FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-125-5-7-5-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-125-5-7-5-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27408
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG943034.40601284296555No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAAC19287.034442498540572No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG19116.972416812609457No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCCTG11044.028021015761821No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC8613.1414185639229424No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG8343.042907180385289No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTTCGC6772.4700817279626386No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAAC4991.820636310566258No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAACTTCG4861.7732049036777584No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT4401.6053706946876825No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG3751.368213660245184No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT3141.1456509048453005No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCGCCT2901.058085230589609No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG2811.0252481027437244No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACTTCG2380.86835960303561No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG2310.8428196147110333No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAAC2190.7990367775831875No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAACTTCGCC2150.7844424985405721No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAACTT1780.6494454173963806No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTTCGC1710.6239054290718038No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG1710.6239054290718038No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG1640.5983654407472271No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAACTTCG1390.5071511967308815No Hit
AAGCGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA1320.4816112084063047No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG1320.4816112084063047No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAACTTCGC1240.45242265032107415No Hit
AAGCGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAACTTC1180.4305312317571512No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG1120.4086398131932283No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAACTTCG1050.3830998248686515No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAACT1030.37580268534734385No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAACTTC990.36120840630472856No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG990.36120840630472856No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAA930.3393169877408056No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG870.31742556917688264No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT840.3064798598949212No Hit
AAGCGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG840.3064798598949212No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAACT840.3064798598949212No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC820.29918272037361354No Hit
AAGCGACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAACTT820.29918272037361354No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG720.2626970227670753No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAACTTCGC710.2590484530064215No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAA680.24810274372446No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG670.2444541739638062No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAAC630.22985989492119088No Hit
AAGCGACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTAAC610.22256275539988324No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAACT550.2006713368359603No Hit
AAGCGACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAACTT530.19337419731465266No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAACTTC530.19337419731465266No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAA510.18607705779334502No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT470.1714827787507297No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGTT460.1678342089900759No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG460.1678342089900759No Hit
AAGCGACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTAACTTCG430.15688849970811442No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT430.15688849970811442No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAA410.14959136018680677No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT410.14959136018680677No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCCTG390.14229422066549913No Hit
AAGCGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAACT390.14229422066549913No Hit
AAGCGACTGGGATTAGATACCCCCCGAGACATAGAGGAGCCTGTTCTAAC390.14229422066549913No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG380.1386456509048453No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAACT370.13499708114419148No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCTA370.13499708114419148No Hit
AAGCGACTGGGATTAGATACCCCTGACCGACTAGAGGAGCCTGTTCTAAC370.13499708114419148No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAACTTCGC340.12405137186223No Hit
AAGCGACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAACTT340.12405137186223No Hit
AAGCGACTGGGATTAGATACCCCACTGTTGGAGACTAGAGGAGCCTGTTC340.12405137186223No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAACTTCGC330.12040280210157618No Hit
AAGCGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC330.12040280210157618No Hit
AAGCGACTGGGATTAGATACCCCAGCAGACCTAGAGGAGCCTGTTCTAAC310.11310566258026854No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAACTTCGC300.10945709281961472No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAACT300.10945709281961472No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG300.10945709281961472No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT290.1058085230589609No Hit
AAGCGACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA290.1058085230589609No Hit
AAGCGACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA280.10215995329830707No Hit
AAGCGACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTAACTT280.10215995329830707No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG280.10215995329830707No Hit
AAGCGACTGGGATTAGATACCCCTGACGCTTCCTAGAGGAGCCTGTTCTA280.10215995329830707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT27150.0295.07
CTGGATT157.163637E-6295.07
TGGATTA157.163637E-6295.08
GACTGGG27150.0295.05
ACTGGGA27150.0295.06
CGACTGG27300.0295.04
TGGGATA108.42007E-4294.999978
AAGCGAC27450.0294.462651
AGCGACT27400.0294.461672
GGGATTA27100.0294.455729
TGGGATT27100.0294.455728
GCGACTG27400.0293.923343
GACTGGA202.2579787E-5221.249985
ACTGGAT202.2579787E-5221.249986
GGGATAG150.0028346196.666679
ATACGAG200.006702095147.49998295
ATCTCTG150.00437228759.000004170-174
GCCTCAG2950.059.000004115-119
AGCTCGT150.00437228759.000004195-199
CAGTTAA11050.059.00000445-49
Produced by FastQC (version 0.11.5)