FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-130-5-1-3-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-130-5-1-3-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29025
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC882330.397932816537466No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA20477.0525409130060295No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT19666.773471145564169No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACT15625.381567614125753No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT8432.904392764857881No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT7942.73557278208441No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA6342.1843238587424634No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGC6292.1670973298880276No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT5591.925925925925926No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCAC4531.5607235142118863No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAG4141.426356589147287No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC3841.322997416020672No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA3341.1507321274763136No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT3141.0818260120585703No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT2880.992248062015504No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAGC2830.9750215331610681No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATAAGCA2770.954349698535745No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATA2640.909560723514212No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC2540.8751076658053402No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATAAG2370.8165374677002584No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA2290.7889750215331611No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT2060.7097329888027563No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1930.664944013781223No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT1680.5788113695090439No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT1600.5512489233419465No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC1460.5030146425495262No Hit
ATCGGTTACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAT1420.4892334194659776No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTATAA1410.48578811369509045No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT1220.42032730404823426No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1060.3652024117140396No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT1040.35831180017226527No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT1040.35831180017226527No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT1030.35486649440137813No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATAAG980.3376399655469423No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATAAGC860.2962962962962963No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG850.29285099052540914No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT850.29285099052540914No Hit
ATCGGTTACTGGGATTAGATACCCCCCTAACACTAGAGGAGCCTGTTCTA830.2859603789836348No Hit
ATCGGTTACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTATA780.268733850129199No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC740.2549526270456503No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG630.21705426356589144No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAT630.21705426356589144No Hit
ATCGGTTACTGGGATTAGATACCCCAAGCTAGAGGAGCCTGTTCTATAAG590.20327304048234282No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT590.20327304048234282No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA580.19982773471145565No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATAAGCA560.1929371231696813No Hit
ATCGGTTACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATAAGC550.18949181739879414No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT530.1826012058570198No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATAAGC520.17915590008613264No Hit
ATCGGTTACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA500.17226528854435832No Hit
ATCGGTTACTGGGATTAGATACCCCCCGGCAATTACCGTGTAGTCGCCAA500.17226528854435832No Hit
ATCGGTTACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTA500.17226528854435832No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTATAA490.16881998277347116No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAT490.16881998277347116No Hit
ATCGGTTACTGGGATTAGATACCCCAATATGCTAGAGGAGCCTGTTCTAT480.165374677002584No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAT480.165374677002584No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTT470.16192937123169682No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAT460.15848406546080965No Hit
ATCGGTTACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTATAAGC450.15503875968992248No Hit
ATCGGTTACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTATAA430.14814814814814814No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATAAG420.14470284237726097No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC390.1343669250645995No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTA390.1343669250645995No Hit
ATCGGTTACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA390.1343669250645995No Hit
ATCGGTTACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTATAAG390.1343669250645995No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCT380.13092161929371232No Hit
ATCGGTTACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTATAAGCA370.12747631352282515No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATAAGC370.12747631352282515No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAAT370.12747631352282515No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC370.12747631352282515No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT360.124031007751938No Hit
ATCGGTTACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA340.11714039621016366No Hit
ATCGGTTACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGA340.11714039621016366No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTATA330.1136950904392765No Hit
ATCGGTTACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGT320.11024978466838932No Hit
ATCGGTTACTGGGATTAGATACCCCACGTCTAGAGGAGCCTGTTCTATAA310.10680447889750216No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTATAA310.10680447889750216No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAT300.10335917312661498No Hit
ATCGGTTACTGGGATTAGATACCCCAGGCCCACGAGACCTAGAGGAGCCT300.10335917312661498No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGG108.4226066E-4295.06
ACCGGGA108.4226066E-4295.08
TTACCGG108.4226066E-4295.06
TTACTGG28450.0295.06
TCGGTTA28800.0295.02
TCGGTGA108.4226066E-4295.02
GGTTACT28550.0295.04
GGTTACC108.4226066E-4295.04
ATCGGTG108.4226066E-4295.01
TACCGGG108.4226066E-4295.07
GACTGGG108.4226066E-4295.07
CCGGGAT108.4226066E-4295.09
CGGTTAC28650.0295.03
GTTACTG28550.0295.05
GGTACTG108.4226066E-4295.04
GTTATGG108.4226066E-4295.05
GTTACCG108.4226066E-4295.05
TATGGGA108.4226066E-4295.07
CTGGATT303.6379788E-12294.999979
ATCGGTT28850.0294.488741
Produced by FastQC (version 0.11.5)