FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-122-6-7-5-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-122-6-7-5-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26534
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1374851.81276852340393No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG15335.777493027813371No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG12834.835305645586795No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC9123.4370995703625535No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG7882.9697746287781714No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG6072.2876309640461296No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG4281.6130247983719002No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG4001.5074998115625236No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG3571.3454435818195523No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG3431.292681088414864No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT3111.172081103489862No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT3031.1419311072586116No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTACGCCCTAAACGATGATCG2921.1004748624406422No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG2590.9761061279867341No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG2050.7725936534257933No Hit
AAGCGACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAATAGT1860.7009874123765735No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA1680.6331499208562599No Hit
AAGCGACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACAG1370.5163186854601644No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG1290.4861686892289139No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG1090.4107936986507877No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT1060.39948745006406877No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG1040.3919499510062562No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCACGCCCTAAACGATGGATG1030.3881812014773498No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG1000.3768749528906309No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG970.365568704303912No Hit
AAGCGACTGGGATTAGATACCCCCGCAGCAAGCGCATCGCGAACACCGTG860.3241124594859426No Hit
AAGCGACTGGGATTAGATACCCCACTATGATTAGCCCTAAACCTCAACAG840.31657496042812994No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT810.305268711841411No Hit
AAGCGACTGGGATTAGATACCCCGGCCGGCACAGTGAGCTTGCCTGTGGC780.29396246325469205No Hit
AAGCGACTGGGATTAGATACCCCTCACCGAAAACAGGTATGAAGTTGAAA740.27888746513906687No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGACT730.27511871561016055No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC680.256274967965629No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGATTGAG660.2487374689078164No Hit
AAGCGACTGGGATTAGATACCCCAAGACAAGAAATTGATTTAAAATATAT590.22235622220547224No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT520.1959749755031281No Hit
AAGCGACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATAA480.18089997738750282No Hit
AAGCGACTGGGATTAGATACCCCGGCTGAACGCGTGGCGGCCCTCGTGGG450.16959372880078388No Hit
AAGCGACTGGGATTAGATACCCCATGCCCTGCGCATCCAGTTGGCGACGT440.1658249792718776No Hit
AAGCGACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACTATAATAG360.13567498304062714No Hit
AAGCGACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTAGATTGAGA350.13190623351172082No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGCCGTAAACGATGGATA350.13190623351172082No Hit
AAGCGACTGGGATTAGATACCCCCAAGCTTGTCGTAATAGGTAGCTGCGT340.1281374839828145No Hit
AAGCGACTGGGATTAGATACCCCGGCCGGCACAGTGATCTTGCCTGTGGC320.12059998492500187No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG280.10552498680937664No Hit
AAGCGACTGGGATTAGATACCCCGTACAGGGCGCCGTCCTTGTAGATCCC280.10552498680937664No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG280.10552498680937664No Hit
AAGCGACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT280.10552498680937664No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCCACGAGACTCTCCGATATCTC280.10552498680937664No Hit
AAGCGACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT270.10175623728047034No Hit
AAGCGACTGGGATTAGATACCCCTGTACCCTCAAATTCTTCACCATCTTC270.10175623728047034No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGA108.418506E-4295.06
GGGTTAG108.418506E-4295.09
GCGACTG26200.0295.03
CTGGGTT108.418506E-4295.07
GCGCTGG108.418506E-4295.03
CTGTGAT108.418506E-4295.07
GACTGGG25950.0295.05
AAGCGCT108.418506E-4295.01
GGGATAG108.418506E-4295.09
TGGGTTA108.418506E-4295.08
AGCGCTG108.418506E-4295.02
TGGGATA108.418506E-4295.08
AGCGACT26350.0295.02
GCGACTT157.1609775E-6294.999973
ACTGGGA25900.0294.43056
CGACTGG26200.0293.874054
AAGCGAC26500.0293.33021
CTGGGAT25950.0293.29487
TGGGATT25850.0293.28828
GGGATTA25800.0293.284889
Produced by FastQC (version 0.11.5)