FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-13-4-5-7-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-13-4-5-7-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11657
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC196416.848245689285406No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA166814.308998884790256No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAAGTCTCCT9368.029510165565755No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAAGTCT5264.5123101998799005No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT3893.3370506991507245No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAAGTC3012.5821394870035173No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC2842.4363043664750794No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2762.3676760744616967No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT2652.273312172943296No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAAGTC2392.050270223899803No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAAGTCTC2161.852963884361328No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAAGTCT1631.398301449772669No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT1541.3210946212576136No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT1491.2782019387492494No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1341.1495238912241572No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1171.0036887706957194No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT1000.8578536501672814No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAAGTC950.8149609676589173No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC880.7549112121472077No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAAGT770.6605473106288068No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAAGTCTCC750.6433902376254611No Hit
CTGGATGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT740.6348117011237883No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA730.6262331646221155No Hit
CTGGATGACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCC720.6176546281204427No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAG710.6090760916187699No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAA670.5747619456120786No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAA550.47181950759200475No Hit
CTGGATGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAAGT540.463240971090332No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC530.4546624345886592No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT510.4375053615853135No Hit
CTGGATGACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTC470.40319121557862225No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT460.39461267907694947No Hit
CTGGATGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA450.3860341425752767No Hit
CTGGATGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAAGT450.3860341425752767No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAAGTCT440.37745560607360384No Hit
CTGGATGACTGGGATTAGATACCCCCAGCACCTAGAGGAGCCTGTTCTAA440.37745560607360384No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA440.37745560607360384No Hit
CTGGATGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC410.35171999656858544No Hit
CTGGATGACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAAG390.3345629235652398No Hit
CTGGATGACTGGGATTAGATACCCCGACAACCTAGAGGAGCCTGTTCTAA360.30882731406022135No Hit
CTGGATGACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAAG360.30882731406022135No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT350.3002487775585485No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC350.3002487775585485No Hit
CTGGATGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCT350.3002487775585485No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTG350.3002487775585485No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT340.2916702410568757No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTAAGT340.2916702410568757No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAAGTCT330.2830917045552029No Hit
CTGGATGACTGGGATTAGATACCCCGCCAACTAGAGGAGCCTGTTCTAAG330.2830917045552029No Hit
CTGGATGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA330.2830917045552029No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA300.2573560950501844No Hit
CTGGATGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAAGTCT300.2573560950501844No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAA290.24877755854851163No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT280.2401990220468388No Hit
CTGGATGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAA280.2401990220468388No Hit
CTGGATGACTGGGATTAGATACCCCTCTATACACATTACGCTAGAGGAGC280.2401990220468388No Hit
CTGGATGACTGGGATTAGATACCCCACGAACTAGAGGAGCCTGTTCTAAG270.231620485545166No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT270.231620485545166No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAAGTCT260.22304194904349317No Hit
CTGGATGACTGGGATTAGATACCCCAAGCTAGAGGAGCCTGTTCTAAGTC260.22304194904349317No Hit
CTGGATGACTGGGATTAGATACCCCCTGTCTCTTAAACCTAGAGGAGCCT250.21446341254182036No Hit
CTGGATGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAAGT240.20588487604014755No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAA240.20588487604014755No Hit
CTGGATGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAAGT230.19730633953847473No Hit
CTGGATGACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGC220.18872780303680192No Hit
CTGGATGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT210.1801492665351291No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT210.1801492665351291No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT200.1715707300334563No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC200.1715707300334563No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA190.16299219353178349No Hit
CTGGATGACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAAGT180.15441365703011067No Hit
CTGGATGACTGGGATTAGATACCCCAGCAGACCTAGAGGAGCCTGTTCTA170.14583512052843786No Hit
CTGGATGACTGGGATTAGATACCCCGAGATCGGAAGAGCGCTAGAGGAGC170.14583512052843786No Hit
CTGGATGACTGGGATTAGATACCCCCGCCAAACCTAGAGGAGCCTGTTCT160.13725658402676502No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC150.1286780475250922No Hit
CTGGATGACTGGGATTAGATACCCCCATCTAGAGGAGCCTGTTCTAAGTC150.1286780475250922No Hit
CTGGATGACTGGGATTAGATACCCAGACCTAGAGGAGCCTGTTCTAAGTC150.1286780475250922No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT150.1286780475250922No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT140.1200995110234194No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACTAGACCTAGAGGAGCCT140.1200995110234194No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT130.11152097452174659No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAAC130.11152097452174659No Hit
CTGGATGACTGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA120.10294243802007377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGAC11550.0295.03
CTGGGAT11400.0295.09
GATGACT11550.0295.04
GACTGGG11400.0295.07
ACTGGGA11400.0295.08
CTGGATG11650.0293.733921
TGGATGA11650.0293.733922
TGACTGG11550.0293.722966
ATGACTG11650.0292.46785
CTGGATT150.0028137502196.666679
GACTGGA150.0028137502196.666677
GTCAACA200.0066529224147.49998295
ACTGGAT200.0066529224147.499988
TATGCTT1800.059.00000425-29
CCGCCAG900.059.00000470-74
TAGCCCT1900.059.00000430-34
CAATTGA452.5465852E-1159.00000460-64
TATCCGC900.059.00000465-69
ACCCCTG452.5465852E-1159.00000420-24
ACCCCTA950.059.00000420-24
Produced by FastQC (version 0.11.5)