FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-127-4-7-5-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-127-4-7-5-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28484
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG593720.84328043814071No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACC305210.714787248981885No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCTAGCTGTC18746.579132144361748No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCTAGCT12854.511304592051678No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG12104.2479988765622805No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC6042.1204886954079485No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCTAGCT5932.081870523802837No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTACC5932.081870523802837No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCTAGCTG5691.9976126948462296No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCTAGC4721.6570706361466088No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCTAGCTGT4631.6254739502878808No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG4211.4780227496138183No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT3951.3867434349108272No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCTAGC3581.2568459486027244No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAC3431.2041848055048447No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT3381.1866310911388849No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCTAGC2820.9900294902401349No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACCTA2590.9092824041567196No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT2480.870664232551608No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG2340.8215138323269203No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAC2310.8109816037073445No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTACC2260.7934278893413846No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTACC2100.7372560033703132No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAC1900.6670411459064738No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG1740.6108692599354024No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACCT1670.5862940598230585No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCTAGCT1660.5827833169498666No Hit
AAGCGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTACC1620.5687403454570987No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACCTAGCT1560.5476758882179469No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG1450.5090577166128353No Hit
AAGCGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTACCTA1440.5055469737396433No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTACCTAG1270.44586434489537985No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACCTAG1250.4388428591489959No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG1230.431821373402612No Hit
AAGCGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTACCT1180.4142676590366522No Hit
AAGCGACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTACCTAGCTG1140.4002246875438843No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCTAGC1100.38618171605111645No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCTAGCT1080.3791602303047325No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACCT1060.37213874455834856No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAC1060.37213874455834856No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACCTAGCT1060.37213874455834856No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG1060.37213874455834856No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG1060.37213874455834856No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTACCT1040.3651172588119646No Hit
AAGCGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTACC1010.3545850301923887No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCACGAGACCTAGAGGAGCCTGT980.3440528015728128No Hit
AAGCGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG930.32649908720685294No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACCT920.32298834433366097No Hit
AAGCGACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTCTA890.3124561157140851No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACCTAG860.30192388709450924No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT850.29841314422131726No Hit
AAGCGACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTACCTAGC840.2949024013481253No Hit
AAGCGACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTACCTAGC830.2913916584749333No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG830.2913916584749333No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG810.28437017272854936No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG780.27383794410897344No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCTAGCT780.27383794410897344No Hit
AAGCGACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTACCTA770.27032720123578147No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAC750.2633057154893976No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACCTAGCT720.25277348686982165No Hit
AAGCGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC670.2352197725038618No Hit
AAGCGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACCTAG610.21415531526471002No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTACCT580.20362308664513412No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG580.20362308664513412No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT560.19660160089875017No Hit
AAGCGACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA540.18958011515236625No Hit
AAGCGACTGGGATTAGATACCCCGTACCTAGAGGAGCCTGTTCTACCTAG540.18958011515236625No Hit
AAGCGACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTACCTA540.18958011515236625No Hit
AAGCGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTACC540.18958011515236625No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTACCT520.1825586294059823No Hit
AAGCGACTGGGATTAGATACCCCACCAGACCTAGAGGAGCCTGTTCTACC480.16851565791321443No Hit
AAGCGACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTTCT480.16851565791321443No Hit
AAGCGACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCTG480.16851565791321443No Hit
AAGCGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACCTAG470.16500491504002246No Hit
AAGCGACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCCT450.1579834292936385No Hit
AAGCGACTGGGATTAGATACCCCGACCCATCTAGAGGAGCCTGTTCTACC390.13691897205448672No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG380.13340822918129477No Hit
AAGCGACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTACCTA380.13340822918129477No Hit
AAGCGACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTACCTA370.1298974863081028No Hit
AAGCGACTGGGATTAGATACCCCGAGATCGAAAGAGCGCTAGAGGAGCCT350.12287600056171885No Hit
AAGCGACTGGGATTAGATACCCCCGTCTAGAGGAGCCTGTTCTACCTAGC300.10532228619575904No Hit
AAGCGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTACCTAG300.10532228619575904No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG300.10532228619575904No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGGA108.421766E-4295.05
ATGGGAT108.421766E-4295.06
GCGACGG108.421766E-4295.03
GCGCTGG108.421766E-4295.03
CTGGAAT108.421766E-4295.07
GACTGGG27850.0295.05
TGGAATT108.421766E-4295.08
AGCGCTG108.421766E-4295.02
CGATGGG108.421766E-4295.04
TGGGATA303.6379788E-12295.08
ACTGGGA27850.0295.06
GGAATTA108.421766E-4295.09
CGACTGG28050.0295.04
GCGACTG28100.0294.475073
CTGGGAT27900.0294.471347
AAGCGAC28400.0293.44191
AGCGACT28200.0293.430852
TGGGATT27700.0293.402538
GGGATTA27850.0292.88159
GGGATAG351.2732926E-11252.857139
Produced by FastQC (version 0.11.5)