FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-120-5-8-2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-120-5-8-2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26945
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA718926.680274633512713No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG22978.524772685099276No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA16045.952866951196882No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAAC13254.9174243830024125No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTGAG8433.12859528669512No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG6722.493969196511412No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA6462.3974763406940065No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA6392.3714974948970124No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG5171.9187233252922622No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAG4151.5401744293932085No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGAA4081.5141955835962144No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTGAG3961.4696604193727965No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT3211.1913156429764336No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA3021.1208016329560215No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTGA2931.087400259788458No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA2911.0799777324178883No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC2650.9834848766004824No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA2470.9166821302653554No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGAGA2220.8239005381332343No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGCT1900.7051401002041195No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA1810.671738727036556No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAG1590.5900909259602894No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1580.5863796622750046No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCTGA1350.5010205975134533No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA1330.49359807014288365No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA1160.4305065874930414No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA1040.3859714232696233No Hit
CAACACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGC1020.3785488958990536No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC1000.37112636852848396No Hit
CAACACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGCTG970.3599925774726294No Hit
CAACACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGCT950.3525700501020598No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT910.3377249953609204No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA910.3377249953609204No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCTGA900.33401373167563553No Hit
CAACACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGCTGAG860.3191686769344962No Hit
CAACACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT850.31545741324921134No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC800.29690109482278715No Hit
CAACACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGC760.2820560400816478No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC760.2820560400816478No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA750.27834477639636296No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA740.2746335127110781No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA710.2634997216552236No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGT680.2523659305993691No Hit
CAACACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGC670.24865466691408428No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA640.23752087585822973No Hit
CAACACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGCTGAG630.2338096121729449No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA620.23009834848766006No Hit
CAACACACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT620.23009834848766006No Hit
CAACACACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTAGCTG610.2263870848023752No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA580.2152532937465207No Hit
CAACACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAG530.1966969753200965No Hit
CAACACACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCCT530.1966969753200965No Hit
CAACACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGCTG530.1966969753200965No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAGC530.1966969753200965No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA520.19298571163481165No Hit
CAACACACTGGGATTAGATACCCCATAATCCTAGAGGAGCCTGTTCTAGC500.18556318426424198No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT490.18185192057895713No Hit
CAACACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAG460.1707181295231026No Hit
CAACACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGCTGAG460.1707181295231026No Hit
CAACACACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCC450.16700686583781776No Hit
CAACACACTGGGATTAGATACCCCTCCGACCCACGAGACCTAGAGGAGCC430.1595843384672481No Hit
CAACACACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAGCT420.15587307478196324No Hit
CAACACACTGGGATTAGATACCCCAATGTCTAGAGGAGCCTGTTCTAGCT420.15587307478196324No Hit
CAACACACTGGGATTAGATACCCCAACGAACCTAGAGGAGCCTGTTCTAG410.15216181109667842No Hit
CAACACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGCTGAG410.15216181109667842No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA400.14845054741139357No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG390.14473928372610873No Hit
CAACACACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCTAG380.1410280200408239No Hit
CAACACACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAG370.13731675635553905No Hit
CAACACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGCTGA360.1336054926702542No Hit
CAACACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGCTGA360.1336054926702542No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGC340.12618296529968456No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGC340.12618296529968456No Hit
CAACACACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCT330.12247170161439971No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG330.12247170161439971No Hit
CAACACACTGGGATTAGATACCCCCCTAACACTAGAGGAGCCTGTTCTAG330.12247170161439971No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA330.12247170161439971No Hit
CAACACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGCTGAG320.11876043792911486No Hit
CAACACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGCTG320.11876043792911486No Hit
CAACACACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAG300.1113379105585452No Hit
CAACACACTGGGATTAGATACCCCATGCTAGAGGAGCCTGTTCTAGCTGA280.10391538318797551No Hit
CAACACACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAGCT270.10020411950269068No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT108.4192137E-4295.07
ACACGGG108.4192137E-4295.05
CACACGG108.4192137E-4295.04
ACATGGG108.4192137E-4295.05
CTGGATT108.4192137E-4295.08
CATGGGA108.4192137E-4295.06
CGCTGGG157.1621816E-6294.999976
ACGCTGG157.1621816E-6294.999975
TGGGATA157.1621816E-6294.999979
CACTGGG26250.0294.438086
CTGGGAT26450.0293.884678
ACTGGGA26250.0293.87627
ACACTGG26500.0292.77365
TGGGATT26400.0292.765149
CACACTG26900.0288.420074
ACACACT28000.0277.61613
AACACAC33050.0236.089252
CAACACA33300.0235.645661
CACGCTG202.2575203E-5221.254
GCTGGGA202.2575203E-5221.257
Produced by FastQC (version 0.11.5)