FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-126-5-7-5-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-126-5-7-5-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27555
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG897232.56033387769915No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG20717.515877336236618No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTATA18016.536018871348212No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACTGT13054.735982580293958No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG9133.313373253493014No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC8313.0157866086009797No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT5642.0468154599891126No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGCAC5632.0431863545635998No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG4041.466158591907095No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCACTG3921.4226093268009437No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAGCA3851.3972055888223553No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT3641.3209943748865904No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTATA3181.1540555253130103No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAGCAC3061.110506260206859No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG2771.0052622028669933No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATAAG2470.8963890401016149No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATAAGCACT2160.783886771910724No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAT2090.7584830339321358No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG2050.7439666122300853No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG1890.6859009254218835No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG1700.6169479223371439No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG1700.6169479223371439No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTATA1540.5588822355289421No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAT1400.5080747595717656No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTATA1330.4826710215931773No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG1220.44275086191253854No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATAA1160.42097622935946294No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATAAGCA1160.42097622935946294No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTATAAGC1150.4173471239339503No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATAAGCA1140.41371801850843765No Hit
AAGCGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTATAA1110.4028307022318998No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG1080.391943385955362No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTATAAGC1060.38468517510433675No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATAAGCAC1050.38105606967882416No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG1010.36653964797677374No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAT980.3556523317002359No Hit
AAGCGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTGT740.26855380148793323No Hit
AAGCGACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTATAA740.26855380148793323No Hit
AAGCGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTATA730.26492469606242064No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG720.261295590636908No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTATAA690.25040827436037016No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATAAGCAC670.24315006350934493No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG620.22500453638178186No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTATAA610.22137543095626927No Hit
AAGCGACTGGGATTAGATACCCCCGCCACCTAGAGGAGCCTGTTCTATAA570.20685900925421882No Hit
AAGCGACTGGGATTAGATACCCCATAATCCTAGAGGAGCCTGTTCTATAA560.20322990382870623No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT530.19234258755216838No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTATAA520.18871348212665578No Hit
AAGCGACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTATA480.17419706042460534No Hit
AAGCGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG480.17419706042460534No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTATAAGCAC460.1669388495735801No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT450.16330974414806748No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT440.1596806387225549No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTATAAGC440.1596806387225549No Hit
AAGCGACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTATAAGCA430.1560515332970423No Hit
AAGCGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTATAAG430.1560515332970423No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATAAGCAC420.15242242787152968No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG390.14153511159499182No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT380.1379060061694792No Hit
AAGCGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTATAAGC380.1379060061694792No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATGA370.13427690074396662No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG360.130647795318454No Hit
AAGCGACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTCTA360.130647795318454No Hit
AAGCGACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA360.130647795318454No Hit
AAGCGACTGGGATTAGATACCCCAGTAGCTAGAGGAGCCTGTTCTATAAG360.130647795318454No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT350.1270186898929414No Hit
AAGCGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTATAAGCAC350.1270186898929414No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATAAGCAC350.1270186898929414No Hit
AAGCGACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA330.11976047904191617No Hit
AAGCGACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTATA320.11613137361640355No Hit
AAGCGACTGGGATTAGATACCCCTGCGTGCCGCGACTAGAGGAGCCTGTT310.11250226819089093No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC310.11250226819089093No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG290.10524405733986573No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGCCGTAAACGATGGATA290.10524405733986573No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG290.10524405733986573No Hit
AAGCGACTGGGATTAGATACCCCAACAACCTAGAGGAGCCTGTTCTATAA280.10161495191435312No Hit
AAGCGACTGGGATTAGATACCCCCGAGAAGACCTAGAGGAGCCTGTTCTA280.10161495191435312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGGG27200.0295.000035
CTGGGAT27100.0295.07
CTGGATT108.4203185E-4295.07
GGGATTG206.076698E-8295.09
GACTGGA157.1640625E-6295.05
TGGGATT27000.0295.08
TGGGATA108.4203185E-4295.08
ACTGGGA27150.0295.06
ACTGGAT108.4203185E-4295.06
CGACTGG27400.0295.04
GCGACTG27500.0294.463623
GGGATTA26950.0293.90549
AGCGACT27600.0293.396762
AAGCGAC27750.0292.342351
GGGATAG150.002834684196.666679
CCTATTA200.006702292147.5295
TGGATTA200.006702292147.58
AAACCTT2350.059.00000435-39
CATTGAG303.3208562E-759.00000450-54
AACCGCT1550.059.00000460-64
Produced by FastQC (version 0.11.5)