FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-129-5-1-3-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-129-5-1-3-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28957
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC867029.940946921297098No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA25288.730186138066788No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT18226.2920882688123765No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGA15915.494353696860863No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT8162.817971474945609No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTG7602.6245812756846356No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT6612.282695030562558No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT5001.7266982076872606No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA4231.4607866837034225No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC4111.4193459267189281No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAG3941.360638187657561No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA3521.2155955382118313No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATT3471.1983285561349588No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGATT3221.1119936457505957No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGATTG3211.1085402493352212No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT2911.0049383568739856No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT2901.001484960458611No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT2530.8737092930897538No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAG2440.8426287253513831No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC2140.7390268328901475No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC2030.7010394723210277No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC1830.6319715440135374No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT1730.5974375798597921No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGATTGA1660.5732638049521704No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAG1540.5318230479676762No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGATTG1510.5214628587215527No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT1480.511102669475429No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT1430.4938356873985565No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1390.48002210173705834No Hit
ATCGGTTACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAG1350.4662085160755603No Hit
ATCGGTTACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC1280.44203474116793867No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT1220.42131436267569156No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT1090.37642020927582276No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTAGA1010.3487930379528266No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT1000.3453396415374521No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGA1000.3453396415374521No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAG930.32116586662983043No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT870.3004454881375833No Hit
ATCGGTTACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAG860.2969920917222088No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT840.29008529889145973No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGATT830.2866319024760852No Hit
ATCGGTTACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGATTG830.2866319024760852No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT830.2866319024760852No Hit
ATCGGTTACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGATTG790.2728183168145871No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGATTGA790.2728183168145871No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG780.2693649203992126No Hit
ATCGGTTACTGGGATTAGATACCCCACGAACTAGAGGAGCCTGTTCTAGA750.2590047311530891No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT730.25209793832234No Hit
ATCGGTTACTGGGATTAGATACCCCAACGAGACCTAGAGGAGCCTGTTCT700.24173774907621645No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA700.24173774907621645No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAG600.20720378492247124No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA600.20720378492247124No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA580.20029699209172222No Hit
ATCGGTTACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCTA570.19684359567634768No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGATT530.18303001001484961No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGAT520.17957661359947508No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGATT500.17266982076872606No Hit
ATCGGTTACTGGGATTAGATACCCCAGGGAGATCGAGGGCCTAGAGGAGC470.16230963152260247No Hit
ATCGGTTACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA440.1519494422764789No Hit
ATCGGTTACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA420.14504264944572987No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAG410.14158925303035536No Hit
ATCGGTTACTGGGATTAGATACCCCGCACGGACCTAGAGGAGCCTGTTCT400.13813585661498082No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGAT400.13813585661498082No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGATTG400.13813585661498082No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG370.12777566736885726No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT350.12086887453810823No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC350.12086887453810823No Hit
ATCGGTTACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA340.11741547812273372No Hit
ATCGGTTACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGATTG340.11741547812273372No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGTGCGGACTAGAGGAGCCTGT330.11396208170735918No Hit
ATCGGTTACTGGGATTAGATACCCCGCCACCTAGAGGAGCCTGTTCTAGA300.10360189246123562No Hit
ATCGGTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA290.10014849604586111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTACT28650.0295.04
CGGTTAC28750.0295.03
TCGGTAC108.422533E-4294.999972
ATCGGTA108.422533E-4294.999971
TCGGTTA28900.0294.489622
ATCGGTT28900.0294.489621
GTTACTG28750.0293.973945
TACTGGG28650.0293.970347
CTGGGAT28550.0293.966749
TTACTGG28750.0293.460886
ACTGGGA28650.0293.45558
GTACTGG150.0028354272196.666675
GGTACTG150.0028354272196.666674
CGGTACT150.0028354272196.666673
CCAGCTT3100.059.00000490-94
CCTGACG452.7284841E-1159.00000420-24
TGCTCGC8500.059.00000465-69
GCTTTAC452.7284841E-1159.000004120-124
CTTCAAC550.059.040-44
AATCTTG800.059.040-44
Produced by FastQC (version 0.11.5)