FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-123-6-4-8-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-123-6-4-8-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26781
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA1209745.170083267988495No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA20117.509054927000486No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCG14975.589783801949142No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA14595.447892162353908No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA11294.215675292184757No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGA5952.221724356820134No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA5372.0051529069116167No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT5231.9528770396923194No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA5001.866995257832045No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA4901.8296553526754042No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA4881.822187371644076No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCTGTAAACCTTGA4871.8184533811284118No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCGTAAACATTGA4681.7475075613307942No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCCAAA4381.6354878458608717No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCT3961.4786602442029797No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTTA3701.3815764907957133No Hit
TGATTGACACTGGGATTAGATACCCCACGAGAGGAGCCTGTTCTAGGAAT2560.955901572010007No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA2560.955901572010007No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATC1650.6161084350845749No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCC1430.5339606437399649No Hit
TGATTGACACTGGGATTAGATACCCCGGACAAGGACAAGGAGTTGCACCA1410.5264926627086367No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAA1030.3846010231134013No Hit
TGATTGACACTGGGATTAGATACCCCAAGCAGAAGACGGCATACGAGATC780.29125126022179904Illumina Single End Adapter 1 (96% over 25bp)
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC670.2501773645494941No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC510.1904335162988686No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC380.14189163959523543No Hit
TGATTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC340.12695567753257905No Hit
TGATTGACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC300.1120197154699227No Hit
TGATTGACACTGGGATTAGATACCCCATGTCAGACTAGAGGAGCCTGTTC280.10455173443859453No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGG108.418951E-4295.07
GATTGAC26400.0295.02
TTGACAC26350.0295.04
ATTGAAC108.418951E-4295.03
TTGAACT108.418951E-4295.04
TGAACTG108.418951E-4295.05
TGATTAC157.161734E-6294.999971
GATTACA157.161734E-6294.999972
TGATTGA26550.0294.444461
ATTGACA26450.0294.442353
GACACTG26300.0294.439156
ACACTGG26500.0292.773567
TGACACT26450.0292.769385
CACTGGG26350.0292.760938
ACTGGGA26450.0291.654089
CACTGGA150.0028342248196.666668
GAACTGG150.0028342248196.666666
ACTGGAT150.0028342248196.666669
TTACACT255.4963733E-5177.04
GATTGAA200.0067012087147.52
Produced by FastQC (version 0.11.5)