FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-131-6-7-5-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-131-6-7-5-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28732
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1710259.52248364193235No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG11994.173047473200612No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC9043.146317694556592No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG7132.481553668383684No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG4791.667130725323681No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG4671.6253654461923988No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG4481.5592370875678685No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG4251.479186969232911No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT3891.3538911318390645No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT3791.3190867325629958No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG3441.197271335096756No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG3391.1798691354587219No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG2951.0267297786440206No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG2951.0267297786440206No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG2860.9954058192955589No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG2690.9362383405262424No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATAC2190.7622163441459001No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG2120.7378532646526521No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG1980.6891271056661562No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG1390.4837811499373521No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTACGCCCTAAACGATGATCG1290.4489767506612836No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACACAAATAG1280.44549631073367674No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTCAGCCCTAAACTTCAACAG990.3445635528330781No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA960.33412223305025757No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG940.32716135319504386No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTTATGC880.3062787136294028No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT760.26451343449812054No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC730.2540721147153No Hit
AAGCGACTGGGATTAGATACCCCAGAGAACAATGATAGACGTCAGCTTGT640.22274815536683837No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT480.16706111652512878No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTATA480.16706111652512878No Hit
AAGCGACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT390.13573715717666712No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACTGT390.13573715717666712No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG370.12877627732145344No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG370.12877627732145344No Hit
AAGCGACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT310.10789363775581234No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCACTAAACCTCAACAG310.10789363775581234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTG28400.0295.03
TCTGGGA108.422152E-4295.06
GTCTGGG108.422152E-4295.05
GTTTTAA108.422152E-4295.0295
GCGTCTG108.422152E-4295.03
AAGCGTC108.422152E-4295.01
GGGATTT108.422152E-4295.09
AGCGTCT108.422152E-4295.02
AGCGACT28400.0295.02
CGTCTGG108.422152E-4295.04
CTGGGAT28450.0294.481547
CGACTGG28450.0294.481544
ACTGGGA28350.0294.47976
GACTGGG28450.0293.96315
TGGGATT28500.0293.447368
GGGATTA28450.0293.444649
AAGCGAC28700.0292.944271
AAGAGGA150.0028352996196.66667295
CGTGTCG150.0028352996196.66667295
TCATGTC200.0067037437147.5295
Produced by FastQC (version 0.11.5)