FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-124-5-1-3-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-124-5-1-3-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27297
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC847331.040041030149833No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA25439.31604205590358No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT17416.377990255339415No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCC13294.868666886471041No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT7982.923398175623695No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT7032.575374583287541No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTTC5662.0734879290764554No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT4941.8097226801480015No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA3891.4250650254606734No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAACTT3881.421401619225556No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC3561.3041726197017987No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAACTTCG3421.252884932410155No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTTC3081.128329120416163No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCGC2851.0440707770084625No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT2660.9744660585412316No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC2360.8645638714877092No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT2010.7363446532585999No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1870.6850569659669561No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT1870.6850569659669561No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT1790.6557497160860168No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAA1720.6301058724401949No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACTT1650.604462028794373No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1630.5971352163241381No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA1600.5861449976187859No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT1540.5641645602080815No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT1540.5641645602080815No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC1300.4762428105652636No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAA1200.43960874821408946No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAACT1120.41030149833315016No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1080.3956478733926805No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT1030.37733084221709345No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT970.355350404806389No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT970.355350404806389No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT930.34069677986591934No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTAAC890.32604315492544966No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAACTT870.31871634245521485No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAACTT830.30406271751474523No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT790.28940909257427555No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAA770.28208228010404074No Hit
ATCGGTTACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAAC760.27841887386892333No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAACTTC740.2710920613986885No Hit
ATCGGTTACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA710.26010184269333625No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT690.25277503022310144No Hit
ATCGGTTACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAG660.2417848115177492No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC660.2417848115177492No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA650.2381214052826318No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAA650.2381214052826318No Hit
ATCGGTTACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAACT630.23079459281239698No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAC590.2161409678719273No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG550.20148734293145767No Hit
ATCGGTTACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA540.19782393669634024No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT530.19416053046122284No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAACTTC520.19049712422610543No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAACTTC520.19049712422610543No Hit
ATCGGTTACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTA510.18683371799098802No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGCTAGAGGAGCCTGTTCTAACT500.18317031175587062No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG490.17950690552075318No Hit
ATCGGTTACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA470.17218009305051837No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAACT450.16485328058028356No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC430.15752646811004872No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAACTT420.15386306187493132No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAA420.15386306187493132No Hit
ATCGGTTACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAACTTC400.14653624940469648No Hit
ATCGGTTACTGGGATTAGATACCCCTATAAGAGGAGCCTGTTCTAACTTC390.14287284316957907No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT380.13920943693446167No Hit
ATCGGTTACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA370.13554603069934426No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAACT370.13554603069934426No Hit
ATCGGTTACTGGGATTAGATACCCCGGCCCACGAGACCTAGAGGAGCCTG330.1208924057588746No Hit
ATCGGTTACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTC310.11356559328863979No Hit
ATCGGTTACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAA300.10990218705352237No Hit
ATCGGTTACTGGGATTAGATACCCCGACCAACCTAGAGGAGCCTGTTCTA300.10990218705352237No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT300.10990218705352237No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC300.10990218705352237No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT280.10257537458328754No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTC108.4198156E-4295.02
TCGGTTA26900.0295.02
TCTGGGA108.4198156E-4295.07
GTTCTGG108.4198156E-4295.05
TTCTGGG108.4198156E-4295.06
GGTTCTG108.4198156E-4295.04
ATCGGTT27000.0295.01
CGGTACT255.16593E-10295.03
CGGTTAC26900.0294.451663
GGTTACT26800.0294.449624
GTTACTG26750.0294.448585
TTACTGG26750.0293.89726
TACTGGG26750.0291.69167
ACTGGGA26700.0291.68548
CTGGGAT26800.0290.597029
GTACTGG301.540684E-9245.833345
CTGGATT202.2578426E-5221.259
TACTGGA202.2578426E-5221.257
TCGGTAC353.8726284E-9210.714282
CGGTTCT150.0028345147196.666673
Produced by FastQC (version 0.11.5)