FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-132-5-5-7-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-132-5-5-7-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29762
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC588319.76681674618641No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA376412.646999529601505No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAA20356.837578119750017No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTGA14024.7107049257442375No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT10913.6657482696055372No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA7262.439352194072979No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTG6532.1940729789664672No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA5541.8614340434110612No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGA5521.8547140649149922No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT5441.827834150930717No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC5291.777434312210201No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT4321.4515153551508635No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT4111.380955580942141No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTGA3951.3271957529735905No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGAG3551.1927961830522142No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT3511.1793562260600765No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT3501.1759962368120422No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT3081.034876688394597No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA2890.9710368926819434No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCTG2750.9239970432094617No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGC2650.8903971507291176No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC2610.87695719373698No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGCTG1910.6417579463745716No Hit
CTGGATGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT1690.5678381829178147No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGCT1660.5577582151737115No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT1540.5174383441972985No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAG1540.5174383441972985No Hit
CTGGATGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGCTGA1520.5107183657012297No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1410.4737584839728513No Hit
CTGGATGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA1270.42671863450036956No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAG1120.3763187957798535No Hit
CTGGATGACTGGGATTAGATACCCCCCGAGACCTAGAGGAGCCTGTTCTA1110.37295880653181906No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCTG1080.3628788387877159No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT1040.34943888179557825No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT970.32591895705933743No Hit
CTGGATGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT920.3091190108191654No Hit
CTGGATGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAG910.305759021571131No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC880.29567905382702775No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT870.29231906457899337No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT830.27887910758685575No Hit
CTGGATGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGCTGA820.2755191183388213No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC820.2755191183388213No Hit
CTGGATGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA770.25871917209864925No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT720.24191922585847728No Hit
CTGGATGACTGGGATTAGATACCCCTGACCGACTAGAGGAGCCTGTTCTA700.2351992473624084No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT680.22847926886633962No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT680.22847926886633962No Hit
CTGGATGACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGCTG670.22511927961830522No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGCTGA670.22511927961830522No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAG650.2183993011222364No Hit
CTGGATGACTGGGATTAGATACCCCGCAGACCTAGAGGAGCCTGTTCTAG640.215039311874202No Hit
CTGGATGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGCT640.215039311874202No Hit
CTGGATGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC620.20831933337813321No Hit
CTGGATGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGCTGA610.20495934413009875No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGCTGA590.19823936563402997No Hit
CTGGATGACTGGGATTAGATACCCCATGTGCCTCTAGAGGAGCCTGTTCT590.19823936563402997No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT580.19487937638599556No Hit
CTGGATGACTGGGATTAGATACCCCCACTCCGCTAGAGGAGCCTGTTCTA560.18815939788992675No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGCTGA560.18815939788992675No Hit
CTGGATGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAG550.18479940864189234No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG550.18479940864189234No Hit
CTGGATGACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTA530.17807943014582353No Hit
CTGGATGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAG530.17807943014582353No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG530.17807943014582353No Hit
CTGGATGACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGCTG470.1579194946576171No Hit
CTGGATGACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGA470.1579194946576171No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCT460.1545595054095827No Hit
CTGGATGACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTA460.1545595054095827No Hit
CTGGATGACTGGGATTAGATACCCCCACGGGACCTAGAGGAGCCTGTTCT450.15119951616154828No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT440.14783952691351387No Hit
CTGGATGACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGCT440.14783952691351387No Hit
CTGGATGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGCT440.14783952691351387No Hit
CTGGATGACTGGGATTAGATACCCCAACCCACCTAGAGGAGCCTGTTCTA420.14111954841744506No Hit
CTGGATGACTGGGATTAGATACCCCCGACCAACCTAGAGGAGCCTGTTCT420.14111954841744506No Hit
CTGGATGACTGGGATTAGATACCCCACGAACTAGAGGAGCCTGTTCTAGC410.13775955916941066No Hit
CTGGATGACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTT400.13439956992137625No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA390.13103958067334184No Hit
CTGGATGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGCT380.12767959142530744No Hit
CTGGATGACTGGGATTAGATACCCCCACGAATAGAGGAGCCTGTTCTAGC350.1175996236812042No Hit
CTGGATGACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTAG340.11423963443316981No Hit
CTGGATGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGCTGAG330.1108796451851354No Hit
CTGGATGACTGGGATTAGATACCCCGAAACCTAGAGGAGCCTGTTCTAGC310.10415966668906661No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGAT310.10415966668906661No Hit
CTGGATGACTGGGATTAGATACCCCGACGAACCTAGAGGAGCCTGTTCTA300.10079967744103219No Hit
CTGGATGACTGGGATTAGATACCCCAATGTCAGACTAGAGGAGCCTGTTC300.10079967744103219No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA300.10079967744103219No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGG108.423702E-4295.06
GACTGGG29250.0295.07
CTGGATG29550.0295.01
TGACTGG29300.0295.06
CTGGGAT29350.0294.497449
ACTGGGA29350.0294.497448
TGGATGA29700.0293.51012
GGATGAC29650.0293.50763
GATGACT29600.0293.006744
ATGACTG29700.0291.523565
ACGGGAT150.0028358204196.666668
ATACTGG150.0028358204196.666665
CGGGATT200.0067049717147.59
GACGGGA200.0067049717147.57
TGGATAC200.0067049717147.52
CTGGATA200.0067049717147.51
AAACTTT452.7284841E-1159.00000440-44
AACCGCT900.059.00000465-69
GATACCC29550.059.00000415-19
AACTTTG452.7284841E-1159.00000440-44
Produced by FastQC (version 0.11.5)