG570.18874172185430466No Hit
AAGCGACTGGGATTAGATACCCCCACGACCTAGAGGAGCCTGTTCTAGGA550.18211920529801326No Hit
AAGCGACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTAGG540.17880794701986755No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT490.1622516556291391No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT480.15894039735099338No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT480.15894039735099338No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG460.152317880794702No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGGA450.1490066225165563No Hit
AAGCGACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCTG450.1490066225165563No Hit
AAGCGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGGAAT440.1456953642384106No Hit
AAGCGACTGGGATTAGATACCCCACGAACCTAGAGGAGCCTGTTCTAGGA440.1456953642384106No Hit
AAGCGACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGGAATC430.1423841059602649No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAGGA430.1423841059602649No Hit
AAGCGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAGGA400.13245033112582782No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT380.12582781456953643No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATGA380.12582781456953643No Hit
AAGCGACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAGGA370.12251655629139074No Hit
AAGCGACTGGGATTAGATACCCCACCCCTAGAGGAGCCTGTTCTAGGAAT350.11589403973509935No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACAG350.11589403973509935No Hit
AAGCGACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGGAA340.11258278145695365No Hit
AAGCGACTGGGATTAGATACCCCAATGTCTAGAGGAGCCTGTTCTAGGAA330.10927152317880796No Hit
AAGCGACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGAGC330.10927152317880796No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG330.10927152317880796No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGGA320.10596026490066225No Hit
AAGCGACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA320.10596026490066225No Hit
AAGCGACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGGAATC310.10264900662251657No Hit
AAGCGACTGGGATTAGATACCCCGCGAGACCTAGAGGAGCCTGTTCTAGG310.10264900662251657No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG108.429009E-4294.945374
GCGACTG30000.0294.945373
GCTGGGA108.429009E-4294.945375
GCGCTGG108.429009E-4294.945373
TGGATTA157.176208E-6294.945378
GACTGGG29800.0294.945375
AGCGCTG108.429009E-4294.945372
ACTGGGA29750.0294.945376
CGACTGG29950.0294.945344
AAGCGAC30050.0294.45461
AGCGACT30050.0294.45462
CTGGGAT29900.0293.958927
GGGATTA29900.0293.958929
TGGGATT29950.0293.468178
CTGGATT202.2619352E-5221.209037
AAGCGCT150.0028376048196.630251
ACTGGAT255.5074503E-5176.967226
GACTGGA403.5803256E-4110.6045155
CAGCCCT406.039045E-1059.08690330-34
AATCTTG750.059.08690340-44
Produced by FastQC (version 0.11.5)