FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-121-5-5-7-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-121-5-5-7-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26515
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC632023.835564774655854No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA278210.492174240995663No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCT17066.434093909108052No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT12424.684141052234584No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACT9023.40184801056006No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA7352.7720158400905146No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA6192.334527625872148No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT5532.085611917782387No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCAC5502.0742975674146713No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT5091.9196681123892136No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCAC4621.7424099566283238No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCACT3341.259664340939091No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT3191.2030925891005093No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT3111.172920988119932No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC2821.0635489345653404No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC2731.0296058834621913No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCC2610.9843484819913256No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAC2280.8598906279464453No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT2260.8523477277013012No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT2160.8146332264755798No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT1880.7090326230435602No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACC1690.6373750707146898No Hit
CTGGATGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAC1680.6336036205921176No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACCACT1660.6260607203469734No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCACTC1650.6222892702244013No Hit
CTGGATGACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTACCA1460.5506317178955308No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCAC1330.5016028663020932No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1290.48651706581180465No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1270.47897416556666034No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAC1260.47520271544408826No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACCA1240.467659815198944No Hit
CTGGATGACTGGGATTAGATACCCCCCGAGACCTAGAGGAGCCTGTTCTA1100.4148595134829342No Hit
CTGGATGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTACCACT1050.3960022628700735No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTACC990.3733735621346408No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT960.3620592117669244No Hit
CTGGATGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTA960.3620592117669244No Hit
CTGGATGACTGGGATTAGATACCCCTGTAACTAGAGGAGCCTGTTCTACC930.350744861399208No Hit
CTGGATGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA920.3469734112766359No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC890.3356590609089195No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT830.3130303601734867No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT810.3054874599283425No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCACT790.2979445596831982No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT780.2941731095606261No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAC760.2866302093154818No Hit
CTGGATGACTGGGATTAGATACCCCGACGACTAGAGGAGCCTGTTCTACC710.2677729587026212No Hit
CTGGATGACTGGGATTAGATACCCCACGAACCTAGAGGAGCCTGTTCTAC660.24891570808976052No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAC660.24891570808976052No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCAC660.24891570808976052No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAC640.24137280784461626No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT610.23005845747689985No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT590.22251555723175562No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTACCA580.21874410710918346No Hit
CTGGATGACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGT580.21874410710918346No Hit
CTGGATGACTGGGATTAGATACCCCTGACCTAGAGGAGCCTGTTCTACCA570.21497265698661133No Hit
CTGGATGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT560.21120120686403923No Hit
CTGGATGACTGGGATTAGATACCCCGAGATCTAGAGGAGCCTGTTCTACC560.21120120686403923No Hit
CTGGATGACTGGGATTAGATACCCCCACGACCTAGAGGAGCCTGTTCTAC540.20365830661889495No Hit
CTGGATGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCACT510.19234395625117856No Hit
CTGGATGACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTA500.18857250612860646No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT500.18857250612860646No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG500.18857250612860646No Hit
CTGGATGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACCACT480.1810296058834622No Hit
CTGGATGACTGGGATTAGATACCCCGCACGGACCTAGAGGAGCCTGTTCT480.1810296058834622No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT470.17725815576089005No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC470.17725815576089005No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG470.17725815576089005No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT450.16971525551574582No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCACT440.16594380539317366No Hit
CTGGATGACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTACC410.1546294550254573No Hit
CTGGATGACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTACCA400.15085800490288515No Hit
CTGGATGACTGGGATTAGATACCCCACAACCTAGAGGAGCCTGTTCTACC400.15085800490288515No Hit
CTGGATGACTGGGATTAGATACCCCGAAACCTAGAGGAGCCTGTTCTACC380.1433151046577409No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCACTAGAGGAGCCTGTTCTAC380.1433151046577409No Hit
CTGGATGACTGGGATTAGATACCCCCACGGACCTAGAGGAGCCTGTTCTA370.1395436545351688No Hit
CTGGATGACTGGGATTAGATACCCCGCCACGAGACCTAGAGGAGCCTGTT360.13577220441259663No Hit
CTGGATGACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAC330.12445785404488026No Hit
CTGGATGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAC330.12445785404488026No Hit
CTGGATGACTGGGATTAGATACCCCCCGACCTAGAGGAGCCTGTTCTACC310.116914953799736No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA290.10937205355459173No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA270.10182915330944747No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATT108.418506E-4295.09
CTGGAGA108.418506E-4295.01
ACGGGAT108.418506E-4295.08
TGACGGG108.418506E-4295.06
GGATGAC26400.0294.441283
TGACTGG26250.0294.438086
GACTGGG26250.0293.87627
ACTGGGA26250.0293.87628
CTGGGAT26150.0293.87199
CTGGATG26550.0293.333341
TGGATGA26550.0292.777772
GATGACT26500.0292.216984
ATGACTG26450.0292.21175
GACGGGA150.0028340758196.666667
ATGACGG200.0067008566147.55
TGCTCGC6250.059.00000465-69
GCTCGCC7200.059.00000465-69
ACCCCTC452.7284841E-1159.00000420-24
CTGCTCG6250.059.00000465-69
ATACCCC26300.059.00000415-19
Produced by FastQC (version 0.11.5)