FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-128-5-1-3-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-128-5-1-3-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28790
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC840529.194164640500176No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA19686.8357068426537No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGC18216.325112886418896No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT17986.245224036123654No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT8172.837790899617923No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT7542.6189649183744357No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA6392.2195206668982284No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACGT5972.0736366794025702No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT4941.715873567210837No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA3721.292115317818687No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC3651.267801319902744No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTGG3531.2261201806182702No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACG3511.219173324070858No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGACGT3121.0837096213963182No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGACG3081.0698159083014935No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGACGTG3081.0698159083014935No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT2840.9864536297325459No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC2670.9274053490795415No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT2670.9274053490795415No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT2610.9065647794373046No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2510.871830496700243No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGACG2330.8093087877735324No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT2220.7711010767627648No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC2210.7676276484890587No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGA2030.705105939562348No Hit
ATCGGTTACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAG1680.5835359499826329No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC1560.5418548106981591No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1450.5036470996873914No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT1390.4828065300451546No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAG1210.4202848211184439No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT1170.40639110802361933No Hit
ATCGGTTACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTA1060.3681833970128517No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAG1060.3681833970128517No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGACGT1030.35776311219173323No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGACGT990.3438693990969086No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT950.32997568600208405No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT950.32997568600208405No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT910.3160819729072594No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT900.3126085446335533No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG870.30218825981243486No Hit
ATCGGTTACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCC870.30218825981243486No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT850.2952414032650226No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT840.29176797499131646No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT690.2396665508857242No Hit
ATCGGTTACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTAGACGTG670.2327196943383119No Hit
ATCGGTTACTGGGATTAGATACCCCAACGAGACCTAGAGGAGCCTGTTCT660.22924626606460577No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGACGT660.22924626606460577No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG650.22577283779089963No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAG650.22577283779089963No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGAC640.22229940951719349No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGACG620.21535255296978115No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT540.18756512678013199No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT510.17714484195901356No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAG510.17714484195901356No Hit
ATCGGTTACTGGGATTAGATACCCCAATCTAGAGGAGCCTGTTCTAGACG500.1736714136853074No Hit
ATCGGTTACTGGGATTAGATACCCCCCAACCTAGAGGAGCCTGTTCTAGA490.17019798541160125No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAG480.1667245571378951No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGAT470.16325112886418897No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA470.16325112886418897No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC470.16325112886418897No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGAC460.15977770059048282No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA440.1528308440430705No Hit
ATCGGTTACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGAC430.14935741576936437No Hit
ATCGGTTACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTC430.14935741576936437No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGACG420.14588398749565823No Hit
ATCGGTTACTGGGATTAGATACCCCCCGCTAGAGGAGCCTGTTCTAGACG410.1424105592219521No Hit
ATCGGTTACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT380.13199027440083363No Hit
ATCGGTTACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA380.13199027440083363No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGACGTG380.13199027440083363No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGGACCTAGAGGAGCCTGTTCTA340.11809656130600903No Hit
ATCGGTTACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGACGT330.11462313303230288No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGAC300.10420284821118443No Hit
ATCGGTTACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA300.10420284821118443No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT290.10072941993747829No Hit
ATCGGTTACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGAC290.10072941993747829No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTA28700.0295.02
ATCGGTT28700.0295.01
CTGGATT255.18412E-10295.09
ACTGGAT255.18412E-10295.08
GGTTACT28700.0294.486054
ACTGGGA28250.0294.477848
CTGGGAT28200.0294.476969
TACTGGG28300.0293.95767
CGGTTAC28850.0293.46623
GTTACTG28700.0293.45825
TTACTGG28600.0293.45286
TACTGGA301.542503E-9245.833347
GGAGATC303.321893E-759.00000425-29
CCAACCT303.321893E-759.00000420-24
AAATCTT950.059.00000440-44
GAGGACC150.00437272559.00000425-29
TATGTTT150.00437272559.00000425-29
GTCGTCT150.00437272559.000004210-214
GGGTACT150.00437272559.00000475-79
CCGCTAT150.00437272559.00000420-24
Produced by FastQC (version 0.11.5)