FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-133-4-7-5-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-133-4-7-5-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30566
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGC450714.745141660668718No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG393812.883596152587842No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAACT26218.574887129490282No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTGAGA16015.23784597264935No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGAGAA8872.901917162860695No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG8702.846299810246679No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGC8122.6565464895635675No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGAAC7952.600929136949552No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG7832.5616698292220113No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT7392.41771903422103No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGC6822.231237322515213No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTGAG6372.0840149185369365No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC4821.5769155270562063No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAG4241.3871622063730942No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCTGAG4191.370804161486619No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGC3821.2497546293267028No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTGAGA3301.0796309625073612No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG3030.9912975201203952No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT2970.9716678662566249No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGCT2650.8669763789831839No Hit
AAGCGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAGCT2540.8309886802329386No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGCTGAGA2480.8113590263691683No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGCTGAG2430.7950009814826933No Hit
AAGCGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG2340.7655565006870378No Hit
AAGCGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA2300.7524700647778578No Hit
AAGCGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAGC2020.6608650134135968No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG1810.5921612248904011No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1690.5529019171628606No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGCTGAGA1670.5463586992082706No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGCT1600.5234574363672054No Hit
AAGCGACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGCTGA1580.5169142184126153No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGCTG1570.5136426094353203No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGCT1510.4940129555715501No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG1280.4187659490937643No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAGCT1250.40895112216187923No Hit
AAGCGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGCTGAGAA1180.386049859320814No Hit
AAGCGACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTAGCTGA1090.35660537852515867No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGCTGA1060.34679055159327354No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCTGAG1050.34351894261597854No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG1050.34351894261597854No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGCT1030.3369757246613885No Hit
AAGCGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGCT990.32388928875220835No Hit
AAGCGACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTAGC980.3206176797749133No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT970.3173460707976183No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG950.31080285284302817No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG870.2846299810246679No Hit
AAGCGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGCTGA850.27808676307007785No Hit
AAGCGACTGGGATTAGATACCCCCACCCTAGAGGAGCCTGTTCTAGCTGA840.27481515409278284No Hit
AAGCGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAGCTG810.2650003271608977No Hit
AAGCGACTGGGATTAGATACCCCTGACGCTGCTAGAGGAGCCTGTTCTAG800.2617287181836027No Hit
AAGCGACTGGGATTAGATACCCCCCAACCTAGAGGAGCCTGTTCTAGCTG760.24864228227442253No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGCTGA700.22901262841065234No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGCTGA690.2257410194333573No Hit
AAGCGACTGGGATTAGATACCCCAAACTAGAGGAGCCTGTTCTAGCTGAG660.21592619250147224No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG660.21592619250147224No Hit
AAGCGACTGGGATTAGATACCCCGGCAGACCTAGAGGAGCCTGTTCTAGC660.21592619250147224No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAGCT630.20611136556958712No Hit
AAGCGACTGGGATTAGATACCCCCCATCTAGAGGAGCCTGTTCTAGCTGA620.2028397565922921No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG620.2028397565922921No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG610.19956814761499705No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGCTGAGA550.17993849375122686No Hit
AAGCGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTGT540.17666688477393183No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAGCT540.17666688477393183No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG530.17339527579663677No Hit
AAGCGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAGC530.17339527579663677No Hit
AAGCGACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAGC500.16358044886475168No Hit
AAGCGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGCTGA490.16030883988745664No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG470.15376562193286658No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAGC460.15049401295557155No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT440.14395079500098149No Hit
AAGCGACTGGGATTAGATACCCCGAAACCTAGAGGAGCCTGTTCTAGCTG420.13740757704639142No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG390.12759275011450633No Hit
AAGCGACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGAGC380.12432114113721127No Hit
AAGCGACTGGGATTAGATACCCCCATGTGTGTGTGTGTGTGTGTGTCTAG370.12104953215991625No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG370.12104953215991625No Hit
AAGCGACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTAGCT370.12104953215991625No Hit
AAGCGACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGCTG360.11777792318262122No Hit
AAGCGACTGGGATTAGATACCCATAGAGGAGCCTGTTCTAGCTGAGAACT310.10141987829614604No Hit
AAGCGACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA310.10141987829614604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT30200.0295.07
GGGATTG108.4248127E-4295.09
ACTGGGA30200.0295.06
TGGGATA157.1717186E-6294.999978
GGGATAG157.1717186E-6294.999979
GACTGGG30250.0294.51245
TGGGATT30100.0294.509958
GGGATTA30000.0294.508339
CGACTGG30450.0294.03124
GCGACTG30650.0293.075073
AAGCGAC30800.0292.60551
AGCGACT30850.0291.653172
GTGACGA150.0028361934196.66666295
CTGGATT255.5040095E-5177.07
ACTGGAT255.5040095E-5177.06
GACTGGA301.13824266E-4147.499985
TGGATTA352.1030648E-4126.428588
AGTCCCT1250.059.00000425-29
AGCCCTA4950.059.00000430-34
TCCCTAG1250.059.00000425-29
Produced by FastQC (version 0.11.5)