FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-12-5-2-6-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-12-5-2-6-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11585
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA318727.50971083297367No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC9338.053517479499353No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA7776.706948640483383No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCTAGCTGT6105.265429434613725No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA3733.21968062149331No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA3322.8657747086750107No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCTAGC2692.321968062149331No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAC2322.0025895554596462No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCTAG2301.985325852395339No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT1781.536469572723349No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCTAGCT1651.4242555028053518No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1651.4242555028053518No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1511.3034095813552007No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCTAGCTG1441.2429866206301252No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAC1251.078981441519206No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1251.078981441519206No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACCT1211.0444540353905913No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA1201.0358221838584376No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1100.9495036685369012No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCTAG980.8459214501510574No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA950.8200258955545966No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA820.7078118256365991No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCTAGC740.6387570133793699No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACCTAGC720.6214933103150626No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC660.5697022011221407No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAC630.5438066465256798No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA620.5351747949935262No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA590.5092792403970652No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA540.46611998273629696No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA540.46611998273629696No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA530.4574881312041433No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA500.43159257660768235No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCTAG490.4229607250755287No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTACC460.39706517047906775No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAC450.38843331894691413No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTACC450.38843331894691413No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACC430.37116961588260683No Hit
GCCACAACTGGGATTAGATACCCCGAGATGCCTAAGCTAGAGGAGCCTGT420.36253776435045315No Hit
GCCACAACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACC400.3452740612861459No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC390.3366422097539922No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA380.3280103582218386No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA350.3021148036253776No Hit
GCCACAACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTAC340.293482952093224No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT320.2762192490289167No Hit
GCCACAACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA290.2503236944324558No Hit
GCCACAACTGGGATTAGATACCCCTACCGTAGATACATGACTTTACCTAG260.22442813983599483No Hit
GCCACAACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACCTA240.20716443677168753No Hit
GCCACAACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA230.19853258523953388No Hit
GCCACAACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAC230.19853258523953388No Hit
GCCACAACTGGGATTAGATACCCCGACCAACCTAGAGGAGCCTGTTCTAC220.18990073370738023No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC220.18990073370738023No Hit
GCCACAACTGGGATTAGATACCCCGACCCGACCTAGAGGAGCCTGTTCTA220.18990073370738023No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACCTAGCT210.18126888217522658No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGT200.17263703064307295No Hit
GCCACAACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGAG200.17263703064307295No Hit
GCCACAACTGGGATTAGATACCCCCGACCAACCTAGAGGAGCCTGTTCTA200.17263703064307295No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTACC190.1640051791109193No Hit
GCCACAACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTACC190.1640051791109193No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA190.1640051791109193No Hit
GCCACAACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCT170.146741476046612No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT170.146741476046612No Hit
GCCACAACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTTC170.146741476046612No Hit
GCCACAACTGGGATTAGATACCCCCGAGCCCTAGAGGAGCCTGTTCTACC170.146741476046612No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA170.146741476046612No Hit
GCCACAACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTACCT160.13810962451445835No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTACC160.13810962451445835No Hit
GCCACAACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTACC160.13810962451445835No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCT150.1294777729823047No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA150.1294777729823047No Hit
GCCACAACTGGGATTAGATACCCCGGCCCACACCTAGAGGAGCCTGTTCT150.1294777729823047No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG150.1294777729823047No Hit
GCCACAACTGGGATTAGATACCCCTAGATCGGAAGAGCGCTAGAGGAGCC140.12084592145015105No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA140.12084592145015105No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATG140.12084592145015105No Hit
GCCACAACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTACCTA140.12084592145015105No Hit
GCCACAACTGGGATTAGATACCCCATGTCAGACTAGAGGAGCCTGTTCTA140.12084592145015105No Hit
GCCACAACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGG130.11221406991799741No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT120.10358221838584376No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGG11350.0295.06
CTGGGAT11350.0295.08
CAACTGG11400.0295.05
ACTGGGA11350.0295.07
TGGGATT11350.0293.700449
GCCACAA11550.0292.44591
CCACAAC12100.0279.15292
ACAACTG12100.0279.15294
CACAACT12150.0278.004123
CCTGTAG452.5465852E-1159.00000420-24
CCGCCAG1650.059.00000465-69
CTTAGCC3550.059.00000430-34
GTCCCTA452.5465852E-1159.00000425-29
CCCCGTC452.5465852E-1159.00000420-24
TATCCGC1650.059.00000465-69
ATGCTTA3600.059.00000425-29
ACCCACC1750.059.000004125-129
CCCCTGT452.5465852E-1159.00000420-24
TACCCCG1750.059.00000415-19
CTATGCT3700.059.00000425-29
Produced by FastQC (version 0.11.5)